Pou5f1
Gene Detail

Symbol Name ID

Pou5f1 POU domain, class 5, transcription factor 1 MGI:101893

Synonyms

Oct-3, Oct-3/4, Oct3/4, Oct4, Oct-4, Otf3, Otf-3, Otf3g, Otf3-rs7, Otf4, Otf-4

Feature Type

protein coding gene

Genetic Map

Chromosome 17

18.69 cM
Detailed Genetic Map ± 1 cM


Mapping data(7)

Sequence Map

Chr17:35506037-35510776 bp, + strand From VEGA annotation of GRCm38   4740 bp   ±  kb flank VEGA Genome Browser | Ensembl Genome Browser | UCSC Browser | NCBI Map Viewer

Mouse Genome Browser

Vertebrate homology

HomoloGene:8422  Vertebrate Homology Class
1 human; 1 mouse; 1 rat; 1 rhesus macaque; 1 dog

Protein SuperFamily: transcription factor Oct-3
Gene Tree: Pou5f1

Human homologs

Human Homolog		POU5F1, POU class 5 homeobox 1
NCBI Gene ID		5460
neXtProt AC		NX_Q01860
Human Synonyms		Oct-3, OCT3, Oct-4, OCT4, OTF3, OTF-3, OTF4
Human Chr (Location)		6p21.31; chr6:31132114-31138451 (-)  GRCh37.p10

Alleles and phenotypes

All alleles(86) : Targeted(7) Gene trapped(79)
 

Homozygosity for a targeted null mutation results in peri-implantation lethality prior to the egg cylinder stage, associated with failure to develop a pluripotent inner cell mass. Conditional mutations show defects in reproduction.

 

Gene Ontology (GO) classifications

All GO classifications: (87 annotations)

Process	BMP signaling pathway involved in heart induction, cardiac cell fate determination, ...
Component	cytoplasm, cytosol, ...
Function	chromatin binding, DNA binding, ...

This is a GO Consortium Reference Genome Project gene.
External Resources: FuncBase

Expression

Literature Summary: (297 records)
Data Summary: Results (708)    Tissues (232)    Images (228)
Theiler Stages: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 28

Assay Type	Results
Immunohistochemistry	123
RNA in situ	281
Northern blot	45
Western blot	1
RT-PCR	253
RNase protection	5

cDNA source data(55)
External Resources: Allen Institute   GENSAT   GEO   ArrayExpress

Molecular reagents

All nucleic(115) Genomic(9) cDNA(61) Primer pair(27) Other(18)
Microarray probesets(3)

Other database links

VEGA Gene Model	OTTMUSG00000037235 (Evidence)
Ensembl Gene Model	ENSMUSG00000024406 (Evidence)
Entrez Gene	18999 (Evidence)
UniGene	17031
DFCI	TC1579170
DoTS	DT.492255, DT.91336507
NIA Mouse Gene Index	U017906
PDB	1OCP, 3L1P
Consensus CDS Project	CCDS37600.1
International Mouse Knockout Project Status	Pou5f1

Sequences

Length	Strain/Species
genomic	OTTMUSG00000037235	VEGA Gene Model | MGI Sequence Detail	4740	C57BL/6J
transcript	OTTMUST00000096009	VEGA | MGI Sequence Detail	1347	Not Applicable
polypeptide	OTTMUSP00000053597	VEGA | MGI Sequence Detail	352	Not Applicable

All sequences(70) RefSeq(4) UniProt(5)

Polymorphisms

RFLP(7) : SNPs(17 from dbSNP Build 128)

Protein-related information

Resource	ID	Description
InterPro	IPR017970	Homeobox, conserved site
InterPro	IPR001356	Homeodomain
InterPro	IPR009057	Homeodomain-like
InterPro	IPR010982	Lambda repressor-like, DNA-binding domain
InterPro	IPR013847	POU domain
InterPro	IPR015585	POU domain-containing protein, class 5
InterPro	IPR000327	POU-specific
Protein Ontology	PR:000013044	POU domain, class 5, transcription factor 1

References

(Earliest) J:10293 Okamoto K, et al., A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell. 1990 Feb 9;60(3):461-72
(Latest) J:195192 Fragola G, et al., Cell reprogramming requires silencing of a core subset of polycomb targets. PLoS Genet. 2013 Feb;9(2):e1003292
All references(444)

Other accession IDs

MGD-MRK-12976, MGD-MRK-12977, MGD-MRK-13063, MGD-MRK-13065, MGD-MRK-13070, MGD-MRK-13077, MGD-MRK-13080, MGD-MRK-18720, MGD-MRK-19416