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Nos3 Gene Detail
Summary
  • Symbol
    Nos3
  • Name
    nitric oxide synthase 3, endothelial cell
  • Synonyms
    2310065A03Rik, ecNOS, eNOS, Nos-3
  • Feature Type
    protein coding gene
  • IDs
    MGI:97362
    NCBI Gene: 18127
  • Alliance
    genome feature page
  • Transcription Start Sites
    3 TSS
Location &
Maps
more
  • Sequence Map
    Chr5:24569808-24589472 bp, + strand
    From Ensembl annotation of GRCm39
  • View this region in JBrowse
  • Genome Browsers
    Ensembl | UCSC | NCBI
  • Genetic Map
    Chromosome 5, 11.32 cM
  • Mapping Data
    8 experiments
Strain
Comparison
more
  • SNPs within 2kb
    534 from dbSNP Build 142
  • Strain Annotations
    19
  • RFLP
    1
For selected strains:
Strain Gene Model ID Feature Type Coordinates Select Strains
C57BL/6J MGI_C57BL6J_97362
 protein coding gene Chr5:24569772-24589472 (+)
129S1/SvImJ ENSMUSG00200012756
 protein coding gene Chr5:20176803-20196440 (+)
A/J ENSMUSG00195039762
 protein coding gene Chr5:19647768-19667431 (+)
AKR/J ENSMUSG00220032877
 protein coding gene Chr5:19730406-19750081 (+)
BALB/cJ ENSMUSG00180024465
 protein coding gene Chr5:19514740-19534416 (+)
C3H/HeJ ENSMUSG00175044169
 protein coding gene Chr5:21446009-21465667 (+)
C57BL/6NJ ENSMUSG00215039634
 protein coding gene Chr5:20081685-20101356 (+)
CAROLI/EiJ MGP_CAROLIEiJ_G0027006
 protein coding gene Chr5:19279784-19299654 (+)
CAST/EiJ ENSTCUG00005003102
 protein coding gene Chr5:19650502-19670514 (+)
CBA/J ENSMUSG00210042917
 protein coding gene Chr5:20485291-20504951 (+)
DBA/2J ENSMUSG00185035928
 protein coding gene Chr5:22017631-22037185 (+)
FVB/NJ ENSMUSG00205018143
 protein coding gene Chr5:19237767-19257445 (+)
JF1/MsJ ENSUMUG00000002549
 protein coding gene Chr5:26580514-26600083 (+)
LP/J ENSMUSG00230024666
 protein coding gene Chr5:26458801-26478430 (+)
NOD/ShiLtJ ENSMUSG00190004191
 protein coding gene Chr5:19551740-19571370 (+)
NZO/HlLtJ ENSMUSG00225015923
 protein coding gene Chr5:31867827-31887516 (+)
PWK/PhJ ENSLUMG00010031314
 protein coding gene Chr5:19619117-19638707 (+)
SPRET/EiJ ENSMSPG00010033283
 protein coding gene Chr5:20484845-20504786 (+)
WSB/EiJ ENSIUOG00005041354
 protein coding gene Chr5:22374179-22393854 (+)



Homology
more
  • Human Ortholog
    NOS3, nitric oxide synthase 3
  • Vertebrate Orthologs
    2
Vertebrate Orthology Source
Alliance of Genome Resources
  • Human Ortholog
    NOS3, nitric oxide synthase 3
  • Synonyms
    cNOS, EC-NOS, ECNOS, eNOS, MYMY8, NOSIII
  • Links
    NCBI Gene ID: 4846
    UniProt: P29474

  • Chr Location
    7q36.1; chr7:150991017-151015653 (+)  GRCh38
Human Diseases
more
  • Diseases
    3 with Nos3 mouse models; 38 with human NOS3 associations

Human Disease Mouse Models
      
IDs
 View 3 models
      
IDs
 View 1 model
IDs
 View 1 model
      
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Click on a disease name to see all genes associated with that disease.

  • Mutations/Alleles
    3 with disease annotations
  • References
    9 with disease annotations
Mutations,
Alleles, and
Phenotypes
less
  • Phenotype Summary
    233 phenotypes from 11 alleles in 19 genetic backgrounds
    88 phenotypes from multigenic genotypes
    1 images
    718 phenotype references
Phenotype Overview

adipose tissue
behavior/neurological
cardiovascular system
cellular
craniofacial
digestive/alimentary system
embryo
endocrine/exocrine glands
growth/size/body
hearing/vestibular/ear
hematopoietic system
homeostasis/metabolism
integument
immune system
limbs/digits/tail
liver/biliary system
mortality/aging
muscle
nervous system
pigmentation
renal/urinary system
reproductive system
respiratory system
skeleton
taste/olfaction
neoplasm
vision/eye

Click cells to view annotations.
Homozygotes for targeted null mutations exhibit reduced survival, hypertension, inhibited basal vasodilation, insulin resistance, fewer mitochondria, reduced heart rate, impaired ovulation and, in some, shortened limbs.
Gene Ontology
(GO)
Classifications
less
  • All GO Annotations
    138
  • GO References
    33
Molecular Function

carbohydrate derivative binding
cytoskeletal protein binding
DNA binding
enzyme regulator
hydrolase
ligase
lipid binding
oxidoreductase
RNA binding
signaling receptor activity
signaling receptor binding
transcription
transferase
transporter
Biological Process

carbohydrate derivative metabolism
cell differentiation
cell population proliferation
cellular component organization
DNA-templated transcription
establishment of localization
homeostatic process
immune system process
lipid metabolic process
programmed cell death
protein metabolic process
response to stimulus
signaling
system development
Cellular Component

cell projection
cytoplasmic vesicle
cytoskeleton
cytosol
endoplasmic reticulum
endosome
extracellular region
Golgi apparatus
mitochondrion
membraneless organelle
nucleus
organelle envelope
organelle lumen
plasma membrane
protein-containing complex
synapse
vacuole
Click cells to view annotations.
Expression
less
Expression Overview

early conceptus
embryo ectoderm
embryo endoderm
embryo mesoderm
embryo mesenchyme
extraembryonic component
alimentary system
auditory system
branchial arches
cardiovascular system
connective tissue
endocrine system
exocrine system
hemolymphoid system
integumental system
limbs
liver and biliary system
musculoskeletal system
nervous system
olfactory system
reproductive system
respiratory system
urinary system
visual system
Click cells to view annotations.


  • Assay Results
    1005
  • Tissues
    220
  • cDNA Data
    13
  • Literature Summary
    75
  • Comparison Matrix
    Gene Expression + Phenotype
    • Sequences &
    Gene Models
    less
    Representative SequencesLengthStrain/SpeciesFlank
    genomic ENSMUSG00000028978 Ensembl Gene Model | MGI Sequence Detail 19665 C57BL/6J ±  kb
    transcript ENSMUST00000030834 Ensembl | MGI Sequence Detail 4132 Not Applicable  
    polypeptide ENSMUSP00000030834 Ensembl | MGI Sequence Detail 1202 Not Applicable  
    For the selected sequence
    Protein
    Information
    less
    • UniProt
      3 Sequences
    • Protein Ontology
      PR:000011328 nitric oxide synthase 3
      (term hierarchy)
    • EC
      1.14.13.39
    • InterPro Domains
      IPR017927 FAD-binding domain, ferredoxin reductase-type
      IPR039261 Ferredoxin-NADP reductase (FNR), nucleotide-binding domain
      IPR001094 Flavodoxin-like
      IPR008254 Flavodoxin/nitric oxide synthase
      IPR029039 Flavoprotein-like superfamily
      IPR001709 Flavoprotein pyridine nucleotide cytochrome reductase
      IPR023173 NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily
      IPR044943 Nitric oxide synthase, domain 1 superfamily
      IPR044940 Nitric oxide synthase, domain 2 superfamily
      IPR044944 Nitric oxide synthase, domain 3 superfamily
      IPR012144 Nitric-oxide synthase, eukaryote
      IPR050607 Nitric Oxide Synthase (NOS)
      IPR004030 Nitric oxide synthase, N-terminal
      IPR036119 Nitric oxide synthase, N-terminal domain superfamily
      IPR001433 Oxidoreductase FAD/NAD(P)-binding
      IPR017938 Riboflavin synthase-like beta-barrel
      IPR003097 Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding
    • GlyGen
      P70313 2 sites, 1 O-linked glycan (1 site)
    Molecular
    Reagents
    less
    • All nucleic 26
      Genomic 1
      cDNA 14
      Primer pair 11
      Antibodies 11

      Microarray probesets 5
    Other
    Accession IDs
    less
    MGD-MRK-12911, MGD-MRK-12914, MGI:1919183
    References
    more
    • Summaries
      All 1024
      Developmental Gene Expression 75
      Diseases 9
      Gene Ontology 33
      Phenotypes 718
    • Earliest
      J:109968 Roderick TH, et al., Nineteen paracentric chromosomal inversions in mice. Genetics. 1974 Jan;76(1):109-17
    • Latest
      J:390609 Baldwin HS, et al., Endocardial TIE2 orchestrates cardiac trabeculation via sequestering FOXO1 within the cytoplasm and stimulating versican cleavage. Development. 2026 Jul 15;153(14):dev205674
    Contributing Projects:
    Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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    Funding Information
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    Send questions and comments to User Support.     		last database update
    09/01/2026
    MGI 6.24     		The Jackson Laboratory