Itgb3^tm1.1Wlbcr
Targeted Allele Detail

Summary

• Symbol: Itgb3^tm1.1Wlbcr
• Name: integrin beta 3; targeted mutation 1.1, Katherine Weilbaecher
• MGI ID: MGI:5688572
• Synonyms: B3^flox, beta3^flox
• Gene: Itgb3 Location: Chr11:104498826-104561302 bp, + strand Genetic Position: Chr11, 67.84 cM
• Alliance: Itgb3^tm1.1Wlbcr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224675
• Parent Cell Line: B6/BLU (ES Cell)
• Strain of Origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The targeting vector was designed to insert a single loxP site upstream of exon 1 and loxP-flanked PGK-neomycin resistance (neo) cassette downstream of exon 1. The neo cassette was deleted by transiently transfection of a pTURBO-Cre expression plasmid leaving exon 1 floxed. (J:224675)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Itgb3 Mutation: 43 strains or lines available

Notes

B6/BLU embryonic stem cells contain a LacZ reporter fused to beta-globin and is expressed in peripheral blood cells.

References

• Original: J:224675 Morgan EA, et al., Dissection of platelet and myeloid cell defects by conditional targeting of the beta3-integrin subunit. FASEB J. 2010 Apr;24(4):1117-27
• All: 12 reference(s)