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Nos3
Gene Detail
Symbol

Name
ID
Nos3
nitric oxide synthase 3, endothelial cell
MGI:97362
Synonyms
2310065A03Rik, ecNOS, eNOS, Nos-3
Feature Type
protein coding gene
Genetic Map
Chromosome 5
11.32 cM
Detailed Genetic Map ± 1 cM


Mapping data(8)
Sequence Map
Chr5:24364810-24384474 bp, + strand
From VEGA annotation of GRCm38

  19665 bp   ±  kb flank

VEGA Genome Browser | Ensembl Genome Browser | UCSC Browser | NCBI Map Viewer


Mouse Genome Browser
Vertebrate
homology
HomoloGene:504  Vertebrate Homology Class
1 human; 1 mouse; 1 rat; 1 chimpanzee; 1 rhesus macaque; 1 cattle; 1 dog; 1 western clawed frog

HCOP human homology predictions: NOS3
Protein SuperFamily: nitric-oxide synthase, inducible
Gene Tree: Nos3

Human
homologs
NOS3, nitric oxide synthase 3 (endothelial cell)
Orthology source: HomoloGene, HGNC

IDs:
NCBI Gene ID: 4846
neXtProt AC: NX_P29474

Human Synonyms: ECNOS, eNOS

Human Chr (Location): 7q36; chr7:150991056-151014599 (+)  GRCh38.p2

Disease Associations: (5) Diseases Associated with Human NOS3

Mutations,
alleles, and
phenotypes
All mutations/alleles(9) : Gene trapped(1) Targeted(8)
Incidental mutations (data from Mutagenetix , APF )
 
Homozygotes for targeted null mutations exhibit reduced survival, hypertension, inhibited basal vasodilation, insulin resistance, fewer mitochondria, reduced heart rate, impaired ovulation and, in some, shortened limbs.
 
Human Diseases Modeled in Mice Using Nos3 (2)    Mutations Annotated to Human Diseases (3)    Phenotype Images(1)
Interactions
Nos3 interacts with 68 markers (Mir7-2, Mir7b, Mir92-1, ...)
Gene Ontology
(GO)
classifications
All GO classifications: (75 annotations)
Process angiogenesis, arginine catabolic process, ...
Component apical part of cell, caveola, ...
Function actin binding, actin monomer binding, ...
External Resources: FuncBase
Expression
Literature Summary: (42 records)
Data Summary: Results (272)    Tissues (167)    Images (9)    Tissue x Stage Matrix (view)
Assay TypeResults
Immunohistochemistry 3
RNA in situ 209
RT-PCR 60
cDNA source data(11)
Other mouse links: Allen Institute   GEO   Expression Atlas
Other vertebrate links: Xenbase nos3    NEW 
Molecular
reagents
All nucleic(20) Genomic(1) cDNA(12) Primer pair(7)
Microarray probesets(5)
Other database
links
VEGA Gene Model OTTMUSG00000024082 (Evidence)
Ensembl Gene Model ENSMUSG00000028978 (Evidence)
Entrez Gene 18127 (Evidence)
NIA Mouse Gene Index U005359
EC 1.14.13.39
Consensus CDS Project CCDS19117.1
International Mouse Phenotyping Consortium Status Nos3
Sequences
Representative SequencesLengthStrain/SpeciesFlank
genomic OTTMUSG00000024082 VEGA Gene Model | MGI Sequence Detail 19665 C57BL/6J ±  kb
transcript OTTMUST00000058885 VEGA | MGI Sequence Detail 4132 Not Applicable 
polypeptide OTTMUSP00000028591 VEGA | MGI Sequence Detail 1202 Not Applicable 

For the selected sequences
All sequences(27) RefSeq(4) UniProt(3)
Polymorphisms
RFLP(1) : SNPs within 2kb(159 from dbSNP Build 137)
Protein-related
information
ResourceIDDescription
InterPro IPR003097 FAD-binding, type 1
InterPro IPR017927 Ferredoxin reductase-type FAD-binding domain
InterPro IPR001094 Flavodoxin
InterPro IPR008254 Flavodoxin/nitric oxide synthase
InterPro IPR001709 Flavoprotein pyridine nucleotide cytochrome reductase
InterPro IPR023173 NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain-3
InterPro IPR012144 Nitric-oxide synthase, metazoa
InterPro IPR004030 Nitric oxide synthase, oxygenase domain
InterPro IPR001433 Oxidoreductase FAD/NAD(P)-binding
InterPro IPR017938 Riboflavin synthase-like beta-barrel
Protein Ontology PR:000011328 nitric oxide synthase, endothelial
References
(Earliest) J:19282 O'Dell TJ, et al., Endothelial NOS and the blockade of LTP by NOS inhibitors in mice lacking neuronal NOS. Science. 1994 Jul 22;265(5171):542-6
(Latest) J:220740 Shinmura K, et al., Indispensable role of endothelial nitric oxide synthase in caloric restriction-induced cardioprotection against ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2015 Apr 15;308(8):H894-903
All references(601)
Disease annotation references (6)
Other
accession IDs
MGD-MRK-12911, MGD-MRK-12914, MGI:1919183

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
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last database update
06/23/2015
MGI 5.22
The Jackson Laboratory