Phenotypes associated with this allele

• Allele Symbol: Pik3cg^tm1Pngr
• Allele Name: targeted mutation 1, Josef M Penninger
• Allele ID: MGI:1858013
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pik3cg^tm1Pngr/Pik3cg^tm1Pngr	B6.129P2-Pik3cg^tm1Pngr	MGI:3796517
hm2	Pik3cg^tm1Pngr/Pik3cg^tm1Pngr	involves: 129P2/OlaHsd	MGI:3619931
hm3	Pik3cg^tm1Pngr/Pik3cg^tm1Pngr	involves: 129P2/OlaHsd * C57BL/6	MGI:3619226
cn4	Pik3cg^tm1Pngr/Pik3cg^tm1Pngr Pten^tm2Mak/Pten^tm2Mak Tg(Ckmm-cre)5Khn/0	involves: 129P2/OlaHsd * FVB	MGI:3619937
cx5	Pik3cd^tm1Tnr/Pik3cd^tm1Tnr Pik3cg^tm1Pngr/Pik3cg^tm1Pngr	B6.129-Pik3cd^tm1Tnr Pik3cg^tm1Pngr	MGI:3796518
cx6	Pik3cd^tm1Tnr/Pik3cd^tm1Tnr Pik3cg^tm1Pngr/Pik3cg^tm1Pngr	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6	MGI:3796506
cx7	Pik3cd^tm1Jni/Pik3cd^tm1Jni Pik3cg^tm1Pngr/Pik3cg^tm1Pngr	involves: 129P2/OlaHsd * C57BL/6	MGI:3796421
cx8	Ace2^tm1Pngr/Y Pik3cg^tm1Pngr/Pik3cg^tm1Pngr	involves: 129P2/OlaHsd * C57BL/6	MGI:5912245

Genotype
MGI:3796517

hm1

• Allelic Composition: Pik3cg^tm1Pngr/Pik3cg^tm1Pngr
• Genetic Background: B6.129P2-Pik3cg^tm1Pngr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

impaired neutrophil chemotaxis ( J:134324 )

• neutrophil migration in a chemotaxis assay with fMLP stimulation is reduced compared to for wild-type cells

decreased susceptibility to induced arthritis ( J:134324 )

• mice exhibit a 45% to 53% decrease in paw edema, reduced synovial inflammation, and reduced bone and cartilage erosion following administration of arthritogenic serum compared to wild-type mice

decreased inflammatory response ( J:134324 )

• mice exhibit a 45% to 53% decrease in paw edema, reduced synovial inflammation, and reduced bone and cartilage erosion following administration of arthritogenic serum compared to wild-type mice

skeleton

decreased susceptibility to induced arthritis ( J:134324 )

• mice exhibit a 45% to 53% decrease in paw edema, reduced synovial inflammation, and reduced bone and cartilage erosion following administration of arthritogenic serum compared to wild-type mice

cellular

impaired neutrophil chemotaxis ( J:134324 )

• neutrophil migration in a chemotaxis assay with fMLP stimulation is reduced compared to for wild-type cells

hematopoietic system

impaired neutrophil chemotaxis ( J:134324 )

• neutrophil migration in a chemotaxis assay with fMLP stimulation is reduced compared to for wild-type cells

Genotype
MGI:3619931

hm2

• Allelic Composition: Pik3cg^tm1Pngr/Pik3cg^tm1Pngr
• Genetic Background: involves: 129P2/OlaHsd

cardiovascular system

increased cardiac muscle contractility ( J:79151 )

• exhibit increased fractional shortening, velocity of circumferential fiber shortening, and peak aortic outflow velocity, indicating enhanced contractility

• individual cardiomyocytes display an increase in contractility

• treatment of cardiomyocytes with a cAMP blocker leads to a greater reduction in contractility compared to wild-type

abnormal cardiac muscle relaxation ( J:79151 )

• exhibit a significant increase in the rate of relaxation in cardiomyocytes despite the increase in peak contraction

muscle

increased cardiac muscle contractility ( J:79151 )

• exhibit increased fractional shortening, velocity of circumferential fiber shortening, and peak aortic outflow velocity, indicating enhanced contractility

• individual cardiomyocytes display an increase in contractility

• treatment of cardiomyocytes with a cAMP blocker leads to a greater reduction in contractility compared to wild-type

abnormal cardiac muscle relaxation ( J:79151 )

• exhibit a significant increase in the rate of relaxation in cardiomyocytes despite the increase in peak contraction

Genotype
MGI:3619226

hm3

• Allelic Composition: Pik3cg^tm1Pngr/Pik3cg^tm1Pngr
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

thymus hypoplasia ( J:60347 )

• reduction in thymic cellularity

decreased thymocyte number ( J:60347 )

abnormal NK cell differentiation ( J:124330 )

• mice have a higher percentage of CD117+ CD27+ NK cells and lower percentages of 2B4+ NK cells than in splenic NK cells from wild-type mice

abnormal T cell differentiation ( J:60347 )

• T cell development is altered however B cell development is normal

increased eosinophil cell number ( J:60347 )

increased neutrophil cell number ( J:60347 )

decreased CD4-positive, alpha-beta T cell number ( J:60347 )

• decrease in CD4+CD8- T cells in the spleen

increased monocyte cell number ( J:60347 )

abnormal immune cell physiology ( J:60347 )

• thymocytes exhibit an increase in apoptosis compared to wild-type after stimulation with adenosine receptor agonists

abnormal neutrophil physiology ( J:60347 )

• GRCR-induced respiratory burst is decreased in freshly isolated bone marrow neutrophils

impaired neutrophil chemotaxis ( J:60347 )

• 70% decrease in fMLP- and C5a-induced chemotaxis of neutrophils

impaired neutrophil recruitment ( J:60347 )

• accumulation of neutrophils in the peritoneal cavities is reduced in response to casein-induced peritonitis of Listeria infection

abnormal T cell physiology ( J:60347 )

• lymphocytic choriomeningitis virus (LCMV) induced footpad-swelling is reduced, indicating impaired CD+ T cell-dependent antiviral responses

abnormal T-helper 2 physiology ( J:60347 )

• production of antibodies to NIP after NIP-OVA immunization is reduced indicating impairment of functional T helper cell-dependent responses to hapten antigens

decreased cytotoxic T cell cytolysis ( J:124330 )

• NK cells are slightly less lytic than wild-type cells

abnormal T cell activation ( J:60347 )

decreased T cell proliferation ( J:60347 )

• T cells exhibit impaired proliferation in response to anti-CD3 epsilon on Con A stimulation, however exhibit normal proliferation in response to PMA/Ca+ inonaphore

decreased interferon-gamma secretion ( J:60347 , J:124330 )

• T cells produce lower amounts of interferon-gamma in response to treatment with anti-CD3 epsilon and anti-CD28, Con A, or PMA/Ca+ ionaphore (J:60347)

• mice exhibit decreased IFN-gamma secretion following NK1.1 or IL-12/IL-18 stimulation compared to in wild-type mice (J:124330)

decreased interleukin-2 secretion ( J:60347 )

• T cells produce lower amounts of IL-2 in response to treatment with anti-CD3 epsilon and anti-CD28, Con A, or PMA/Ca+ ionaphore

hematopoietic system

thymus hypoplasia ( J:60347 )

• reduction in thymic cellularity

decreased thymocyte number ( J:60347 )

abnormal definitive hematopoiesis ( J:60347 )

• increase in total and relative numbers of Gr1+Mac1+ myeloid cells in the spleen but not in bone marrow

abnormal NK cell differentiation ( J:124330 )

• mice have a higher percentage of CD117+ CD27+ NK cells and lower percentages of 2B4+ NK cells than in splenic NK cells from wild-type mice

abnormal T cell differentiation ( J:60347 )

• T cell development is altered however B cell development is normal

increased eosinophil cell number ( J:60347 )

increased neutrophil cell number ( J:60347 )

decreased CD4-positive, alpha-beta T cell number ( J:60347 )

• decrease in CD4+CD8- T cells in the spleen

increased monocyte cell number ( J:60347 )

abnormal neutrophil physiology ( J:60347 )

• GRCR-induced respiratory burst is decreased in freshly isolated bone marrow neutrophils

impaired neutrophil chemotaxis ( J:60347 )

• 70% decrease in fMLP- and C5a-induced chemotaxis of neutrophils

impaired neutrophil recruitment ( J:60347 )

• accumulation of neutrophils in the peritoneal cavities is reduced in response to casein-induced peritonitis of Listeria infection

abnormal T cell physiology ( J:60347 )

• lymphocytic choriomeningitis virus (LCMV) induced footpad-swelling is reduced, indicating impaired CD+ T cell-dependent antiviral responses

abnormal T-helper 2 physiology ( J:60347 )

• production of antibodies to NIP after NIP-OVA immunization is reduced indicating impairment of functional T helper cell-dependent responses to hapten antigens

decreased cytotoxic T cell cytolysis ( J:124330 )

• NK cells are slightly less lytic than wild-type cells

abnormal T cell activation ( J:60347 )

decreased T cell proliferation ( J:60347 )

• T cells exhibit impaired proliferation in response to anti-CD3 epsilon on Con A stimulation, however exhibit normal proliferation in response to PMA/Ca+ inonaphore

cellular

impaired neutrophil chemotaxis ( J:60347 )

• 70% decrease in fMLP- and C5a-induced chemotaxis of neutrophils

decreased T cell proliferation ( J:60347 )

• T cells exhibit impaired proliferation in response to anti-CD3 epsilon on Con A stimulation, however exhibit normal proliferation in response to PMA/Ca+ inonaphore

endocrine/exocrine glands

thymus hypoplasia ( J:60347 )

• reduction in thymic cellularity

decreased thymocyte number ( J:60347 )

Genotype
MGI:3619937

cn4

• Allelic Composition: Pik3cg^tm1Pngr/Pik3cg^tm1Pngr; Pten^tm2Mak/Pten^tm2Mak; Tg(Ckmm-cre)5Khn/0
• Genetic Background: involves: 129P2/OlaHsd * FVB

cardiovascular system

enlarged heart ( J:79151 )

• significantly enlarged compared to wild-type and single homozygous Pik3cg^tm1Pngr mice

cardiac hypertrophy ( J:79151 )

• extent of cardiac hypertrophy is similar to that seen in mice with cardiomyocyte-specific inactivation of Pten

increased cardiac muscle contractility ( J:79151 )

• hearts are hypercontractile as assessed by increased fractional shortening, velocity or circumferential fiber shortening, and peak aortic outflow velocity

• individual cardiomyocytes display increased contractility despite the hypertrophy

muscle

increased cardiac muscle contractility ( J:79151 )

• hearts are hypercontractile as assessed by increased fractional shortening, velocity or circumferential fiber shortening, and peak aortic outflow velocity

• individual cardiomyocytes display increased contractility despite the hypertrophy

growth/size/body

enlarged heart ( J:79151 )

• significantly enlarged compared to wild-type and single homozygous Pik3cg^tm1Pngr mice

cardiac hypertrophy ( J:79151 )

• extent of cardiac hypertrophy is similar to that seen in mice with cardiomyocyte-specific inactivation of Pten

Genotype
MGI:3796518

cx5

• Allelic Composition: Pik3cd^tm1Tnr/Pik3cd^tm1Tnr; Pik3cg^tm1Pngr/Pik3cg^tm1Pngr
• Genetic Background: B6.129-Pik3cd^tm1Tnr Pik3cg^tm1Pngr

immune system

impaired neutrophil chemotaxis ( J:134324 )

• neutrophil migration in a chemotaxis assay with LB4 or fMLP stimulation is reduced compared to for wild-type cells

• neutrophils travel shorter distances and at slower velocities than wild-type cells

impaired neutrophil recruitment ( J:134324 )

• neutrophils accumulation following LTB4-stimulation is reduced greater than 70% compared to in wild-type mice

decreased susceptibility to induced arthritis ( J:134324 )

• following administration of arthritogenic serum, mice develop minimal swelling unlike in wild-type mice

• 14 days following administration of autoreactive antibodies, mice exhibit relatively normal articular surfaces, intact joint spaces and the absence of periaticular inflammation unlike wild-type mice

decreased inflammatory response ( J:134324 )

• following administration of arthritogenic serum, mice develop minimal swelling unlike in wild-type mice

• 14 days following administration of autoreactive antibodies, mice exhibit relatively normal articular surfaces, intact joint spaces and the absence of periaticular inflammation unlike wild-type mice

skeleton

decreased susceptibility to induced arthritis ( J:134324 )

• following administration of arthritogenic serum, mice develop minimal swelling unlike in wild-type mice

• 14 days following administration of autoreactive antibodies, mice exhibit relatively normal articular surfaces, intact joint spaces and the absence of periaticular inflammation unlike wild-type mice

cellular

impaired neutrophil chemotaxis ( J:134324 )

• neutrophil migration in a chemotaxis assay with LB4 or fMLP stimulation is reduced compared to for wild-type cells

• neutrophils travel shorter distances and at slower velocities than wild-type cells

hematopoietic system

impaired neutrophil chemotaxis ( J:134324 )

• neutrophil migration in a chemotaxis assay with LB4 or fMLP stimulation is reduced compared to for wild-type cells

• neutrophils travel shorter distances and at slower velocities than wild-type cells

impaired neutrophil recruitment ( J:134324 )

• neutrophils accumulation following LTB4-stimulation is reduced greater than 70% compared to in wild-type mice

Genotype
MGI:3796506

cx6

• Allelic Composition: Pik3cd^tm1Tnr/Pik3cd^tm1Tnr; Pik3cg^tm1Pngr/Pik3cg^tm1Pngr
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6

immune system

abnormal thymus lobule morphology ( J:125791 )

• corticomedullary differentiation is lost

small thymus ( J:125791 )

thymus hypoplasia ( J:125791 )

• total thymus cell count is reduced 27-fold compared to in wild-type mice and 10-fold compared to in Pik3cg null mice

abnormal T cell differentiation ( J:125791 )

• the population of DN3 T cells is phenotypically different from in wild-type mice due to altered marker expression

decreased lymphocyte cell number ( J:125791 )

decreased T cell number ( J:125791 )

• peripheral lymph nodes and spleen exhibit decreased T cell populations compared to in wild-type mice

decreased double-positive T cell number ( J:125791 )

• mice have fewer double positive T cells due to increased apoptosis compared to in wild-type mice

decreased CD4-positive, alpha-beta T cell number ( J:125791 )

decreased CD8-positive, alpha-beta T cell number ( J:125791 )

abnormal T cell physiology ( J:125791 )

• calcium fluxes in response to TCR cross-linking are absent from T cells unlike in wild-type cells

hematopoietic system

abnormal thymus lobule morphology ( J:125791 )

• corticomedullary differentiation is lost

small thymus ( J:125791 )

thymus hypoplasia ( J:125791 )

• total thymus cell count is reduced 27-fold compared to in wild-type mice and 10-fold compared to in Pik3cg null mice

abnormal T cell differentiation ( J:125791 )

• the population of DN3 T cells is phenotypically different from in wild-type mice due to altered marker expression

decreased lymphocyte cell number ( J:125791 )

decreased T cell number ( J:125791 )

• peripheral lymph nodes and spleen exhibit decreased T cell populations compared to in wild-type mice

decreased double-positive T cell number ( J:125791 )

• mice have fewer double positive T cells due to increased apoptosis compared to in wild-type mice

decreased CD4-positive, alpha-beta T cell number ( J:125791 )

decreased CD8-positive, alpha-beta T cell number ( J:125791 )

abnormal T cell physiology ( J:125791 )

• calcium fluxes in response to TCR cross-linking are absent from T cells unlike in wild-type cells

endocrine/exocrine glands

abnormal thymus lobule morphology ( J:125791 )

• corticomedullary differentiation is lost

small thymus ( J:125791 )

thymus hypoplasia ( J:125791 )

• total thymus cell count is reduced 27-fold compared to in wild-type mice and 10-fold compared to in Pik3cg null mice

Genotype
MGI:3796421

cx7

• Allelic Composition: Pik3cd^tm1Jni/Pik3cd^tm1Jni; Pik3cg^tm1Pngr/Pik3cg^tm1Pngr
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal NK cell differentiation ( J:124330 )

• mice exhibit a high percentage of CD117+ and CD27+ NK cells and about 40% to 50% immature CD27 CD11b- cells compared to in wild-type mice

• NK cell development is blocked before the stage at which they acquire Ly49 receptors

decreased NK cell number ( J:124330 )

• mice exhibit a 60% to 70% reduction in NK cells in the spleen compared to in wild-type mice

decreased T cell number ( J:124330 )

• CD3+ T cell numbers and percentages are reduced compared to in wild-type mice

spleen hypoplasia ( J:124330 )

• total splenocyte numbers are reduced 50% compared to in wild-type mice

decreased cytotoxic T cell cytolysis ( J:124330 )

• NK cells are less lytic than wild-type cells

decreased interferon-gamma secretion ( J:124330 )

• mice exhibit decreased IFN-gamma secretion following NK1.1 or IL-12/IL-18 stimulation compared to in wild-type mice

hematopoietic system

abnormal NK cell differentiation ( J:124330 )

• mice exhibit a high percentage of CD117+ and CD27+ NK cells and about 40% to 50% immature CD27 CD11b- cells compared to in wild-type mice

• NK cell development is blocked before the stage at which they acquire Ly49 receptors

decreased NK cell number ( J:124330 )

• mice exhibit a 60% to 70% reduction in NK cells in the spleen compared to in wild-type mice

decreased T cell number ( J:124330 )

• CD3+ T cell numbers and percentages are reduced compared to in wild-type mice

spleen hypoplasia ( J:124330 )

• total splenocyte numbers are reduced 50% compared to in wild-type mice

decreased cytotoxic T cell cytolysis ( J:124330 )

• NK cells are less lytic than wild-type cells

Genotype
MGI:5912245

cx8

• Allelic Composition: Ace2^tm1Pngr/Y; Pik3cg^tm1Pngr/Pik3cg^tm1Pngr
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

cardiovascular system

increased cardiac muscle contractility ( J:124548 )

♂ • increase in basal myocardial contractility

♂ • mice exhibit a partial reversal of myocardial hypertrophy, prevention of neutrophil infiltration into myocardial tissue, and prevention of systolic function deterioration

muscle

increased cardiac muscle contractility ( J:124548 )

♂ • increase in basal myocardial contractility

♂ • mice exhibit a partial reversal of myocardial hypertrophy, prevention of neutrophil infiltration into myocardial tissue, and prevention of systolic function deterioration