Kras^tm4Tyj
Targeted Allele Detail

Summary

• Symbol: Kras^tm4Tyj
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 4, Tyler Jacks
• MGI ID: MGI:2429948
• Synonyms: caKRas, KR, Kras2tm14Tyj, Kras2^tm4Tyj, Kras^G12D, K-RasG12D, K-Ras(G12D)^fl, K-Ras^L, Kras^Lox, K-ras^LSL, Kras^LSL-G12D, Lox-Stop-Lox-Kras^G12D, LSL-KrasG12D, LSL-K-ras^G12D, LSL-K-ras G12D, LSL-Kras^G12D, LSL-Kras G12D
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm4Tyj page

Pancreatic tumors in Kras^tm4Tyj/Kras^tm4Tyj Trim33^tm1Los/Trim33^tm1Los Tg(Pdx1-cre)89.1Dam/0 and Kras^tm4Tyj/Kras^tm4Tyj Cdkn2a^tm4Rdp/Cdkn2a^tm4Rdp Tg(Pdx1-cre)89.1Dam/0 mice

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73445
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: By homologous recombination in ES cells, the Kras2 locus was targeted with a cassette containing an oncogenic form of the KRAS2 protein in which the glycine at position 12 had been substituted with a an aspartic acid. A loxP flanked stop codon was included upstream of the inserted Kras2 sequence, such that the mutant transcript would be expressed only after cre-mediated recombination. (J:73445)

Disease models

Expression

In Mice Carrying this Mutation:	67 assay results
In Structures Affected by this Mutation:	54 anatomical structures

Tumor Data

List all tumor models in MMHCdb carrying Kras^tm4Tyj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 9 strains available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 54 strains or lines available

References

• Original: J:73445 Jackson EL, et al., Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Genes Dev. 2001 Dec 15;15(24):3243-8
• All: 1328 reference(s)