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Gene Expression Data
Assay Details
Assay
Reference: J:182740 Moya IM, et al., Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades. Dev Cell. 2012 Mar 13;22(3):501-14
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8187623
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A Ctrl: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - Nrp1 (assay MGI:8187624).
Structure Level Pattern Image Note
TS15: future hindbrain Present 2A Ctrl right , 2A Ctrl left Sprouts emerged bilaterally from the perineural vascular plexi at the level of the otic vesicles and anastomosed medially in the roof of the hindbrain in a rostro-caudal fashion.
TS15: head blood vessel Present 2A Ctrl right , 2A Ctrl left Sprouts emerged bilaterally from the perineural vascular plexi at the level of the otic vesicles and anastomosed medially in the roof of the hindbrain in a rostro-caudal fashion.
Specimen 2A dKO: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Nrp1 (assay MGI:8187624).
Structure Level Pattern Image Note
TS15: future hindbrain Present 2A dKO left , 2A dKO right Large sinusoid-like vessels formed instead of the normal ramified network of capillaries. More pyramid-shaped and broader sprouts formed on both sides of mutant embryos, yet few or none anastomosed medially.
TS15: head blood vessel Present 2A dKO left , 2A dKO right Large sinusoid-like vessels formed instead of the normal ramified network of capillaries. More pyramid-shaped and broader sprouts formed on both sides of mutant embryos, yet few or none anastomosed medially.
Specimen 2D Ctrl 18s: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Structure Level Pattern Image Note
TS15: dorsal aorta Present 2D upper Ctrl
TS15: intersomitic blood vessel Present 2D upper Ctrl
Specimen 2D dKO 18s: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: dorsal aorta Present 2D upper dKO Increased sprouting in mutants, resulting in numerous ectopic intrasomitic vessels, in addition to the intersomitic vessels.
TS15: intersomitic blood vessel Present 2D upper dKO Increased sprouting in mutants, resulting in numerous ectopic intrasomitic vessels, in addition to the intersomitic vessels.
Specimen 2D Ctrl 27s: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Structure Level Pattern Image Note
TS15: dorsal aorta Present 2D lower Ctrl
TS15: intersomitic blood vessel Present 2D lower Ctrl
Specimen 2D dKO 27s: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: dorsal aorta Present 2D lower dKO Increased sprouting in mutants, resulting in numerous ectopic intrasomitic vessels, in addition to the intersomitic vessels.
TS15: intersomitic blood vessel Present 2D lower dKO Increased sprouting in mutants, resulting in numerous ectopic intrasomitic vessels, in addition to the intersomitic vessels.
Specimen 2F Ctrl: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - pH3.
Structure Cell Type Level Pattern Image Note
TS15: vascular plexus blood vessel endothelial cell Present 2F Ctrl
Specimen 2F dKO: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - pH3.
Structure Cell Type Level Pattern Image Note
TS15: vascular plexus blood vessel endothelial cell Present 2F dKO Significant reduction in the number of phospho-H3/Pecam1-positive proliferating cells in the dorsal vascular plexus.
Specimen 2G Ctrl: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - Golga2 (assay MGI:8187625).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel endothelial tip cell Present 2G Ctrl Tip cells were polarized with a compacted Golgi apparatus facing the leading edge of the cell.
Specimen 2G dKO: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Golga2 (assay MGI:8187625).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present 2G dKO The localization and compaction of the Golgi apparatus in tip-like cells of mutant embryos was random.
Specimen 2H Ctrl: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - Nrp1 (assay MGI:8187624).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present 2H Ctrl
Specimen 2H dKO: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Nrp1 (assay MGI:8187624).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present 2H dKO
Specimen 3A Ctrl upper: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - Smad1 (assay MGI:8187627) and Smad5 (assay MGI:8187821) (assay MGI:8187627) and Smad9 (assay MGI:8187822).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present 3A upper Ctrl left Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium.
Specimen 3A Ctrl lower: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - Smad1 (assay MGI:8187627) and Smad5 (assay MGI:8187821) (assay MGI:8187627) and Smad9 (assay MGI:8187822).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present 3A lower Ctrl left Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium.
TS15: head blood vessel endothelial stalk cell Present 3A lower Ctrl left , 3A lower Ctrl left enlarged Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium, including tip and stalk cells.
TS15: head blood vessel endothelial tip cell Present 3A lower Ctrl left , 3A lower Ctrl left enlarged Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium, including tip and stalk cells.
Specimen 3A dKO: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Smad1 (assay MGI:8187627) and Smad5 (assay MGI:8187821) (assay MGI:8187627) and Smad9 (assay MGI:8187822).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present 3A dKO left Nuclear p-Smad1/5/9 staining was absent in mutant endothelium but was normal elsewhere.
Specimen S2D Ctrl left: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - Des (assay MGI:8187634).
Structure Cell Type Level Pattern Image Note
TS15: yolk sac blood vessel blood vessel endothelial cell Present S2D Ctrl YS merge , S2D Ctrl YS Cd31
Specimen S2D dKO left: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Des (assay MGI:8187634).
Structure Cell Type Level Pattern Image Note
TS15: yolk sac blood vessel blood vessel endothelial cell Present S2D dKO YS merge , S2D dKO YS Cd31
Specimen S2D Ctrl right: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - Des (assay MGI:8187634).
Structure Cell Type Level Pattern Image Note
TS15: yolk sac blood vessel blood vessel endothelial cell Present S2D Ctrl Aorta merge , S2D Ctrl Aorta CD31
Specimen S2D dKO right: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Des (assay MGI:8187634).
Structure Cell Type Level Pattern Image Note
TS15: yolk sac blood vessel blood vessel endothelial cell Present S2D dKO Aorta merge , S2D dKO Aorta CD31
Specimen S3A Ctrl left: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - Golga2 (assay MGI:8187625).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel endothelial tip cell Present S3A Ctrl left Tip cells were polarized with a compacted Golgi apparatus facing the leading edge of the cell.
Specimen S3A Ctrl right: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: green - Pecam1; red - Golga2 (assay MGI:8187625).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel endothelial tip cell Present S3A Ctrl right Tip cells were polarized with a compacted Golgi apparatus facing the leading edge of the cell.
Specimen S3A dKO left: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Golga2 (assay MGI:8187625).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present S3A dKO-1 The localization and compaction of the Golgi apparatus in tip-like cells of mutant embryos was random.
Specimen S3A dKO middle: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Golga2 (assay MGI:8187625).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present S3A dKO-3 The localization and compaction of the Golgi apparatus in tip-like cells of mutant embryos was random.
Specimen S3A dKO right: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Golga2 (assay MGI:8187625).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present S3A dKO-5 The localization and compaction of the Golgi apparatus in tip-like cells of mutant embryos was random.
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03/18/2025
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