Stk26^tm2.1Zzh
Targeted Allele Detail

Summary

• Symbol: Stk26^tm2.1Zzh
• Name: serine/threonine kinase 26; targeted mutation 2.1, Zhaocai Zhou
• MGI ID: MGI:7490341
• Synonyms: Mst4^T178E
• Gene: Stk26 Location: ChrX:49930052-49981980 bp, + strand Genetic Position: ChrX, 27.5 cM, cytoband A3.3
• Alliance: Stk26^tm2.1Zzh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:322311
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site (forward orientation) was inserted into intron 4 and a lox2272 site (forward orientation), an inverted copy of exons 5-6 (including intervening intron) where exon 6 contains a p.T178E mutation, a second loxP site (reverse orientation), a second lox2272 site (reverse orientation) and an FRT site flanked neomycin resistance gene were inserted into intron 6. The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses the wild-type transcript and peptide. Only after Cre-mediated recombination between the loxP and lox2272 sites will the inserted exons be flipped and replace the endogenous exons, allowing expression of the mutated peptide. The mutation involves changing threonine codon 178 (ACC) in exon 6 to glutamic acid (GAG), which is a phosphomimic and confers constitutive kinase activity upon the encoded peptide. (J:322311)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stk26 Mutation: 15 strains or lines available

References

• Original: J:322311 Zhang H, et al., An MST4-pbeta-Catenin(Thr40) Signaling Axis Controls Intestinal Stem Cell and Tumorigenesis. Adv Sci (Weinh). 2021 Sep;8(17):e2004850
• All: 1 reference(s)