Nos3
Gene Detail

Symbol Name ID

Nos3 nitric oxide synthase 3, endothelial cell MGI:97362

Synonyms

2310065A03Rik, ecNOS, eNOS, Nos-3

Feature Type

protein coding gene

Genetic Map

Chromosome 5

11.32 cM
Detailed Genetic Map ± 1 cM


Mapping data(8)

Sequence Map

Chr5:24364810-24384474 bp, + strand From VEGA annotation of GRCm38   19665 bp   ±  kb flank VEGA Genome Browser | Ensembl Genome Browser | UCSC Browser | NCBI Map Viewer

Mouse Genome Browser

Vertebrate homology

HomoloGene:504  Vertebrate Homology Class
1 human; 1 mouse; 1 rat; 1 chimpanzee; 1 dog

Protein SuperFamily: nitric-oxide synthase, inducible
Gene Tree: Nos3

Human homologs

Human Homolog		NOS3, nitric oxide synthase 3 (endothelial cell)
NCBI Gene ID		4846
neXtProt AC		NX_P29474
Human Synonyms		ECNOS, eNOS
Human Chr (Location)		7q36; chr7:150688144-150711687 (+)  GRCh37.p10
Disease Associations		(5) Diseases Associated with Human NOS3

Alleles and phenotypes

All alleles(7) : Targeted(6) Gene trapped(1)
 

Homozygotes for targeted null mutations exhibit reduced survival, hypertension, inhibited basal vasodilation, insulin resistance, fewer mitochondria, reduced heart rate, impaired ovulation and, in some, shortened limbs.

 
Human Diseases Modeled Using Mouse Nos3 (2)    Alleles Annotated to Human Diseases(3)    Phenotype Images(1)

Gene Ontology (GO) classifications

All GO classifications: (67 annotations)

Process	angiogenesis, arginine catabolic process, ...
Component	apical part of cell, caveola, ...
Function	actin monomer binding, arginine binding, ...

External Resources: FuncBase

Expression

Literature Summary: (38 records)
Data Summary: Results (252)    Tissues (150)    Images (9)
Theiler Stages: 20, 22, 23, 24, 25, 26, 28

Assay Type	Results
RNA in situ	195
RT-PCR	57

cDNA source data(11)
External Resources: Allen Institute   GEO   ArrayExpress

Molecular reagents

All nucleic(18) cDNA(12) Primer pair(6)
Microarray probesets(5)

Other database links

VEGA Gene Model	OTTMUSG00000024082 (Evidence)
Ensembl Gene Model	ENSMUSG00000028978 (Evidence)
Entrez Gene	18127 (Evidence)
NIA Mouse Gene Index	U005359
EC	1.14.13.39
Consensus CDS Project	CCDS19117.1
International Mouse Knockout Project Status	Nos3

Sequences

Length	Strain/Species
genomic	OTTMUSG00000024082	VEGA Gene Model | MGI Sequence Detail	19665	C57BL/6J
transcript	OTTMUST00000058885	VEGA | MGI Sequence Detail	4132	Not Applicable
polypeptide	OTTMUSP00000028591	VEGA | MGI Sequence Detail	1202	Not Applicable

All sequences(25) RefSeq(2) UniProt(3)

Polymorphisms

RFLP(1) : SNPs(106 from dbSNP Build 128)

Protein-related information

Resource	ID	Description
InterPro	IPR003097	FAD-binding, type 1
InterPro	IPR017927	Ferredoxin reductase-type FAD-binding domain
InterPro	IPR001094	Flavodoxin
InterPro	IPR008254	Flavodoxin/nitric oxide synthase
InterPro	IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase
InterPro	IPR023173	NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain-3
InterPro	IPR026009	Nitric-oxide synthase
InterPro	IPR012144	Nitric-oxide synthase, metazoa
InterPro	IPR004030	Nitric oxide synthase, oxygenase domain
InterPro	IPR001433	Oxidoreductase FAD/NAD(P)-binding
InterPro	IPR017938	Riboflavin synthase-like beta-barrel
Protein Ontology	PR:000011328	nitric oxide synthase, endothelial

References

(Earliest) J:19282 O'Dell TJ, et al., Endothelial NOS and the blockade of LTP by NOS inhibitors in mice lacking neuronal NOS. Science. 1994 Jul 22;265(5171):542-6
(Latest) J:194537 Stobart JL, et al., Astrocyte-induced cortical vasodilation is mediated by D-serine and endothelial nitric oxide synthase. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3149-54
All references(533)

Other accession IDs

MGD-MRK-12911, MGD-MRK-12914, MGI:1919183