All Phenotypes Sox2<tm4.1Skn> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Sox2^tm2Skn/Sox2^tm4.1Skn Tg(Nes-cre)1Kln/0	either: (involves: 129S/Sv * C57BL/6 * SJL) or (involves: 129S/Sv * C57BL/6 * DBA/2 * SJL)	MGI:4398746
cn2	Rb1^tm3Tyj/Rb1⁺ Sox2^tm4.1Skn/Sox2^tm4.1Skn Trp53^tm1Brn/Trp53^tm1Brn Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129 * C57BL/6 * CD-1	MGI:7484195
cn3	Rb1^tm3Tyj/Rb1⁺ Sox2^tm4.1Skn/Sox2⁺ Trp53^tm1Brn/Trp53^tm1Brn Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0	involves: 129 * C57BL/6 * CD-1	MGI:7484197
cn4	Sox2^tm4.1Skn/Sox2^tm4.1Skn Tbx18^{tm2.1(cre)Sev}/Tbx18⁺	involves: 129S1/Sv	MGI:5461657
cn5	Sox2^tm4.1Skn/Sox2^tm4.1Skn Tg(Sox2-cre/ERT2)1Skn/0	involves: 129S1/Sv * 129S4/SvJae	MGI:4397784

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:154650 )

• most mice die by 4 weeks of age

nervous system

abnormal neuron differentiation ( J:154650 )

• brain cultures of P0 neurospheres in EGF exhibit reduced neural stem cells by the second and fourth passage compared to wild-type cultures that continue to proliferate

• neurospheres from P0 brain cultures in EGF die out by the fifth or sixth passage unlike wild-type cultures that continue to proliferate

• P0 brain cultures in EGF and bFGF adhere to the plate at early passages before becoming exhausted unlike similarly treated wild-type cultures that continue to proliferate

• defects in neural stem cell maintenance are accompanied by a strong decrease in neurosphere size

• P7 brain cultures are exhausted between the first and fourth passage unlike wild-type cultures that continue to proliferate

• brain cultures from E14.5 mice are more viable than later cultures but exhibit reduced proliferation compared with wild-type cultures

• however, treatment of P0 cultures with media from wild-type cultures restores proliferation

abnormal hippocampus development ( J:154650 )

• from P0 hippocampal development is reduced compared to in wild-type mice

enlarged lateral ventricles ( J:154650 )

• moderately at P0

abnormal corpus callosum morphology ( J:154650 )

• prematurely interrupted at P0

abnormal dentate gyrus morphology ( J:154650 )

• at P0, the number of GFAP/nestin+ cells in the dentate gyrus sub-granular layer is slightly decreased compared to in wild-type mice

• at P2, the number of GFAP/nestin+ cells in the dentate gyrus is strongly reduced compared to in wild-type mice

• the reduction in dentate gyrus cells is attributed to decreased proliferation and transient increase in apoptosis compared to in wild-type mice

absent dentate gyrus ( J:154650 )

• almost completely by P7

loss of hippocampal neurons ( J:154650 )

• the reduction in dentate gyrus cells is attributed to decreased proliferation and transient increase in apoptosis compared to in wild-type mice

small hippocampus ( J:154650 )

• slightly at P0 and markedly at P7

abnormal cerebral cortex morphology ( J:154650 )

• at P0, the posterior ventrolateral cortex is slightly reduced in size compared to in wild-type mice

cellular

abnormal neuron differentiation ( J:154650 )

• brain cultures of P0 neurospheres in EGF exhibit reduced neural stem cells by the second and fourth passage compared to wild-type cultures that continue to proliferate

• neurospheres from P0 brain cultures in EGF die out by the fifth or sixth passage unlike wild-type cultures that continue to proliferate

• P0 brain cultures in EGF and bFGF adhere to the plate at early passages before becoming exhausted unlike similarly treated wild-type cultures that continue to proliferate

• defects in neural stem cell maintenance are accompanied by a strong decrease in neurosphere size

• P7 brain cultures are exhausted between the first and fourth passage unlike wild-type cultures that continue to proliferate

• brain cultures from E14.5 mice are more viable than later cultures but exhibit reduced proliferation compared with wild-type cultures

• however, treatment of P0 cultures with media from wild-type cultures restores proliferation

Allelic Composition	Rb1^tm3Tyj/Rb1⁺ Sox2^tm4.1Skn/Sox2^tm4.1Skn Trp53^tm1Brn/Trp53^tm1Brn Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
Genetic Background	involves: 129 * C57BL/6 * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rb1^tm3Tyj mutation (10 available); any Rb1 mutation (106 available)
Sox2^tm4.1Skn mutation (1 available); any Sox2 mutation (56 available)
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc mutation (2 available)
Trp53^tm1Brn mutation (18 available); any Trp53 mutation (232 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

increased tumor-free survival time ( J:318885 )

• tumor free survival time is increased compared to mutant mice wild-type for Sox2

neoplasm

decreased osteosarcoma incidence ( J:318885 )

• compared to mutant mice wild-type for Sox2

• tumors are uniformly Sox2 positive

skeleton

decreased osteosarcoma incidence ( J:318885 )

• compared to mutant mice wild-type for Sox2

• tumors are uniformly Sox2 positive

Allelic Composition	Rb1^tm3Tyj/Rb1⁺ Sox2^tm4.1Skn/Sox2⁺ Trp53^tm1Brn/Trp53^tm1Brn Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
Genetic Background	involves: 129 * C57BL/6 * CD-1

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

increased tumor-free survival time ( J:318885 )

• tumor free survival time is increased compared to mutant mice wild-type for Sox2

neoplasm

decreased osteosarcoma incidence ( J:318885 )

• compared to mutant mice wild-type for Sox2

• tumors are uniformly Sox2 positive

skeleton

decreased osteosarcoma incidence ( J:318885 )

• compared to mutant mice wild-type for Sox2

• tumors are uniformly Sox2 positive

Allelic Composition	Sox2^tm4.1Skn/Sox2^tm4.1Skn Tbx18^{tm2.1(cre)Sev}/Tbx18⁺
Genetic Background	involves: 129S1/Sv

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sox2^tm4.1Skn mutation (1 available); any Sox2 mutation (56 available)
Tbx18^{tm2.1(cre)Sev} mutation (0 available); any Tbx18 mutation (36 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

integument

integument phenotype ( J:191082 )

abnormal hair growth ( J:191082 )

• hair shaft outgrowth is markedly delayed after birth compared to controls (heterozygotes); guard hair shaft lengths are significantly shorter than controls at P8 and later stages, indicating reduced outgrowth speed

• guard and awl/auchene hairs are 20-55% shorter in mutants, while zigzag hairs are comparable to heterozygous controls

• proliferation and differentiation of matrix progenitor cells are normal

• BrdU labeling of matrix cells at P5 indicates that, while proliferation is normal in mutants, the rate of transition into the hair shaft area is delayed; delay in migration is observed up to 48 hours after BrdU injection; upward movement of differentiation matrix progenitor cells is impaired

• migration of hair shaft progenitors in mutant zigzag follicles is much slower than in control guard hairs; migration speed of mutant guard hairs is reduced and becomes comparable to the speed of migration of control or mutant zigzag follicles

Allelic Composition	Sox2^tm4.1Skn/Sox2^tm4.1Skn Tg(Sox2-cre/ERT2)1Skn/0
Genetic Background	involves: 129S1/Sv * 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sox2^tm4.1Skn mutation (1 available); any Sox2 mutation (56 available)
Tg(Sox2-cre/ERT2)1Skn mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal neuron differentiation ( J:154650 )

• following tamoxifen treatment, the numbers of SOX2/GFAP+ cells with radial glial morphology and GFAP/nestin+ cells at the base of the dentate gyrus are reduced 40% compared to in wild-type mice or untreated controls

abnormal radial glial cell morphology ( J:154650 )

abnormal neuronal precursor proliferation ( J:154650 )

• following tamoxifen treatment, proliferation of neuronal precursor cells downstream of stem cells is decreased compared to in wild-type mice or untreated controls

• following tamoxifen treatment, doublecortin+ cells exhibit reduced proliferation compared to in wild-type mice but to a lesser extent than earlier precursors

cellular

abnormal neuron differentiation ( J:154650 )

abnormal radial glial cell morphology ( J:154650 )

abnormal neuronal precursor proliferation ( J:154650 )

• following tamoxifen treatment, proliferation of neuronal precursor cells downstream of stem cells is decreased compared to in wild-type mice or untreated controls

• following tamoxifen treatment, doublecortin+ cells exhibit reduced proliferation compared to in wild-type mice but to a lesser extent than earlier precursors

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