Raf1
Gene Detail

Symbol Name ID

Raf1 v-raf-leukemia viral oncogene 1 MGI:97847

Synonyms

6430402F14Rik, c-Raf, Craf1, Raf-1, sarcoma 3611 oncogene, v-Raf

Feature Type

protein coding gene

Genetic Map

Chromosome 6

53.62 cM, cytoband C3
Detailed Genetic Map ± 1 cM


Mapping data(54)

Sequence Map

Chr6:115618067-115676635 bp, - strand From VEGA annotation of GRCm38   58569 bp   ±  kb flank VEGA Genome Browser | Ensembl Genome Browser | UCSC Browser | NCBI Map Viewer

Mouse Genome Browser

Vertebrate homology

HomoloGene:48145  Vertebrate Homology Class
1 human; 1 mouse; 1 rat; 1 chimpanzee; 1 rhesus macaque; 1 dog; 1 chicken; 1 zebrafish

Protein SuperFamily: RAF proto-oncogene serine/threonine-protein kinase
Gene Tree: Raf1

Human homologs

Human Homolog		RAF1, v-raf-1 murine leukemia viral oncogene homolog 1
NCBI Gene ID		5894
neXtProt AC		NX_P04049
Human Synonyms		c-Raf, CRAF, NS5, Raf-1
Human Chr (Location)		3p25; chr3:12625100-12705700 (-)  GRCh37.p10
Disease Associations		(2) Diseases Associated with Human RAF1

Alleles and phenotypes

All alleles(155) : Targeted(11) Gene trapped(143) Transgenic(1)
 

Homozygotes for targeted null mutations are growth retarded, with hypocellular fetal livers, placental anomalies, and defects of skin and lungs, resulting in lethality around mid-gestation. Mice heterozygous for a knock-in allele exhibit hypertrophic cardiomyopathy.

 
Human Diseases Modeled Using Mouse Raf1 (1)    Alleles Annotated to Human Diseases(2)   

Gene Ontology (GO) classifications

All GO classifications: (41 annotations)

Process	activation of MAPKK activity, apoptotic process, ...
Component	cytoplasm, cytosol, ...
Function	ATP binding, identical protein binding, ...

External Resources: FuncBase

Expression

Literature Summary: (12 records)
Data Summary: Results (126)    Tissues (72)    Images (16)
Theiler Stages: 2, 3, 4, 5, 11, 15, 17, 19, 20, 21, 22, 23, 24, 25, 28

Assay Type	Results
Immunohistochemistry	8
RNA in situ	25
Northern blot	63
RT-PCR	30

cDNA source data(15)
External Resources: Allen Institute   GEO   ArrayExpress

Molecular reagents

All nucleic(34) Genomic(4) cDNA(20) Primer pair(3) Other(7)
Microarray probesets(5)

Other database links

VEGA Gene Model	OTTMUSG00000020697 (Evidence)
Ensembl Gene Model	ENSMUSG00000000441 (Evidence)
Entrez Gene	110157 (Evidence)
DFCI	TC1571969, TC1603648, TC1657728, TC1733209
EC	2.7.11.1
Consensus CDS Project	CCDS20441.1
International Mouse Knockout Project Status	Raf1

Sequences

Length	Strain/Species
genomic	OTTMUSG00000020697	VEGA Gene Model | MGI Sequence Detail	58569	C57BL/6J
transcript	OTTMUST00000048983	VEGA | MGI Sequence Detail	3070	Not Applicable
polypeptide	OTTMUSP00000022608	VEGA | MGI Sequence Detail	648	Not Applicable

All sequences(55) RefSeq(2) UniProt(5)

Polymorphisms

RFLP(10) : SNPs(184 from dbSNP Build 128)

Protein-related information

Resource	ID	Description
InterPro	IPR020454	Diacylglycerol/phorbol-ester binding
InterPro	IPR017441	Protein kinase, ATP binding site
InterPro	IPR000719	Protein kinase, catalytic domain
InterPro	IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol binding
InterPro	IPR011009	Protein kinase-like domain
InterPro	IPR003116	Raf-like Ras-binding
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR001245	Serine-threonine/tyrosine-protein kinase catalytic domain
Protein Ontology	PR:000003244	RAF proto-oncogene serine/threonine-protein kinase

References

(Earliest) J:7145 Rapp UR, et al., Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4218-22
(Latest) J:189143 Wu X, et al., Increased BRAF heterodimerization is the common pathogenic mechanism for noonan syndrome-associated RAF1 mutants. Mol Cell Biol. 2012 Oct;32(19):3872-90
All references(172)

Other accession IDs

MGD-MRK-13751, MGD-MRK-13752, MGI:1277195, MGI:1924126, MGI:2141729, MGI:2146790