Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bak1tm1Thsn mutation
(2 available);
any
Bak1 mutation
(23 available)
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hematopoietic system
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• platelet cell numbers are about 50% higher than controls
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• platelet half-life is significantly increased by about 50% compared to controls
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homeostasis/metabolism
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bak1tm1Thsn mutation
(2 available);
any
Bak1 mutation
(23 available)
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normal phenotype
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• mutants are developmentally normal, exhibit normal fertility and fail to develop any age-related disorders
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immune system
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• 4-fold increase in the number of CD43+IgM- Pro-B cells
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• relative number of CD21highCD23low marginal zone B cells is decreased
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• increase in the number of B cells at all stages of development in the bone marrow and spleen
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• a 3-5 fold increase in the numbers of transitional B cells
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• an increase in B-2 cell number, but not B-1 cells, from the peritoneal cavity
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• an increase in CD21intCD23high follicular B cells
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• a 4-fold increase in the number of IgM+ immature B cells
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• B cells are highly resistant to multiple cell death stimuli, including cytokine withdrawal, BCR crosslinking, steroid treatment, and DNA damage
• cell cycle progression of splenic B cells is impaired after stimulation with mitogens LPS and anti-IgM, but not CpG-DNA
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• all isotypes were 5-10 times higher than controls
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• increased IgG1, IgG2, IgG2b and IgG3
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hematopoietic system
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• 4-fold increase in the number of CD43+IgM- Pro-B cells
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• 2-fold increase in total cellularity of bone marrow cells, due to accumulation of developing B cells
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• relative number of CD21highCD23low marginal zone B cells is decreased
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• increase in the number of B cells at all stages of development in the bone marrow and spleen
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• a 3-5 fold increase in the numbers of transitional B cells
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• an increase in B-2 cell number, but not B-1 cells, from the peritoneal cavity
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• an increase in CD21intCD23high follicular B cells
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• a 4-fold increase in the number of IgM+ immature B cells
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• B cells are highly resistant to multiple cell death stimuli, including cytokine withdrawal, BCR crosslinking, steroid treatment, and DNA damage
• cell cycle progression of splenic B cells is impaired after stimulation with mitogens LPS and anti-IgM, but not CpG-DNA
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• all isotypes were 5-10 times higher than controls
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• increased IgG1, IgG2, IgG2b and IgG3
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mortality/aging
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• by 35 weeks after induction of Cre expression (and thus Bax deletion) in the adult, 78% of mutants die compared to 5% of wild-type
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immune system
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• thymic T cell development is perturbed after poly(I:C) injection
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• accumulation of white blood cells after injection of poly(I:C) to induce Cre expression in adult
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• exhibit accumulation of B cells in the spleen and bone marrow 6 weeks after poly(I:C) injection to induce Cre expression
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• develop autoimmune disease after poly(I:C) injection to induce Bax deletion in the adult
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• show elevated serum antinuclear antibodies and anti-dsDNA antibody after 30 weeks of poly(I:C) injection to induce Bax deletion in the adult
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• develops after poly(I:C) injection to induce Bax deletion in the adult
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• develops after poly(I:C) injection to induce Bax deletion in the adult
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hematopoietic system
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• thymic T cell development is perturbed after poly(I:C) injection
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• accumulation of white blood cells after injection of poly(I:C) to induce Cre expression in adult
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• exhibit accumulation of B cells in the spleen and bone marrow 6 weeks after poly(I:C) injection to induce Cre expression
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renal/urinary system
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• develops after poly(I:C) injection to induce Bax deletion in the adult
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skeleton
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• develops after poly(I:C) injection to induce Bax deletion in the adult
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neoplasm
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• intranasal instillation of an adenovirus expressing Cre recombinase (Ad-Cre) results in the development of sinonasal adenocarcinomas
• however, mice injected intramuscularly with Ad-Cre into the extremities or uterus do not develop soft tissue sarcomas
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hematopoietic system
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N |
• unlike in Bcl2l1tm1.1Mam/Bcl2l1tm1.1Mam Tg(MMTV-cre)Mam mice or Bnip3ltm1Ney homozygotes, spleen size, red blood cell numbers and reticulocytes clear of mitochondria are normal
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immune system
hematopoietic system
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bak1tm1Thsn mutation
(2 available);
any
Bak1 mutation
(23 available)
Tg(Lck-cre)1Cwi mutation
(3 available)
Vdac2tm1.1Ehyc mutation
(0 available);
any
Vdac2 mutation
(18 available)
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immune system
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N |
• thymus size normal
• thymocyte number normal
• no increase in apoptosis
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immune system
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• completely resistant to apoptosis
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cellular
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• completely resistant to apoptosis
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hematopoietic system
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• completely resistant to apoptosis
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endocrine/exocrine glands
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• completely resistant to apoptosis
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bak1tm1Thsn mutation
(2 available);
any
Bak1 mutation
(23 available)
Bcl2l1tm1Dlo mutation
(0 available);
any
Bcl2l1 mutation
(104 available)
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hematopoietic system
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• platelet cell numbers are about 50% higher than controls
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• platelet half-life is significantly increased by about 50% compared to controls
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homeostasis/metabolism
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bak1tm1Thsn mutation
(2 available);
any
Bak1 mutation
(23 available)
Bcl2l1tm1Dlo mutation
(0 available);
any
Bcl2l1 mutation
(104 available)
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hematopoietic system
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• the low platelet numbers of Bcl2l1tm1Dlo heterozygotes do not occur in these mice
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hematopoietic system
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• the low platelet numbers of Bcl2l1Plt20 heterozygotes do not occur in these mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bak1tm1Thsn mutation
(2 available);
any
Bak1 mutation
(23 available)
Hax1tm1Jni mutation
(0 available);
any
Hax1 mutation
(36 available)
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mortality/aging
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• survival reduced relative to controls but improved relative to Hax1tm1Jni single homozygous mice
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mortality/aging
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• majority die within 48 hours of birth, although some survive to adulthood
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limbs/digits/tail
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• mutants retain interdigital webs on both fore and rear paws
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reproductive system
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• seen in all adult females
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behavior/neurological
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• display circling behavior when exposed to external stress
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• stress-induced seizure activity
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hearing/vestibular/ear
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• unresponsive to auditory stimuli
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hematopoietic system
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• hematopoietic colony assays show an increase in the number of myeloid colony forming units and a mild increase in erythroid and megakaryocyte colony forming units
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• increases within the circulation and the peripheral lymphoid organs
• lymphocytic infiltration is seen in parenchymal organs, liver, and kidney
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• B220+ B cells present in the lymph node and spleen are skewed toward a B220brightIgD- phenotype, indicating an increase in the number of class-switched or memory B cells
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• T cells that accumulate in mutants are skewed toward a memory cell phenotype
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• expanded red pulp contains a large increase in the number of plasma cells and histiocytes
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• isolated splenocytes cultured in suspension show enhanced survival
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immune system
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• increases within the circulation and the peripheral lymphoid organs
• lymphocytic infiltration is seen in parenchymal organs, liver, and kidney
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• B220+ B cells present in the lymph node and spleen are skewed toward a B220brightIgD- phenotype, indicating an increase in the number of class-switched or memory B cells
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• T cells that accumulate in mutants are skewed toward a memory cell phenotype
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• expanded red pulp contains a large increase in the number of plasma cells and histiocytes
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• isolated splenocytes cultured in suspension show enhanced survival
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nervous system
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• stress-induced seizure activity
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• increase in the number of neurons in multiple regions of the brain
• large accumulation of small neuronal cells (neural stem cells) with dense chromatin staining in the periventricular region
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• in multiple regions of the brain
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cellular
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• thymocytes show enhanced survival compared to wild-type when exposed to gamma irradiation
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homeostasis/metabolism
growth/size/body
hematopoietic system
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N |
• unlike in Bnip3ltm1Ney homozygotes, reticulocytes clearance of mitochondria is normal
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Analysis Tools