Phenotypes associated with this allele

• Allele Symbol: Itgb3^tm1Hyn
• Allele Name: targeted mutation 1, Richard Hynes
• Allele ID: MGI:2175913
• Summary: 12 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Itgb3^tm1Hyn/Itgb3^tm1Hyn	involves: 129S2/SvPas	MGI:4438877
hm2	Itgb3^tm1Hyn/Itgb3^tm1Hyn	involves: 129S2/SvPas * BALB/c	MGI:4888413
hm3	Itgb3^tm1Hyn/Itgb3^tm1Hyn	involves: 129S2/SvPas * C57BL/6	MGI:2177829
ht4	Itgb3^tm1Hyn/Itgb3^+	involves: 129S2/SvPas * C57BL/6	MGI:4439371
cn5	Itgb3^tm1Hyn/Itgb3^tm1.1Wlbcr Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:5688877
cn6	Itgb3^tm1Hyn/Itgb3^tm1.1Wlbcr Tg(Pf4-icre)Q3Rsko/0	involves: 129S2/SvPas * C57BL/6	MGI:5688878
cx7	Apoe^tm1Unc/Apoe^tm1Unc Itgb3^tm1Hyn/Itgb3^tm1Hyn	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:4439279
cx8	Fbn1^tm3.1Hcd/Fbn1^+ Itgb3^tm1Hyn/Itgb3^+	involves: 129S * 129X1/SvJ * C57BL/6J * FVB/N	MGI:5558883
cx9	Fbn1^tm2.1Hcd/Fbn1^+ Itgb3^tm1Hyn/Itgb3^+	involves: 129S * 129X1/SvJ * C57BL/6J * FVB/N	MGI:5558881
cx10	Itgb3^tm1Hyn/Itgb3^tm1Hyn Itgb5^tm1Des/Itgb5^tm1Des	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6	MGI:4438938
cx11	Itgb3^tm1Hyn/Itgb3^tm1Hyn Ldlr^tm1Her/Ldlr^tm1Her	involves: 129S2/SvPas * 129S7/SvEvBrd * C57BL/6	MGI:4439281
cx12	Ilk^tm2Star/Ilk^+ Itgb3^tm1Hyn/Itgb3^+	involves: 129S2/SvPas * C57BL/6	MGI:5502226

Genotype
MGI:4438877

hm1

• Allelic Composition: Itgb3^tm1Hyn/Itgb3^tm1Hyn
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

abnormal osteoclast morphology ( J:60672 )

• the ruffled membrane is thick with blunted projections

• actin rings fail to form and actin is diffusely distributed, in a punctate pattern

increased osteoclast cell number ( J:60672 )

• about a 3 fold increase per mm trabecular bone surface

abnormal osteoclast physiology ( J:60672 )

• bone marrow macrophages differentiate into osteoclasts that fail to spread

increased compact bone thickness ( J:60672 )

• increased bone mass

increased trabecular bone thickness ( J:60672 )

• decreased ability of bone marrow macrophage derived osteoclasts to excavate whale dentin and produce well defined resorption lacunae

osteosclerosis ( J:60672 )

• increased bone mass

hematopoietic system

decreased macrophage cell number ( J:96049 )

• number of macrophage in wounds is significantly lower than in wild-type wounds

abnormal osteoclast morphology ( J:60672 )

• the ruffled membrane is thick with blunted projections

• actin rings fail to form and actin is diffusely distributed, in a punctate pattern

increased osteoclast cell number ( J:60672 )

• about a 3 fold increase per mm trabecular bone surface

abnormal osteoclast physiology ( J:60672 )

• bone marrow macrophages differentiate into osteoclasts that fail to spread

abnormal platelet physiology ( J:96049 )

• platelets release twofold more TGF

immune system

decreased macrophage cell number ( J:96049 )

• number of macrophage in wounds is significantly lower than in wild-type wounds

abnormal osteoclast morphology ( J:60672 )

• the ruffled membrane is thick with blunted projections

• actin rings fail to form and actin is diffusely distributed, in a punctate pattern

increased osteoclast cell number ( J:60672 )

• about a 3 fold increase per mm trabecular bone surface

abnormal osteoclast physiology ( J:60672 )

• bone marrow macrophages differentiate into osteoclasts that fail to spread

homeostasis/metabolism

abnormal platelet physiology ( J:96049 )

• platelets release twofold more TGF

decreased circulating calcium level ( J:60672 )

increased transforming growth factor beta level ( J:96049 )

• platelets release twofold more TGF

enhanced wound healing ( J:96049 )

• re-epithelialization is accelerated significantly

• dermal fibroblast recruitment in wounded tissue is significantly elevated with enhanced fibronectin and vitronectin deposition in granulation tissue

cardiovascular system

abnormal physiological neovascularization ( J:96049 )

• increase in vascularization in wounds after 7 days of healing

integument

decreased keratinocyte proliferation ( J:96049 )

• in the hyperproliferative epidermal region 3 days after wounding

cellular

decreased keratinocyte proliferation ( J:96049 )

• in the hyperproliferative epidermal region 3 days after wounding

Genotype
MGI:4888413

hm2

• Allelic Composition: Itgb3^tm1Hyn/Itgb3^tm1Hyn
• Genetic Background: involves: 129S2/SvPas * BALB/c

cellular

maternal effect ( J:131241 )

• pups and fetuses from homozygous females immunized two or four times with wild-type platelets, respectively, and mated with wild-type males exhibit hemorrhaging, reduced platelets and increased mortality, a phenotype similar to human fetal and neonatal alloimmune thrombocytopenia

• heterozygous pups from non-immunized females mated with wild-type males exhibit normal platelets counts, indicating that maternal antibodies of the pregnant females and not pup genotype is responsible for the reduced platelets and hemorrhaging in heterozygotes

mortality/aging

pregnancy-related premature death ( J:131241 )

• one of three homozygous females immunized four times with wild-type platelets and mated with a wild-type male, died during delivery after a 3-week pregnancy

reproductive system

increased miscarriage rate ( J:131241 )

♀ • homozygous females immunized with wild-type platelets and mated with wild-type males, exhibit miscarriages due to internal organ bleeding of fetuses; females immunized 4 times show more miscarriages than females immunized 2 times with wild-type platelets

immune system

immune system phenotype ( J:131241 )

N • both T-helper 1 and T-helper 2 immune responses to platelet Itgb3 antigen exist in mutants

increased IgG level ( J:131241 )

• titer of maternal IgG against Itgb3 in homozygous females increases with the number of times females are immunized with wild-type platelets

• however, homozygous preimmunized females do not show any detectable anti-Itgb3 IgG after the first and second deliveries after mating with wild-type males; low levels of anti-Itgb3 antibodies are seen in the third and subsequent deliveries

hematopoietic system

increased IgG level ( J:131241 )

• titer of maternal IgG against Itgb3 in homozygous females increases with the number of times females are immunized with wild-type platelets

• however, homozygous preimmunized females do not show any detectable anti-Itgb3 IgG after the first and second deliveries after mating with wild-type males; low levels of anti-Itgb3 antibodies are seen in the third and subsequent deliveries

Genotype
MGI:2177829

hm3

• Allelic Composition: Itgb3^tm1Hyn/Itgb3^tm1Hyn
• Genetic Background: involves: 129S2/SvPas * C57BL/6

Survival of Itgb3^tm1Hyn/Itgb3^tm1Hyn mice is compromised due to placental defects and hemorrhage

growth/size/body

increased heart weight ( J:119666 )

• heart weight to body weight ratio is increased

cardiac hypertrophy ( J:119666 )

• in 12-week-old spontaneous cardiac hypertrophy occurs in the absence of provocative stimulation

heart left ventricle hypertrophy ( J:119666 )

enlarged spleen ( J:52262 )

• commonly associated with gastrointestinal bleeding

mortality/aging

premature death ( J:52262 )

• some homozygous animals suffered acute blood loss and crisis which lead to premature death

postnatal lethality, incomplete penetrance ( J:52262 )

• significant loss of homozygous pups up to three weeks of age due to hemorrhage

cardiovascular system

abnormal induced retina neovascularization ( J:73752 )

• hypoxia-induced retinal neovascularization is significantly elevated as measure by numbers of vascular glomeruli at P17

abnormal tumor vascularization ( J:73752 )

• significantly elevated numbers of vessels per square millimeter of tumors, from injected cells, when compared with wild-type controls

increased angiogenesis ( J:73752 )

• blood-vessel infiltration of matrigel implants impregnated with VEGF is significantly elevated

• in the presence of VEGF, the total number of vessel sprouts in aortic rings embedded in Matrigel is significantly higher compared to wild-type

• however, no increase in vessel density is seen on scruff skin

increased heart weight ( J:119666 )

• heart weight to body weight ratio is increased

cardiac hypertrophy ( J:119666 )

• in 12-week-old spontaneous cardiac hypertrophy occurs in the absence of provocative stimulation

heart left ventricle hypertrophy ( J:119666 )

increased heart left ventricle weight ( J:119666 )

• left ventricle weight to body weight ratio is increased by 4 weeks of age

hemorrhage ( J:52262 )

• some homozygous embryos and newborn pups displayed hemorrhage in the skin, in petechiae and in purpura, and appeared anemic

uterine hemorrhage ( J:52262 )

♀

gastrointestinal hemorrhage ( J:52262 )

• in some homozygous newborn pups which die before weaning and in many of the surviving homozygous adult mice

skin hemorrhage ( J:52262 )

decreased cardiac muscle contractility ( J:119666 )

• a significantly impaired contractile response to infusion of the inotropic agent Dobutamine

abnormal cardiac muscle relaxation ( J:119666 )

• diastolic relaxation is impaired (dP/dt min and tau (Glantz) are significantly increased)

decreased left ventricle systolic pressure ( J:119666 )

• 12-week-old mice have reduced systolic function compared to wild-type littermates

• left ventricular peak systolic pressure is significantly decreased 7 days after pressure overload by transverse aortic constriction surgery (TAC) compared to wild-type

• left ventricular peak systolic pressure is decreased in 12-week-old mice

increased left ventricle diastolic pressure ( J:119666 )

• left ventricular end-diastolic pressure is significantly increased 7 days after pressure overload by transverse aortic constriction surgery (TAC) compared to wild-type

• the maximal change in pressure per unit time is significantly decreased 7 days after pressure overload by TAC surgery compared to wild-type

• minimal change in pressure per unit time is significantly increased 7 days after pressure overload by TAC surgery compared to wild-type

decreased heart rate ( J:119666 )

• heart rate is significantly decreased 7 days after pressure overload by transverse aortic constriction surgery (TAC) compared to wild-type

increased response of heart to induced stress ( J:119666 )

• impairment of systolic and diastolic function after transverse aortic constriction is more severe than in wild-type controls

• cardiac tissue exhibits marked round cell infiltration 7 days after transverse aortic constriction surgery

hematopoietic system

enlarged spleen ( J:52262 )

• commonly associated with gastrointestinal bleeding

extramedullary hematopoiesis ( J:52262 )

• found in animals with splenomegaly

anemia ( J:52262 )

• secondary to the excessive bleeding

decreased erythrocyte cell number ( J:52262 )

• in 8-12-week-old mice

decreased hematocrit ( J:52262 )

• in 8-12-week-old mice

decreased hemoglobin content ( J:52262 )

• in 8-12-week-old mice

increased monocyte cell number ( J:119666 )

• the monocyte count is dramatically increased in peripheral blood

reticulocytosis ( J:52262 )

• in 8-12-week-old mice

abnormal macrophage physiology ( J:119666 )

• peritoneal macrophages express significantly more Tnf and Il1b

impaired macrophage chemotaxis ( J:119666 )

• peritoneal macrophages migrate less efficiently

abnormal platelet activation ( J:52262 )

• reduced formation of thrombi in Shwartzman-like reaction, an experimental model of hemorrhagic vasculitis induced by subcutaneous injection of LPS followed 24 h later by an injection of TNF-alpha in the same place

• platelet isolated from homozygous mice did not aggregate after ADP-induced activation in vitro

• platelet isolated from homozygous mice shows reduced clot retraction and greatly reduced fibrinogen uptake in vitro

reproductive system

uterine hemorrhage ( J:52262 )

♀

increased miscarriage rate ( J:52262 )

♀ • 9.5% of the embryos carried by homozygous mothers were found dead in utero at E14 and E17

abnormal uterine environment ( J:52262 )

♀ • compromised survival of embryos in homozygous mother as a result of placental defects and intrauterine hemorrhage

reduced female fertility ( J:52262 )

♀ • litter sizes of homozygous female were reduced significantly compared to wild-type

embryo

abnormal trophoblast giant cell morphology ( J:52262 )

• within affected placentae of homozygous pregnant females, trophoblastic cells sometimes invaded the labyrinthine part of the placenta

abnormal placenta labyrinth morphology ( J:52262 )

• thickened cell layers in the labyrinth layer in approximately 25% of placentae of homozygous pregnant females which were associated with dead embryos

• within affected placentae, the space between endothelial layers was reduced with evidence of increased necrosis

pale placenta ( J:52262 )

digestive/alimentary system

gastrointestinal hemorrhage ( J:52262 )

• in some homozygous newborn pups which die before weaning and in many of the surviving homozygous adult mice

immune system

enlarged spleen ( J:52262 )

• commonly associated with gastrointestinal bleeding

increased monocyte cell number ( J:119666 )

• the monocyte count is dramatically increased in peripheral blood

abnormal macrophage physiology ( J:119666 )

• peritoneal macrophages express significantly more Tnf and Il1b

impaired macrophage chemotaxis ( J:119666 )

• peritoneal macrophages migrate less efficiently

myositis ( J:119666 )

• cardiac tissue exhibits marked round cell infiltration 7 days after transverse aortic constriction surgery

dermatitis ( J:52262 )

• ulcerative dermatitis in some homozygous animals undergoing crisis

homeostasis/metabolism

increased response of heart to induced stress ( J:119666 )

• impairment of systolic and diastolic function after transverse aortic constriction is more severe than in wild-type controls

• cardiac tissue exhibits marked round cell infiltration 7 days after transverse aortic constriction surgery

abnormal platelet activation ( J:52262 )

• reduced formation of thrombi in Shwartzman-like reaction, an experimental model of hemorrhagic vasculitis induced by subcutaneous injection of LPS followed 24 h later by an injection of TNF-alpha in the same place

• platelet isolated from homozygous mice did not aggregate after ADP-induced activation in vitro

• platelet isolated from homozygous mice shows reduced clot retraction and greatly reduced fibrinogen uptake in vitro

increased bleeding time ( J:52262 )

• impaired hemostasis as evidenced by failure to arrest bleeding within 10 minutes following tail resection

renal/urinary system

renal necrosis ( J:52262 )

• enlarged glomeruli and dilated and necrotic proximal tubules containing proteinaceous deposits are found secondary to the excessive bleeding

liver/biliary system

hepatic necrosis ( J:52262 )

• centrilobular necrosis of the liver are found secondary to the excessive bleeding

vision/eye

vision/eye phenotype ( J:52262 )

N • the patterns of neovascularization in P7 retinas isolated from wild-type or homozygous mutant mice showed no obvious differences

abnormal induced retina neovascularization ( J:73752 )

• hypoxia-induced retinal neovascularization is significantly elevated as measure by numbers of vascular glomeruli at P17

neoplasm

abnormal tumor vascularization ( J:73752 )

• significantly elevated numbers of vessels per square millimeter of tumors, from injected cells, when compared with wild-type controls

increased tumor growth/size ( J:73752 )

• tumor size is enhanced significantly when injected with murine melanoma (B16F0) or lung carcinoma (CMT19T) cells

cellular

abnormal cell adhesion ( J:73752 )

• endothelial cells showed background levels of adhesion and no migration on vitronectin

hepatic necrosis ( J:52262 )

• centrilobular necrosis of the liver are found secondary to the excessive bleeding

renal necrosis ( J:52262 )

• enlarged glomeruli and dilated and necrotic proximal tubules containing proteinaceous deposits are found secondary to the excessive bleeding

abnormal cell migration ( J:73752 )

• endothelial cells showed background levels of adhesion and no migration on vitronectin

impaired macrophage chemotaxis ( J:119666 )

• peritoneal macrophages migrate less efficiently

muscle

heart left ventricle hypertrophy ( J:119666 )

decreased cardiac muscle contractility ( J:119666 )

• a significantly impaired contractile response to infusion of the inotropic agent Dobutamine

abnormal cardiac muscle relaxation ( J:119666 )

• diastolic relaxation is impaired (dP/dt min and tau (Glantz) are significantly increased)

myositis ( J:119666 )

• cardiac tissue exhibits marked round cell infiltration 7 days after transverse aortic constriction surgery

integument

skin hemorrhage ( J:52262 )

dermatitis ( J:52262 )

• ulcerative dermatitis in some homozygous animals undergoing crisis

petechiae ( J:52262 )

• petechiae are observed around the mouth, indicating bleeding under the epidermis

purpura ( J:52262 )

• purpura are observed on the forelimb, indicating bleeding within the dermis

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
platelet-type bleeding disorder 16		DOID:0060691	OMIM:187800	J:52262

Genotype
MGI:4439371

ht4

• Allelic Composition: Itgb3^tm1Hyn/Itgb3⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6

cardiovascular system

increased heart weight ( J:119666 )

• heart weight to body weight ratio is increased

cardiac hypertrophy ( J:119666 )

• the hypertrophy phenotype is intermediate between homozygous null and wild-type mice

heart left ventricle hypertrophy ( J:119666 )

• spontaneous

increased heart left ventricle weight ( J:119666 )

• left ventricle weight to body weight ratio is increased

decreased heart rate ( J:119666 )

• heart rate is significantly decreased 7 days after pressure overload by transverse aortic constriction surgery (TAC) compared to wild-type

increased response of heart to induced stress ( J:119666 )

• impairment of systolic and diastolic function after transverse aortic constriction is more severe than in wild-type controls

• heart rate is significantly decreased 7 days after pressure overload by transverse aortic constriction surgery (TAC) compared to wild-type

homeostasis/metabolism

increased response of heart to induced stress ( J:119666 )

• impairment of systolic and diastolic function after transverse aortic constriction is more severe than in wild-type controls

• heart rate is significantly decreased 7 days after pressure overload by transverse aortic constriction surgery (TAC) compared to wild-type

growth/size/body

increased heart weight ( J:119666 )

• heart weight to body weight ratio is increased

cardiac hypertrophy ( J:119666 )

• the hypertrophy phenotype is intermediate between homozygous null and wild-type mice

heart left ventricle hypertrophy ( J:119666 )

• spontaneous

muscle

heart left ventricle hypertrophy ( J:119666 )

• spontaneous

Genotype
MGI:5688877

cn5

• Allelic Composition: Itgb3^tm1Hyn/Itgb3^tm1.1Wlbcr; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

cellular

abnormal osteoclast differentiation ( J:224675 )

• osteoclasts exhibit decreased fusion during differentiation

hematopoietic system

abnormal osteoclast morphology ( J:224675 )

• decreased size of multinucleate osteoclasts as compared to controls

• osteoclasts exhibit decreased fusion during differentiation

abnormal osteoclast differentiation ( J:224675 )

• osteoclasts exhibit decreased fusion during differentiation

decreased osteoclast cell number ( J:224675 )

• numbers of osteoclasts are decreased during differentiation

homeostasis/metabolism

decreased collagen level ( J:224675 )

• decreased levels of serum type I collagen as compared to wild type

immune system

abnormal osteoclast morphology ( J:224675 )

• decreased size of multinucleate osteoclasts as compared to controls

• osteoclasts exhibit decreased fusion during differentiation

abnormal osteoclast differentiation ( J:224675 )

• osteoclasts exhibit decreased fusion during differentiation

decreased osteoclast cell number ( J:224675 )

• numbers of osteoclasts are decreased during differentiation

skeleton

abnormal osteoclast morphology ( J:224675 )

• decreased size of multinucleate osteoclasts as compared to controls

• osteoclasts exhibit decreased fusion during differentiation

abnormal osteoclast differentiation ( J:224675 )

• osteoclasts exhibit decreased fusion during differentiation

decreased osteoclast cell number ( J:224675 )

• numbers of osteoclasts are decreased during differentiation

increased bone mineral density ( J:224675 )

• increased bone mineral density is found in 2 and 6 month old mice as compared to controls

increased trabecular bone volume ( J:224675 )

• increased trabecular bone volume in femoral and tibial bones

osteopetrosis ( J:224675 )

neoplasm

abnormal tumor pathology ( J:224675 )

• macrophage infiltration is decreased in implanted subcutaneous tumors as compared to tumor implanted controls

abnormal tumor susceptibility ( J:224675 )

• implanted melanoma cells have an increased live cell mass and volume as compared to tumor implanted controls

Genotype
MGI:5688878

cn6

• Allelic Composition: Itgb3^tm1Hyn/Itgb3^tm1.1Wlbcr; Tg(Pf4-icre)Q3Rsko/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6

mortality/aging

postnatal lethality, incomplete penetrance ( J:224675 )

• 15% of mice die postnatally due to spontaneous bleeding

digestive/alimentary system

gastrointestinal hemorrhage ( J:224675 )

• 85% of 8-13 week old mice exhibit gastrointestinal bleeding

hematopoietic system

enlarged spleen ( J:224675 )

decreased hemoglobin content ( J:224675 )

• decreased hemoglobin levels

decreased platelet aggregation ( J:224675 )

• platelet-rich plasma (PRP) does not undergo ADP-induced platelet aggregation

homeostasis/metabolism

abnormal hemostasis ( J:224675 )

abnormal blood coagulation ( J:224675 )

• clot retraction does not occur in platelet-rich plasma (PRP)

decreased platelet aggregation ( J:224675 )

• platelet-rich plasma (PRP) does not undergo ADP-induced platelet aggregation

increased bleeding time ( J:224675 )

immune system

enlarged spleen ( J:224675 )

cardiovascular system

gastrointestinal hemorrhage ( J:224675 )

• 85% of 8-13 week old mice exhibit gastrointestinal bleeding

intracranial hemorrhage ( J:224675 )

• acute and chronic intracranial bleeding

nervous system

intracranial hemorrhage ( J:224675 )

• acute and chronic intracranial bleeding

growth/size/body

enlarged spleen ( J:224675 )

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Glanzmann's thrombasthenia		DOID:2219	OMIM:273800	J:224675

Genotype
MGI:4439279

cx7

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Itgb3^tm1Hyn/Itgb3^tm1Hyn
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:83615 )

• at 16 weeks of age after 10 weeks on a western style diet only 52% of mice compared to 100% survival for diet matched Ldlr single mutants

• no increase in mortality is seen in mice kept on a standard chow diet

premature death ( J:83615 )

• at 12 weeks of age after 6 weeks on a western style diet, survival is reduced to 38% for mice compared to 96% for diet matched Apoe single mutants

postnatal lethality, incomplete penetrance ( J:83615 )

• similar to that in Itgb3 single mutants

• only 62% survival to weaning for compared to 96% survival in mice null for Apoe alone

immune system

lung inflammation ( J:83615 )

• isolated small foci of mononuclear cells are present in lung after 6 weeks of high-fat feeding

• in mice fed a high fat diet pneumonitis is the cause of death

respiratory system

lung inflammation ( J:83615 )

• isolated small foci of mononuclear cells are present in lung after 6 weeks of high-fat feeding

• in mice fed a high fat diet pneumonitis is the cause of death

hematopoietic system

anemia ( J:83615 )

• western diet fed mice are anemic compared to diet matched mice null for Apoe alone

growth/size/body

decreased susceptibility to diet-induced obesity ( J:83615 )

• after 6 weeks on a western diet mice weighed less than diet matched Apoe single mutants

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:83615 )

• after 6 weeks on a western diet mice weighed less than diet matched Apoe single mutants

cardiovascular system

atherosclerotic lesions ( J:83615 )

• on a western diet, lesions are 2.6 fold greater at the arch, 3.3 fold greater at the thoracic aorta, and 5.6-fold greater at the abdominal aorta compared to diet matched Apoe single mutants

• on chow diet at approximately 11 months of age, lesions are 2.4 fold greater at the arch, 3 fold greater at the thoracic aorta than in diet matched Apoe single mutants.

Genotype
MGI:5558883

cx8

• Allelic Composition: Fbn1^tm3.1Hcd/Fbn1⁺; Itgb3^tm1Hyn/Itgb3⁺
• Genetic Background: involves: 129S * 129X1/SvJ * C57BL/6J * FVB/N

integument

integument phenotype ( J:206074 )

N • skin stiffness and collagen deposition are normalized at 3 months

abnormal cutaneous collagen fibril morphology ( J:206074 )

• increased collagen in the dermis of some mice at 5 months as in Itgb3-deficient mice

thick dermal layer ( J:206074 )

• in some mice at 5 months as in Itgb3-deficient mice

epidermal hyperplasia ( J:206074 )

• focal in some mice at 5 months as in Itgb3-deficient mice

Genotype
MGI:5558881

cx9

• Allelic Composition: Fbn1^tm2.1Hcd/Fbn1⁺; Itgb3^tm1Hyn/Itgb3⁺
• Genetic Background: involves: 129S * 129X1/SvJ * C57BL/6J * FVB/N

integument

integument phenotype ( J:206074 )

N • skin stiffness and collagen deposition are normalized at 3 months

abnormal cutaneous collagen fibril morphology ( J:206074 )

• increased collagen in the dermis of some mice at 5 months as in Itgb3-deficient mice

thick dermal layer ( J:206074 )

• in some mice at 5 months as in Itgb3-deficient mice

epidermal hyperplasia ( J:206074 )

• focal in some mice at 5 months as in Itgb3-deficient mice

Genotype
MGI:4438938

cx10

• Allelic Composition: Itgb3^tm1Hyn/Itgb3^tm1Hyn; Itgb5^tm1Des/Itgb5^tm1Des
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * C57BL/6

neoplasm

abnormal tumor vascularization ( J:73752 )

• cell line derived tumors are significantly hyper-vascularized when compared with controls; however, vascular density in normal skin is not significantly different from controls

increased tumor growth/size ( J:73752 )

• tumor size is enhanced significantly when injected with murine melanoma (B16F0) or lung carcinoma (CMT19T) cells

cardiovascular system

abnormal tumor vascularization ( J:73752 )

• cell line derived tumors are significantly hyper-vascularized when compared with controls; however, vascular density in normal skin is not significantly different from controls

Genotype
MGI:4439281

cx11

• Allelic Composition: Itgb3^tm1Hyn/Itgb3^tm1Hyn; Ldlr^tm1Her/Ldlr^tm1Her
• Genetic Background: involves: 129S2/SvPas * 129S7/SvEvBrd * C57BL/6

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:83615 )

• at 16 weeks of age after 10 weeks on a western style diet only 52% of mice survive compared to 100% survival for diet matched Ldlr single mutants

• no increase in mortality is seen in mice kept on a standard chow diet

decreased survivor rate ( J:83615 )

• at 16 weeks of age after 10 weeks on a western style diet only 52% of mice survive compared to 100% survival for diet matched Ldlr single mutants

homeostasis/metabolism

increased circulating glucose level ( J:83615 )

• the level after 10 weeks on a western diet is higher than in diet matched Ldlr single mutants

decreased circulating cholesterol level ( J:83615 )

• cholesterol levels after 6 and 10 weeks on a western diet are lower than in diet matched Ldlr single mutants

cardiovascular system

atherosclerotic lesions ( J:83615 )

• on a western style diet, lesions are 2.0 fold greater at the arch and 4.1 fold greater at the thoracic aorta compared to diet matched Ldlr single mutants

Genotype
MGI:5502226

cx12

• Allelic Composition: Ilk^tm2Star/Ilk⁺; Itgb3^tm1Hyn/Itgb3⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6

skeleton

increased trabecular bone thickness ( J:197593 )

• mice display significant increase in thickness of the trabeculae at the primary spongiosa