Polr2b MGI Mouse Gene Detail - MGI:2388280 - polymerase (RNA) II (DNA directed) polypeptide B

Symbol

Polr2b
Name

polymerase (RNA) II (DNA directed) polypeptide B
Synonyms

MGC:47046, RPB2

Feature Type

protein coding gene
IDs

MGI:2388280
NCBI Gene: 231329
Alliance

genome feature page
Transcription Start Sites

3 TSS

Sequence Map

Chr5:77458331-77497175 bp, + strand
From NCBI annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 5, 41.66 cM
Mapping Data

1 experiment

SNPs within 2kb

955 from dbSNP Build 142
Strain Annotations

19

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_2388280	protein coding gene	Chr5:77457994-77497175 (+)
129S1/SvImJ	ENSMUSG00200000839	protein coding gene	Chr5:73148475-73187692 (+)
A/J	ENSMUSG00195010637	protein coding gene	Chr5:72454817-72494083 (+)
AKR/J	ENSMUSG00220014456	protein coding gene	Chr5:72568612-72607880 (+)
BALB/cJ	ENSMUSG00180000051	protein coding gene	Chr5:72222668-72261949 (+)
C3H/HeJ	ENSMUSG00175004090	protein coding gene	Chr5:74257939-74297208 (+)
C57BL/6NJ	ENSMUSG00215000420	protein coding gene	Chr5:73034843-73074120 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0027329	protein coding gene	Chr5:70316063-70355463 (+)
CAST/EiJ	ENSTCUG00005013320	protein coding gene	Chr5:72003481-72042911 (+)
CBA/J	ENSMUSG00210000454	protein coding gene	Chr5:73195834-73235105 (+)
DBA/2J	ENSMUSG00185016437	protein coding gene	Chr5:75025782-75065056 (+)
FVB/NJ	ENSMUSG00205001074	protein coding gene	Chr5:71984165-72023436 (+)
JF1/MsJ	ENSUMUG00000004567	protein coding gene	Chr5:79520926-79558433 (+)
LP/J	ENSMUSG00230020686	protein coding gene	Chr5:79722393-79761607 (+)
NOD/ShiLtJ	ENSMUSG00190007685	protein coding gene	Chr5:72147924-72187196 (+)
NZO/HlLtJ	ENSMUSG00225033057	protein coding gene	Chr5:84769504-84808716 (+)
PWK/PhJ	ENSLUMG00010007375	protein coding gene	Chr5:72086374-72125852 (+)
SPRET/EiJ	ENSMSPG00010009132	protein coding gene	Chr5:74116037-74155360 (+)
WSB/EiJ	ENSIUOG00005018225	protein coding gene	Chr5:74992251-75031627 (+)

Human Ortholog

POLR2B, RNA polymerase II subunit B

Vertebrate Orthologs

3

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

POLR2B, RNA polymerase II subunit B
Synonyms

hRPB140, POL2RB, RPB2
Links

HGNC:9188

NCBI Gene ID: 5431

UniProt: P30876
Chr Location

4q12; chr4:56977722-57031158 (+) GRCh38

MGI Vertebrate Homology

Polr2b stringent orthology
1 human;1 mouse;1 rat;1 zebrafish
HCOP

vertebrate homology predictions: POLR2B
Gene Tree

Polr2b

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Phenotype Summary

6 phenotypes from 1 allele in 2 genetic backgrounds
1 images
18 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

24
Chemically induced (other)

2
Endonuclease-mediated

3
Gene trapped

16
Spontaneous

1
Targeted

2
Genomic Mutations

3 involving Polr2b
Incidental Mutations

Mutagenetix , APF , CvDC
Find Mice (IMSR)

60 strains or lines available

Mice homozygous for a knock-out allele show abnormal morula morphology and complete embryonic lethality before implantation. In vitro, embryos fail to hatch from the zona pellucida and die after 3 days in culture.

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All GO Annotations

38
GO References

10

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

847
Tissues

120
cDNA Data

10
Literature Summary

4

Images

Tissue x Stage Matrix

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

Xenbase polr2b

ZFIN polr2b

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All Sequences

38
RefSeq

2
UniProt

1
CCDS

CCDS51528.1

Ensembl

ENSMUSG00000029250 (Evidence)
NCBI Gene

231329

			Length	Strain/Species	Flank
genomic	231329	NCBI Gene Model \| MGI Sequence Detail	38845	C57BL/6J	± kb
transcript	NM_153798	RefSeq \| MGI Sequence Detail	3812	C57BL/6
polypeptide	Q8CFI7	UniProt \| EBI \| MGI Sequence Detail	1174	Not Applicable

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UniProt

1 Sequence
Protein Ontology

PR:000012988 DNA-directed RNA polymerase II subunit RPB2

(term hierarchy)
EC

2.7.7.48, 2.7.7.6, 3.1.13.-
InterPro Domains

IPR015712 DNA-directed RNA polymerase, subunit 2

IPR007120 DNA-directed RNA polymerase, subunit 2, hybrid-binding domain

IPR037033 DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily

IPR007121 RNA polymerase, beta subunit, conserved site

IPR007644 RNA polymerase, beta subunit, protrusion

IPR007642 RNA polymerase Rpb2, domain 2

IPR037034 RNA polymerase Rpb2, domain 2 superfamily

IPR007645 RNA polymerase Rpb2, domain 3

IPR007646 RNA polymerase Rpb2, domain 4

IPR007647 RNA polymerase Rpb2, domain 5

IPR007641 RNA polymerase Rpb2, domain 7

IPR014724 RNA polymerase Rpb2, OB-fold
GlyGen

Q8CFI7 2 sites, 1 O-linked glycan (2 sites)

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All nucleic 11

cDNA 10

Primer pair 1

Microarray probesets 2

Summaries

All 56

Developmental Gene Expression 4

Gene Ontology 10

Phenotypes 18
Earliest

J:109968 Roderick TH, et al., Nineteen paracentric chromosomal inversions in mice. Genetics. 1974 Jan;76(1):109-17
Latest

J:379205 Chovatiya G, et al., Cell-type-specific RNA polymerase II activity maps in intact tissues provide a gateway to mammalian gene regulatory mechanisms in vivo. Dev Cell. 2026 Feb 11;61(2):434-451.e8

TSS for Polr2b:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr46788	Chr5:77458325-77458392 (+)	28 bp
Tssr46789	Chr5:77460390-77460407 (+)	2,068 bp
Tssr46790	Chr5:77497085-77497123 (+)	38,773 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 09/15/2026 MGI 6.29