All Phenotypes Lgr5<+> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Lgr5⁺
wild type
MGI:2437965

Summary

31 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Lgr5^{em1(IMPC)Kmpc}/Lgr5⁺	C57BL/6NTac-Lgr5^{em1(IMPC)Kmpc}/Kmpc	MGI:7472084
ht2	Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C57BL/6	MGI:5559881
ht3	Lgr5^{tm2(Hbegf/EGFP)Fjs}/Lgr5⁺	involves: C57BL/6N	MGI:5298068
cn4	Rnf43^tm1Cle/Rnf43^tm1Cle Znrf3^tm1Cle/Znrf3^tm1Cle Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd	MGI:5439368
cn5	Slc39a7^tm1.1Tfk/Slc39a7^tm1.1Tfk Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5902448
cn6	Rab11a^tm1.1Ngao/Rab11a^tm1.1Ngao Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S4/SvJae	MGI:5608789
cn7	Tigar^{Gt(EUCE0047g05)Hmgu}/Tigar^{Gt(EUCE0047g05)Hmgu} Apc^tm1Tno/Apc^tm1Tno Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S4/SvJae	MGI:5522716
cn8	Apc^tm1Tno/Apc^tm1Tno Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6	MGI:5898006
cn9	Apc^tm1Tno/Apc^tm1Tno Elp3^tm1.1Tac/Elp3^tm1.1Tac Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * C57BL/6NTac	MGI:5898005
cn10	Col1a1^{tm2(tetO-Pou5f1)Jae}/Col1a1⁺ Gt(ROSA)26Sor^tm1(tTA)Roos/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * DBA/2J	MGI:5508640
cn11	Col1a1^{tm1(tetO-SOX2)Mjm}/Col1a1⁺ Gt(ROSA)26Sor^tm1(tTA)Roos/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * DBA/2J	MGI:5508638
cn12	Gata6os^em3Zfa/Gata6os^em3Zfa Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6	MGI:6367573
cn13	Gata6os^em1Zfa/Gata6os^em1Zfa Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6	MGI:6367570
cn14	Nrbp1^tm1.1Dja/Nrbp1^tm1.2Dja Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J	MGI:5427571
cn15	Ctnnb1^tm1Mmt/Ctnnb1⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129X1/SvJ	MGI:5475209
cn16	Cdc42^tm1Brak/Cdc42^tm1Brak Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6	MGI:5427870
cn17	Hmgcs2^tm1.1Yil/Hmgcs2^tm1.1Yil Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C3H * C57BL/6 * C57BL/6J	MGI:8159407
cn18	Cdx2^tm2Fbe/Cdx2^tm2Fbe Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C57BL/6	MGI:5308972
cn19	Bptf^em1Zfa/Bptf^em1Zfa Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C57BL/6	MGI:6367576
cn20	Bptf^em1Zfa/Bptf^em1Zfa Ehf^em1Zfa/Ehf^em1Zfa Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C57BL/6	MGI:6367579
cn21	Gata6os^em1Zfa/Gata6os^em1Zfa Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C57BL/6	MGI:6367565
cn22	Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ Stk26^tm2.1Zzh/Stk26^tm2.1Zzh	involves: 129P2/OlaHsd * C57BL/6	MGI:7491700
cn23	Apc^tm2.1Cip/Apc⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C57BL/6	MGI:5432137
cn24	Cdc42^tm1Brak/Cdc42^tm1Brak Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C57BL/6	MGI:5427869
cn25	Elp3^tm1.1Tac/Elp3^tm1.1Tac Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NTac	MGI:5898004
cn26	Rprd1b^tm1Tshu/Rprd1b^tm1Tshu Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ Tg(Vil1-cre)20Syr/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2	MGI:6822318
cn27	Rpap3^{tm1c(KOMP)Wtsi}/Rpap3^{tm1c(KOMP)Wtsi} Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺	involves: 129P2/OlaHsd * C57BL/6N	MGI:7310428
cn28	Hmgcs2^tm1.1Yil/Hmgcs2^tm1.1Yil Lgr5^{tm3(cre/ERT2)Cle}/Lgr5⁺ Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺	involves: 129S6/SvEvTac * C3H * C57BL/6J * C57BL/6NCrl	MGI:8159410
cn29	Lgr5^{tm3(cre/ERT2)Cle}/Lgr5⁺ Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺	involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl	MGI:8159416
cx30	Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1^{tm1.1(cre/ERT2)Rjc}	involves: 129 * 129P2/OlaHsd * C57BL/6	MGI:5432135
cx31	Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ Mex3a^{tm1.1(KOMP)Vlcg}/Mex3a⁺	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * C57BL/6NTac	MGI:6430341

Allelic Composition	Lgr5^{em1(IMPC)Kmpc}/Lgr5⁺
Genetic Background	C57BL/6NTac-Lgr5^{em1(IMPC)Kmpc}/Kmpc

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{em1(IMPC)Kmpc} mutation (0 available); any Lgr5 mutation (58 available)

Data Sources

IMPC Data for Lgr5

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

increased grip strength ( J:211773 )

IMPC - KMPC

no spontaneous movement ( J:211773 )

IMPC - KMPC

hematopoietic system

increased CD11b-high dendritic cell number ( J:211773 )

IMPC - KMPC

increased CD8-positive, CD25-positive alpha-beta T cell number ( J:211773 )

IMPC - KMPC

immune system

increased CD11b-high dendritic cell number ( J:211773 )

IMPC - KMPC

increased CD8-positive, CD25-positive alpha-beta T cell number ( J:211773 )

IMPC - KMPC

Allelic Composition	Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

digestive/alimentary phenotype ( J:285672 )

N	• mice exhibit normal intestinal morphology and physiology under basal conditions and normal response to irradiation

abnormal intestinal epithelium physiology ( J:285672 )

• intestinal stem cells treated with tamoxifen exhibit impaired organoid formation compared with control cells

Allelic Composition	Lgr5^{tm2(Hbegf/EGFP)Fjs}/Lgr5⁺
Genetic Background	involves: C57BL/6N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm2(Hbegf/EGFP)Fjs} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

digestive/alimentary phenotype ( J:177145 )

N	• diphtheria-treated mice exhibit normal intestinal epithelial migration

Allelic Composition	Rnf43^tm1Cle/Rnf43^tm1Cle Znrf3^tm1Cle/Znrf3^tm1Cle Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Rnf43^tm1Cle mutation (0 available); any Rnf43 mutation (36 available)
Znrf3^tm1Cle mutation (0 available); any Znrf3 mutation (37 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

neoplasm

increased intestinal adenoma incidence ( J:186765 )

• form after tamoxifen treatment and contain Olfm4+ stem cells and Paneth cells

digestive/alimentary system

increased intestinal adenoma incidence ( J:186765 )

• form after tamoxifen treatment and contain Olfm4+ stem cells and Paneth cells

Allelic Composition	Slc39a7^tm1.1Tfk/Slc39a7^tm1.1Tfk Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Slc39a7^tm1.1Tfk mutation (0 available); any Slc39a7 mutation (28 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

increased mortality induced by gamma-irradiation ( J:236234 )

• all tamoxifen-treated mice die within 4 days of gamma irradiation

digestive/alimentary system

digestive/alimentary phenotype ( J:236234 )

N	• tamoxifen-treated mice exhibit normal epithelial organoid and crypt-derived epithelial organoid formation

abnormal intestine development ( J:236234 )

• tamoxifen-treated mice exhibit massive apoptosis of transit-amplifying cells due to increased ER stress

decreased Paneth cell number ( J:236234 )

• degeneration of Paneth cells in tamoxifen-treated mice

homeostasis/metabolism

increased mortality induced by gamma-irradiation ( J:236234 )

• all tamoxifen-treated mice die within 4 days of gamma irradiation

endocrine/exocrine glands

decreased Paneth cell number ( J:236234 )

• degeneration of Paneth cells in tamoxifen-treated mice

Allelic Composition	Rab11a^tm1.1Ngao/Rab11a^tm1.1Ngao Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Rab11a^tm1.1Ngao mutation (1 available); any Rab11a mutation (14 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal intestinal epithelium morphology ( J:215917 )

• increase in proliferation of intestinal epithelial stem cells and the transit-amplifying cells following tamoxifen induction

Allelic Composition	Tigar^{Gt(EUCE0047g05)Hmgu}/Tigar^{Gt(EUCE0047g05)Hmgu} Apc^tm1Tno/Apc^tm1Tno Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apc^tm1Tno mutation (5 available); any Apc mutation (156 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Tigar^{Gt(EUCE0047g05)Hmgu} mutation (0 available); any Tigar mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:198650 )

• in tamoxifen-treated mice compared with Apc^tm1Tno/Apc^tm1Tno Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ mice

neoplasm

decreased tumor growth/size ( J:198650 )

• tamoxifen-treated mice exhibit a reduced total tumor burden and average tumor size of abnormally proliferating adenomas in the small intestine and reduced size, but not number, of colon adenomas due to reduced proliferation and increased reactive oxygen species damage compared with Apc^tm1Tno/Apc^tm1Tno Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ mice

Allelic Composition	Apc^tm1Tno/Apc^tm1Tno Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

increased intestinal adenoma incidence ( J:227334 )

• tamoxifen treated mice develop numerous hyperplasias in the intestine

neoplasm

increased intestinal adenoma incidence ( J:227334 )

• tamoxifen treated mice develop numerous hyperplasias in the intestine

Allelic Composition	Apc^tm1Tno/Apc^tm1Tno Elp3^tm1.1Tac/Elp3^tm1.1Tac Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * C57BL/6NTac

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apc^tm1Tno mutation (5 available); any Apc mutation (156 available)
Elp3^tm1.1Tac mutation (0 available); any Elp3 mutation (40 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal intestinal epithelium morphology ( J:227334 )

• the amount of Dclk1+/Sox9+ cells in the Lgr5+ population is decreased in ex vivo organoids

abnormal small intestinal crypt cell physiology ( J:227334 )

• intestinal crypts fail to efficiently generate spheroid structures ex vivo

neoplasm

decreased tumor growth/size ( J:227334 )

• tamoxifen treated mice exhibit decreased numbers of hyperplastic foci/polyps in the intestine indicating impaired tumor initiation

Allelic Composition	Col1a1^{tm2(tetO-Pou5f1)Jae}/Col1a1⁺ Gt(ROSA)26Sor^tm1(tTA)Roos/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * DBA/2J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col1a1^{tm2(tetO-Pou5f1)Jae} mutation (1 available); any Col1a1 mutation (166 available)
Gt(ROSA)26Sor^tm1(tTA)Roos mutation (3 available); any Gt(ROSA)26Sor mutation (1095 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal small intestine crypts of Lieberkuhn morphology ( J:199754 )

• expanded crypts in the ileum of doxycycline-treated mice

abnormal intestine physiology ( J:199754 )

• increased cell proliferation in the intestine of doxycycline-treated mice without an increase in Lgr5+ cells

cellular

increased cell proliferation ( J:199754 )

• in the intestine of doxycycline-treated mice without an increase in Lgr5+ cells

endocrine/exocrine glands

abnormal small intestine crypts of Lieberkuhn morphology ( J:199754 )

• expanded crypts in the ileum of doxycycline-treated mice

Allelic Composition	Col1a1^{tm1(tetO-SOX2)Mjm}/Col1a1⁺ Gt(ROSA)26Sor^tm1(tTA)Roos/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * DBA/2J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col1a1^{tm1(tetO-SOX2)Mjm} mutation (0 available); any Col1a1 mutation (166 available)
Gt(ROSA)26Sor^tm1(tTA)Roos mutation (3 available); any Gt(ROSA)26Sor mutation (1095 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

ectopic Paneth cells ( J:199754 )

• lyzsozyme+ Paneth cells in doxycycline-treated mice are not restricted to the base of the crypt unlike in control cells

abnormal ileum crypts of Lieberkuhn morphology ( J:199754 )

• expanded crypts in the ileum of doxycycline-treated mice

abnormal intestine physiology ( J:199754 )

• increased cell proliferation in the intestine of doxycycline-treated mice with an increase in intestinal stem Lgr5+ cells

• increased proliferation of intestinal stem cells in the intestine of doxycycline-treated mice

• expansion of intestinal stem cells is cell autonomous in doxycycline treated mice

cellular

increased cell proliferation ( J:199754 )

• in the intestine of doxycycline-treated mice with an increase in Lgr5+ intestinal stem cells

• increased proliferation of intestinal stem cells in the intestine of doxycycline-treated mice

• expansion of intestinal stem cells is cell autonomous in doxycycline treated mice

endocrine/exocrine glands

ectopic Paneth cells ( J:199754 )

• lyzsozyme+ Paneth cells in doxycycline-treated mice are not restricted to the base of the crypt unlike in control cells

abnormal ileum crypts of Lieberkuhn morphology ( J:199754 )

• expanded crypts in the ileum of doxycycline-treated mice

Allelic Composition	Gata6os^em3Zfa/Gata6os^em3Zfa Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata6os^em3Zfa mutation (0 available); any Gata6os mutation (0 available)
Gt(ROSA)26Sor^tm1Sor mutation (10 available); any Gt(ROSA)26Sor mutation (1095 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal enterocyte proliferation ( J:268724 )

• reduced cycling and proliferation of intestinal stem cells

• however, proliferation rates are normal

digestive/alimentary system

abnormal enterocyte proliferation ( J:268724 )

• reduced cycling and proliferation of intestinal stem cells

• however, proliferation rates are normal

Allelic Composition	Gata6os^em1Zfa/Gata6os^em1Zfa Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata6os^em1Zfa mutation (0 available); any Gata6os mutation (0 available)
Gt(ROSA)26Sor^tm1Sor mutation (10 available); any Gt(ROSA)26Sor mutation (1095 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal enterocyte physiology ( J:268724 )

• impaired intestinal epithelial renewal in tamoxifen treated mice

Allelic Composition	Nrbp1^tm1.1Dja/Nrbp1^tm1.2Dja Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Nrbp1^tm1.1Dja mutation (0 available); any Nrbp1 mutation (46 available)
Nrbp1^tm1.2Dja mutation (0 available); any Nrbp1 mutation (46 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal intestine morphology ( J:184685 )

• authors state that tamoxifen-treated mice exhibit similar phenotype as Nrbp1^tm1.1Dja/Nrbp1^tm1.1Dja Gt(ROSA)26Sor^{tm1(cre/ERT)Brn}/ Gt(ROSA)26Sor⁺ mice

abnormal intestinal goblet cell morphology ( J:184685 )

• abnormal goblet cell production in tamoxifen-treated mice

abnormal Paneth cell morphology ( J:184685 )

• abnormal granularization of Paneth cells in tamoxifen-treated mice

ectopic Paneth cells ( J:184685 )

• abnormal localization of Paneth cells in tamoxifen-treated mice

endocrine/exocrine glands

abnormal Paneth cell morphology ( J:184685 )

• abnormal granularization of Paneth cells in tamoxifen-treated mice

ectopic Paneth cells ( J:184685 )

• abnormal localization of Paneth cells in tamoxifen-treated mice

cellular

abnormal intestinal goblet cell morphology ( J:184685 )

• abnormal goblet cell production in tamoxifen-treated mice

Allelic Composition	Ctnnb1^tm1Mmt/Ctnnb1⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctnnb1^tm1Mmt mutation (0 available); any Ctnnb1 mutation (47 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

intestine polyps ( J:193873 )

• 40 days after tamoxifen treatment, mice have developed numerous polyps

Allelic Composition	Cdc42^tm1Brak/Cdc42^tm1Brak Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc42^tm1Brak mutation (0 available); any Cdc42 mutation (44 available)
Gt(ROSA)26Sor^tm1(EYFP)Cos mutation (23 available); any Gt(ROSA)26Sor mutation (1095 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

increased small intestinal crypt cell apoptosis ( J:184563 )

• intestinal crypt stem cells show increased cell death following tamoxifen administration

abnormal intestinal epithelium morphology ( J:184563 )

• 3 weeks after tamoxifen administration, mutant villus epithelial cells show disrupted cell polarity, as indicated by disorganized nuclear alignment

• stem cells contribute less to the villus epithelial compartments than in controls following tamoxifen administration

abnormal crypts of Lieberkuhn morphology ( J:184563 )

• 3 weeks after tamoxifen administration, more mutant stem cells in intestinal crypts undergo mitosis compared to control stem cells

• stem cells contribute less to the villus epithelial compartments than in controls following tamoxifen administration, indicating reduced clonal expansion of mutant stem cells

absent Paneth cells ( J:184563 )

• 2 weeks after tamoxifen administration, mutant stem cells in intestinal crypts fail to give rise to Paneth cells

abnormal small intestinal villus morphology ( J:184563 )

• 3 weeks after tamoxifen administration, mutant villus epithelial cells show disrupted cell polarity, as indicated by disorganized nuclear alignment

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:184563 )

• 3 weeks after tamoxifen administration, more mutant stem cells in intestinal crypts undergo mitosis compared to control stem cells

• stem cells contribute less to the villus epithelial compartments than in controls following tamoxifen administration, indicating reduced clonal expansion of mutant stem cells

absent Paneth cells ( J:184563 )

• 2 weeks after tamoxifen administration, mutant stem cells in intestinal crypts fail to give rise to Paneth cells

cellular

increased small intestinal crypt cell apoptosis ( J:184563 )

• intestinal crypt stem cells show increased cell death following tamoxifen administration

Allelic Composition	Hmgcs2^tm1.1Yil/Hmgcs2^tm1.1Yil Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C3H * C57BL/6 * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hmgcs2^tm1.1Yil mutation (1 available); any Hmgcs2 mutation (33 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal intestinal crypt stem cell physiology ( J:280699 )

• when co-cultured with wild-type Paneth cells, flow-sorted GFP^hi intestinal stem cells (ISCs) from tamoxifen (TAM)-treated mice form 42.9% fewer and 34.6% smaller organoids than wild-type ISCs, indicating reduced organoid-initiating capacity

decreased intestinal crypt stem cell number ( J:280699 )

• when adult mice are treated with TAM for 20 days, the % of Lgr5-GFP^hi ISCs in small intestinal crypts is reduced by 50% at 1 day after the last TAM injection

abnormal small intestine crypts of Lieberkuhn morphology ( J:280699 )

• when adult mice are treated with TAM for 20 days, the frequencies of Lgr5-GFP^hi ISCs and Lgr5-GFP^low progenitors in small intestinal crypts are reduced by 50% at 1 day after the last TAM injection

endocrine/exocrine glands

decreased intestinal crypt stem cell number ( J:280699 )

• when adult mice are treated with TAM for 20 days, the % of Lgr5-GFP^hi ISCs in small intestinal crypts is reduced by 50% at 1 day after the last TAM injection

abnormal small intestine crypts of Lieberkuhn morphology ( J:280699 )

cellular

abnormal intestinal crypt stem cell physiology ( J:280699 )

Allelic Composition	Cdx2^tm2Fbe/Cdx2^tm2Fbe Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdx2^tm2Fbe mutation (0 available); any Cdx2 mutation (21 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal crypts of Lieberkuhn morphology ( J:179732 )

• by 6 weeks after treatment cystic vesicles persist and are often filled with mucous and detritus

increased Paneth cell number ( J:179732 )

• an excess of Paneth cells are found by 19-62 weeks after treatment and these cells contain an abnormally abundant number of normal looking secretory granules

abnormal intestine physiology ( J:179732 )

• 4 weeks after treatment with tamoxifen a low level of excess mucous secretion is seen

abnormal Paneth cell physiology ( J:179732 )

• by 6 weeks after treatment dying Paneth cells are commonly seen

cellular

abnormal cell migration ( J:179732 )

• recombined cells fail to migrate out of the crypts and up the villi in the intestine

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:179732 )

• by 6 weeks after treatment cystic vesicles persist and are often filled with mucous and detritus

increased Paneth cell number ( J:179732 )

• an excess of Paneth cells are found by 19-62 weeks after treatment and these cells contain an abnormally abundant number of normal looking secretory granules

Allelic Composition	Bptf^em1Zfa/Bptf^em1Zfa Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bptf^em1Zfa mutation (0 available); any Bptf mutation (157 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal digestive system development ( J:268724 )

• reduced intestinal stem cells with impaired organoid formation capacity

Allelic Composition	Bptf^em1Zfa/Bptf^em1Zfa Ehf^em1Zfa/Ehf^em1Zfa Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bptf^em1Zfa mutation (0 available); any Bptf mutation (157 available)
Ehf^em1Zfa mutation (0 available); any Ehf mutation (26 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal digestive system development ( J:268724 )

• almost blocked organoid formation from intestinal stem cells

homeostasis/metabolism

abnormal response to radiation ( J:268724 )

• severely impaired intestinal epithelium regeneration after radiation injury compared with wild-type mice

Allelic Composition	Gata6os^em1Zfa/Gata6os^em1Zfa Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata6os^em1Zfa mutation (0 available); any Gata6os mutation (0 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal digestive system development ( J:268724 )

• reduced intestinal stem cells with impaired organoid formation capacity

• however, overexpression of Ehf rescues this phenotype

abnormal crypts of Lieberkuhn morphology ( J:268724 )

• short with fewer cells than in wild-type mice

abnormal small intestinal villus morphology ( J:268724 )

• short with fewer cells than in wild-type mice

abnormal enterocyte physiology ( J:268724 )

• impaired intestinal epithelium regeneration after radiation injury compared with wild-type mice

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:268724 )

• short with fewer cells than in wild-type mice

homeostasis/metabolism

abnormal response to radiation ( J:268724 )

• impaired intestinal epithelium regeneration after radiation injury compared with wild-type mice

Allelic Composition	Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ Stk26^tm2.1Zzh/Stk26^tm2.1Zzh
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Stk26^tm2.1Zzh mutation (0 available); any Stk26 mutation (11 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal enterocyte proliferation ( J:322311 )

• reduced proliferation of intestinal stem cells after 5-fluoruracil (5-FU)-induced killing of proliferating intestinal cells

digestive/alimentary system

abnormal enterocyte proliferation ( J:322311 )

• reduced proliferation of intestinal stem cells after 5-fluoruracil (5-FU)-induced killing of proliferating intestinal cells

Allelic Composition	Apc^tm2.1Cip/Apc⁺ Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apc^tm2.1Cip mutation (2 available); any Apc mutation (156 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

neoplasm

neoplasm ( J:186084 )

N	• following tamoxifen treatment, no intestinal tumors are observed

Allelic Composition	Cdc42^tm1Brak/Cdc42^tm1Brak Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc42^tm1Brak mutation (0 available); any Cdc42 mutation (44 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal crypts of Lieberkuhn morphology ( J:184563 )

• one week after tamoxifen administration, 56% of mutant crypts show stem cells forming a clustering pattern lacking clear demarcation between each other, while at 3 weeks after tamoxifen administration, 85% do so

• stem cells in mutant crypts 3 weeks after tamoxifen treatment lose the typical triangle shapes and marker analysis indicates disrupted crypt cell polarity

• loss of stem cells

• intestinal crypt cells, most likely Paneth cells, contain large vacuoles

absent Paneth cells ( J:184563 )

• reduction or absence of Paneth cell granules, indicating loss of Paneth cells

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:184563 )

• stem cells in mutant crypts 3 weeks after tamoxifen treatment lose the typical triangle shapes and marker analysis indicates disrupted crypt cell polarity

• loss of stem cells

• intestinal crypt cells, most likely Paneth cells, contain large vacuoles

absent Paneth cells ( J:184563 )

• reduction or absence of Paneth cell granules, indicating loss of Paneth cells

Allelic Composition	Elp3^tm1.1Tac/Elp3^tm1.1Tac Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NTac

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Elp3^tm1.1Tac mutation (0 available); any Elp3 mutation (40 available)
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

digestive/alimentary phenotype ( J:227334 )

N	• tamoxifen treated mice show normal numbers of Lgr5+ cells in intestinal crypts

Allelic Composition	Rprd1b^tm1Tshu/Rprd1b^tm1Tshu Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ Tg(Vil1-cre)20Syr/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Rprd1b^tm1Tshu mutation (0 available); any Rprd1b mutation (14 available)
Tg(Vil1-cre)20Syr mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal digestive system development ( J:300857 )

• decreased Lgr5-GFP+ cells pre and post tamoxifen treatment

• tamoxifen-treated cultured intestinal crypt organoids exhibit fewer outgrowths and fail to reform after disaggregation unlike control cultures

Allelic Composition	Rpap3^{tm1c(KOMP)Wtsi}/Rpap3^{tm1c(KOMP)Wtsi} Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
Genetic Background	involves: 129P2/OlaHsd * C57BL/6N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Rpap3^{tm1c(KOMP)Wtsi} mutation (0 available); any Rpap3 mutation (34 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:314346 )

• tamoxifen-treated mice are normal and survive for 3 weeks at least

Allelic Composition	Hmgcs2^tm1.1Yil/Hmgcs2^tm1.1Yil Lgr5^{tm3(cre/ERT2)Cle}/Lgr5⁺ Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺
Genetic Background	involves: 129S6/SvEvTac * C3H * C57BL/6J * C57BL/6NCrl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze} mutation (7 available); any Gt(ROSA)26Sor mutation (1095 available)
Hmgcs2^tm1.1Yil mutation (1 available); any Hmgcs2 mutation (33 available)
Lgr5^{tm3(cre/ERT2)Cle} mutation (0 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

abnormal jejunal goblet cell morphology ( J:280699 )

• 12 days after a single TAM injection, mice show a significant increase in the number of mucin+ goblet cells in proximal jejunal crypts

• TAM treatment followed administration of the HDAC inhibitor quisinostat (JNJ, 1 mg/kg, 5 i.p. injections) restores the number of AB/PAS+ goblet cells in proximal jejunal crypts

abnormal intestinal crypt stem cell physiology ( J:280699 )

• when mice are treated with TAM 24 h prior to crypt isolation, ISCs cultured in standard medium are 34.4% less capable of forming organoids, with resulting organoids showing a 40.5% increase in goblet cells and a 64.3% decrease in tdTomato+ cells per organoid relative to controls

• exogenous beta-hydroxybutyrate (beta-OHB), but not lactate (a Paneth niche-derived metabolite that sustains ISC function), restores the organoid-forming capacity and generation of tdTomato + clones and rescues the secretory lineage bias

increased Paneth cell number ( J:280699 )

• 12 and >22 days after a single TAM injection, mice show a significant increase in the number of LYZ1+ Paneth cells in proximal jejunal crypts

• 5 days after a single TAM injection, flow-sorted Lgr5+ ISC-derived tdTomato+ progeny show a 5.8-fold increase in Paneth cells (7.88% vs 1.36% in controls)

• as early 24 h after TAM injection, ISCs generate significantly greater numbers of tdTomato+ Paneth cells in jejunal sections

• TAM treatment followed administration of the HDAC inhibitor quisinostat (JNJ, 1 mg/kg, 5 i.p. injections) restores the number of LYZ+ Paneth cells in proximal jejunal crypts

abnormal jejunum crypts of Lieberkuhn morphology ( J:280699 )

• TAM-treated mice show a progressive loss of OLFM4+ intestinal stem cells (ISCs) and early progenitors (seen both at 10-12 days and at 17+ days but not at 5-7 days after a single TAM injection), along with an increase in the numbers of Paneth cells and goblet cells in proximal jejunal crypts

• 5 days after a single TAM injection, flow-sorted Lgr5+ ISC-derived tdTomato+ progeny show a modest increase in the fraction stem cells (35.34% vs 22.96% in controls), fewer transit-amplifying progenitors (18.40% vs 25.73% in controls) and a 5.8-fold increase in Paneth cells (7.88% vs 1.36% in controls), along with a weakened Lgr5+ stemness signature in ISCs but only minor effects on proliferation and apoptosis

• 12 days after a single TAM injection, crypt cells show induction of Atoh1 mRNA transcripts and reduction of Hes1 mRNA transcripts by ISH

• 17 days after a single TAM injection, no change in the proliferation or apoptosis of ISCs and progenitors is observed, and small intestine length, crypt depth, and numbers of chromogranin A+ enteroendocrine cells in jejunal crypts are normal

• TAM-treated mice show reduced numbers of H3K27ac-positive and Notch intracellular domain (NICD)-positive crypt cell nuclei, indicating increased class I histone deacetylase (HDAC) activity and less Notch signaling

• TAM treatment followed by administration of the HDAC inhibitor quisinostat (JNJ, 1 mg/kg, 5 i.p. injections) restores the number of H3K27ac-positive and NICD-positive crypt cell nuclei to wild-type numbers

enhanced intestine regeneration ( J:280699 )

• when mice are fed a ketogenic diet (KTD) for 4 weeks, TAM-treated and then irradiated, the number of tdTomato+ crypts per mm jejunum is significantly higher than in chow-fed control mice, indicating enhanced regenerative output of tdTomato-labeled ISCs after injury

impaired intestine regeneration ( J:280699 )

• when mice are treated with TAM 1 day prior to radiation-induced intestinal epithelial injury, ISCs generate 5-fold less labeled tdTomato+ crypts with fewer Lgr5+ ISC-derived labeled progeny extending up crypt-villous units at 5 days post-radiation, and the overall number of surviving intact jejunal crypts is reduced by 2-fold

• oral administration of poly(lactic-co-glycolic acid) (PLGA) encapsulated beta-hydroxybutyrate (beta-OHB) nanoparticles or betaOHB oligomers partially rescues intestinal regeneration after radiation-induced damage

• TAM treatment followed administration of the HDAC inhibitor quisinostat (JNJ, 1 mg/kg, 5 i.p. injections) rescues the decline in ISC numbers and ISC function after radiation-induced injury

endocrine/exocrine glands

increased Paneth cell number ( J:280699 )

• 12 and >22 days after a single TAM injection, mice show a significant increase in the number of LYZ1+ Paneth cells in proximal jejunal crypts

• 5 days after a single TAM injection, flow-sorted Lgr5+ ISC-derived tdTomato+ progeny show a 5.8-fold increase in Paneth cells (7.88% vs 1.36% in controls)

• as early 24 h after TAM injection, ISCs generate significantly greater numbers of tdTomato+ Paneth cells in jejunal sections

• TAM treatment followed administration of the HDAC inhibitor quisinostat (JNJ, 1 mg/kg, 5 i.p. injections) restores the number of LYZ+ Paneth cells in proximal jejunal crypts

abnormal jejunum crypts of Lieberkuhn morphology ( J:280699 )

• 12 days after a single TAM injection, crypt cells show induction of Atoh1 mRNA transcripts and reduction of Hes1 mRNA transcripts by ISH

homeostasis/metabolism

abnormal response to radiation ( J:280699 )

• mice treated with TAM 1 day prior to radiation-induced intestinal epithelial injury show impaired Lgr5+ ISC-mediated repair in jejunal crypts relative to controls

• oral administration of nanoparticle PLGA-encapsulated beta-OHB or betaOHB oligomers partially rescues intestinal regeneration after radiation-induced damage

• when mice are fed a ketogenic diet (KTD) for 4 weeks, TAM-treated and then irradiated, the number of tdTomato+ crypts per mm jejunum is significantly higher than in chow-fed controls, indicating enhanced regenerative output of tdTomato-labeled ISCs after injury

• TAM treatment followed administration of the HDAC inhibitor quisinostat (JNJ, 1 mg/kg, 5 i.p. injections) rescues the decline in ISC numbers and ISC function after radiation-induced injury

cellular

abnormal jejunal goblet cell morphology ( J:280699 )

• 12 days after a single TAM injection, mice show a significant increase in the number of mucin+ goblet cells in proximal jejunal crypts

• TAM treatment followed administration of the HDAC inhibitor quisinostat (JNJ, 1 mg/kg, 5 i.p. injections) restores the number of AB/PAS+ goblet cells in proximal jejunal crypts

abnormal intestinal crypt stem cell physiology ( J:280699 )

Allelic Composition	Lgr5^{tm3(cre/ERT2)Cle}/Lgr5⁺ Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺
Genetic Background	involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze} mutation (7 available); any Gt(ROSA)26Sor mutation (1095 available)
Lgr5^{tm3(cre/ERT2)Cle} mutation (0 available); any Lgr5 mutation (58 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

digestive/alimentary system

impaired intestine regeneration ( J:280699 )

• when mice are fed with a glucose-supplemented chow diet for 2 weeks and treated with TAM prior to radiation-induced intestinal injury, the ability of ISCs to generate tdTomato+ labeled progeny is reduced by 2-fold while the overall number of surviving intact jejunal crypts is significantly decreased at 3 days post-radiation

• a single oral bolus of beta-hydroxybutyrate (beta-OHB) 16 h prior to irradiation rescues these functional deficits

homeostasis/metabolism

abnormal response to radiation ( J:280699 )

• when mice are fed with a glucose-supplemented chow diet for 2 weeks and treated with TAM prior to radiation-induced intestinal injury, the ability of intestinal stem cells (ISCs) to generate tdTomato+ labeled progeny is reduced by 2-fold while the overall number of surviving intact jejunal crypts is significantly decreased at 3 days post-radiation

• a single oral bolus of beta-hydroxybutyrate (beta-OHB) 16 h prior to irradiation rescues these functional deficits

Allelic Composition	Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1^{tm1.1(cre/ERT2)Rjc}
Genetic Background	involves: 129 * 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Lrig1^{tm1.1(cre/ERT2)Rjc} mutation (1 available); any Lrig1 mutation (49 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

neoplasm

increased intestinal adenoma incidence ( J:186084 )

• at 5-6 months, nearly 90% of mice develop duodenal tumors (usually low grade adenomas)

• in one instance, a 13-month old mouse had a tumor with areas showing high grade dysplasia, and focal extension of neoplastic glands into deeper layers of the bowel wall; tumor showed intact Apc however

digestive/alimentary system

abnormal crypts of Lieberkuhn morphology ( J:186084 )

• number of crypts showing Lgr5-EGFP fluorescence is doubled in absence of Lrig1 protein, compared to mice with one or two copies of Lrig1

increased intestinal adenoma incidence ( J:186084 )

• at 5-6 months, nearly 90% of mice develop duodenal tumors (usually low grade adenomas)

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:186084 )

• number of crypts showing Lgr5-EGFP fluorescence is doubled in absence of Lrig1 protein, compared to mice with one or two copies of Lrig1

Allelic Composition	Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ Mex3a^{tm1.1(KOMP)Vlcg}/Mex3a⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * C57BL/6NTac

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lgr5^{tm1(cre/ERT2)Cle} mutation (1 available); any Lgr5 mutation (58 available)
Mex3a^{tm1.1(KOMP)Vlcg} mutation (0 available); any Mex3a mutation (27 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:287423 )

• 16% of mice exhibit postnatal lethality associated with disturbances in the intestinal epithelium

digestive/alimentary system

abnormal intestinal epithelium morphology ( J:287423 )

• disturbances in the intestinal epithelium

abnormal small intestine crypts of Lieberkuhn morphology ( J:287423 )

• loss of Lgr5+ intestinal stem cells in the intestine

endocrine/exocrine glands

abnormal small intestine crypts of Lieberkuhn morphology ( J:287423 )

• loss of Lgr5+ intestinal stem cells in the intestine

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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