Gene Expression Data
Assay Details
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Assay
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Reference:
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J:182740
Moya IM, et al., Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades. Dev Cell. 2012 Mar 13;22(3):501-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:8187821
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Gene symbol:
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Smad5
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Gene name:
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SMAD family member 5
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Antibody:
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anti-phospho-Smad1/5/9 (9511)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Assay notes:
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The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody used was not detailed; all family members have been annotated.
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Results
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Specimen
3A Ctrl upper:
embryonic day 9.5; Smad1tm2Rob/Smad1 +, Smad5tm1Huy/Smad5 +
(more  )
Note: Double labeled: red - Smad5 and Smad1 and Smad9; green - Pecam1 (assay MGI:8187623).
Specimen
3A Ctrl upper:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6 * CD-1 * SJL
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Mutant Allele(s):
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Smad1tm2Rob/Smad1+
Smad5tm1Huy/Smad5+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Smad5 and Smad1 and Smad9; green - Pecam1 (assay MGI:8187623).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: head blood vessel |
blood vessel endothelial cell |
Present |
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3A upper Ctrl left
,
3A upper Ctrl right
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Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium.
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Specimen
3A Ctrl lower:
embryonic day 9.5; Smad1tm2Rob/Smad1 +, Smad5tm1Huy/Smad5 +
(more )
Note: Double labeled: red - Smad5 and Smad1 and Smad9; green - Pecam1 (assay MGI:8187623).
Specimen
3A Ctrl lower:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6 * CD-1 * SJL
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Mutant Allele(s):
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Smad1tm2Rob/Smad1+
Smad5tm1Huy/Smad5+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Smad5 and Smad1 and Smad9; green - Pecam1 (assay MGI:8187623).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: head blood vessel |
blood vessel endothelial cell |
Present |
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3A lower Ctrl left
,
3A lower Ctrl right
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Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium.
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| TS15: head blood vessel |
endothelial stalk cell |
Present |
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3A lower Ctrl left enlarged
,
3A lower Ctrl right
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3A lower Ctrl left
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3A lower Ctrl right enlarged
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Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium, including tip and stalk cells.
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| TS15: head blood vessel |
endothelial tip cell |
Present |
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3A lower Ctrl left enlarged
,
3A lower Ctrl right
,
3A lower Ctrl left
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3A lower Ctrl right enlarged
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Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium, including tip and stalk cells.
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Specimen
3A dKO:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6 * CD-1 * SJL
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Mutant Allele(s):
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Smad1tm2Rob/Smad1tm2Rob
Smad5tm1Huy/Smad5tm1Huy
Tg(Tek-cre)1Ywa/0
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Double labeled: red - Smad5 and Smad1 and Smad9; green - Pecam1 (assay MGI:8187623).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: head blood vessel |
blood vessel endothelial cell |
Absent |
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3A dKO left
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3A dKO right
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Nuclear p-Smad1/5/9 staining was absent in mutant endothelium but was normal elsewhere.
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Specimen
S4A:
embryonic day 9.5
(more )
Specimen
S4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Analysis Tools