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Gene Expression Data
Assay Details
Assay
Reference: J:182740 Moya IM, et al., Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades. Dev Cell. 2012 Mar 13;22(3):501-14
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8187822
Gene symbol: Smad9
Gene name: SMAD family member 9
Antibody: anti-phospho-Smad1/5/9 (9511)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Assay notes: The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody used was not detailed; all family members have been annotated.
Results
Specimen 3A Ctrl upper: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: red - Smad9 and Smad1 and Smad5; green - Pecam1 (assay MGI:8187623).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present 3A upper Ctrl left , 3A upper Ctrl right Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium.
Specimen 3A Ctrl lower: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: red - Smad9 and Smad1 and Smad5; green - Pecam1 (assay MGI:8187623).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present 3A lower Ctrl right , 3A lower Ctrl left Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium.
TS15: head blood vessel endothelial stalk cell Present 3A lower Ctrl left enlarged , 3A lower Ctrl right enlarged , 3A lower Ctrl left , 3A lower Ctrl right Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium, including tip and stalk cells.
TS15: head blood vessel endothelial tip cell Present 3A lower Ctrl left enlarged , 3A lower Ctrl right enlarged , 3A lower Ctrl left , 3A lower Ctrl right Ubiquitous nuclear p-Smad1/5/9 localization throughout the venous and arterial endothelium, including tip and stalk cells.
Specimen 3A dKO: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: red - Smad9 and Smad1 and Smad5; green - Pecam1 (assay MGI:8187623).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Absent 3A dKO right , 3A dKO left Nuclear p-Smad1/5/9 staining was absent in mutant endothelium but was normal elsewhere.
Specimen S4A: embryonic day 9.5 (more )
Structure Cell Type Level Pattern Image Note
TS15: dorsal aorta aortic endothelial cell Present S4A
TS15: cardinal vein vein endothelial cell Present S4A
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
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