Lig4
Gene Detail

Symbol Name ID

Lig4 ligase IV, DNA, ATP-dependent MGI:1335098

Synonyms

5830471N16Rik, DNA ligase IV, tiny

Feature Type

protein coding gene

Genetic Map

Chromosome 8

4.52 cM
Detailed Genetic Map ± 1 cM


Mapping data(1)

Sequence Map

Chr8:9970020-9976323 bp, - strand From NCBI annotation of GRCm38   6304 bp   ±  kb flank VEGA Genome Browser | Ensembl Genome Browser | UCSC Browser | NCBI Map Viewer

Mouse Genome Browser

Vertebrate homology

HomoloGene:1736  Vertebrate Homology Class
1 human; 1 mouse; 1 rat; 1 chimpanzee; 1 cattle; 1 dog; 1 chicken; 1 zebrafish

Gene Tree: Lig4

Human homologs

Human Homolog		LIG4, ligase IV, DNA, ATP-dependent
NCBI Gene ID		3981
neXtProt AC		NX_P49917
Human Chr (Location)		13q33-q34; chr13:108859790-108870716 (-)  GRCh37.p10
Disease Associations		(3) Diseases Associated with Human LIG4

Alleles and phenotypes

All alleles(11) : Targeted(6) Gene trapped(4) Chemically induced(1)
 

Null homozygotes die late in gestation with extensive CNS apoptosis, blocked lymphopoeiesis and failure of V(D)J joining. Carrier fibroblasts show elevated chromosome breaks. ~40% of homozygous hypomorphs survive, with retarded growth, reduced PBL and progressive loss of hematopoietic stem cells.

 
Human Diseases Modeled Using Mouse Lig4 (1)    Alleles Annotated to Human Diseases(3)   

Gene Ontology (GO) classifications

All GO classifications: (81 annotations)

Process	cell cycle, cell division, ...
Component	chromosome, condensed chromosome, ...
Function	ATP binding, DNA binding, ...

This is a GO Consortium Reference Genome Project gene.
External Resources: FuncBase

Expression

Literature Summary: (4 records)
Data Summary: Results (97)    Tissues (96)   
Theiler Stages: 22, 26, 28

Assay Type	Results
RNA in situ	94
RT-PCR	3

cDNA source data(33)
External Resources: Allen Institute   GEO   ArrayExpress

Molecular reagents

All nucleic(37) Genomic(1) cDNA(33) Primer pair(2) Other(1)
Microarray probesets(2)

Other database links

Ensembl Gene Model	ENSMUSG00000049717 (Evidence)
Entrez Gene	319583 (Evidence)
UniGene	80584
DFCI	TC1583831, TC1608504, TC1677445
DoTS	DT.101726060, DT.525997
NIA Mouse Gene Index	U029574
EC	6.5.1.1
Consensus CDS Project	CCDS22092.1
International Mouse Knockout Project Status	Lig4

Sequences

Length	Strain/Species
genomic	319583	NCBI Gene Model | MGI Sequence Detail	6304	C57BL/6J
transcript	NM_176953	RefSeq | MGI Sequence Detail	4081	C57BL/6
polypeptide	Q8BTF7	UniProt | EBI | MGI Sequence Detail	911	Not Applicable

All sequences(25) RefSeq(2) UniProt(2)

Polymorphisms

SNPs(8 from dbSNP Build 128)

Protein-related information

Resource	ID	Description
InterPro	IPR001357	BRCT domain
InterPro	IPR000977	DNA ligase, ATP-dependent
InterPro	IPR012310	DNA ligase, ATP-dependent, central
InterPro	IPR016059	DNA ligase, ATP-dependent, conserved site
InterPro	IPR012309	DNA ligase, ATP-dependent, C-terminal
InterPro	IPR012308	DNA ligase, ATP-dependent, N-terminal
InterPro	IPR021536	DNA ligase IV
InterPro	IPR012340	Nucleic acid-binding, OB-fold
Protein Ontology	PR:000009802	DNA ligase 4

Graphical View of Protein Domain Structure

References

(Earliest) J:50865 Frank KM, et al., Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV. Nature. 1998 Nov 12;396(6707):173-7
(Latest) J:194338 Lottersberger F, et al., Role of 53BP1 oligomerization in regulating double-strand break repair. Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):2146-51
All references(88)

Other accession IDs

MGI:2442329, MGI:3584400