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Gene Expression Data
Assay Details
Assay
Reference: J:182740 Moya IM, et al., Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades. Dev Cell. 2012 Mar 13;22(3):501-14
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8187624
Gene symbol: Nrp1
Gene name: neuropilin 1
Antibody: anti-Mouse/Rat Neuropilin1 Antibody (AF566)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 2A Ctrl: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: red - Nrp1; green - Pecam1 (assay MGI:8187623).
Structure Level Pattern Image Note
TS15: head blood vessel Present Regionally restricted 2A Ctrl right Expressed in the filopodia.
Specimen 2A dKO: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: red - Nrp1; green - Pecam1 (assay MGI:8187623).
Structure Level Pattern Image Note
TS15: head blood vessel Present Regionally restricted 2A dKO right Expressed in the filopodia. Large sinusoid-like vessels formed instead of the normal ramified network of capillaries. More pyramid-shaped and broader sprouts formed on both sides of mutant embryos, yet few or none anastomosed medially.
Specimen 2H Ctrl: embryonic day 9.5; Smad1tm2Rob/Smad1+, Smad5tm1Huy/Smad5+ (more )
Note: Double labeled: red - Nrp1; green - Pecam1 (assay MGI:8187623).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel endothelial tip cell Present Regionally restricted 2H Ctrl Expressed in the filopodia. Robust filopodia were restricted exclusively to tip cells.
Specimen 2H dKO: embryonic day 9.5; Smad1tm2Rob/Smad1tm2Rob, Smad5tm1Huy/Smad5tm1Huy, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: red - Nrp1; green - Pecam1 (assay MGI:8187623).
Structure Cell Type Level Pattern Image Note
TS15: head blood vessel blood vessel endothelial cell Present 2H dKO Expressed in the filopodia. All endothelial cells facing the migration front had numerous ectopic and spiky filopodia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory