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Gene Expression Data
Assay Details
Assay
Reference: J:308820 Shohayeb B, et al., The Spindle-Associated Microcephaly Protein, WDR62, Is Required for Neurogenesis and Development of the Hippocampus. Front Cell Dev Biol. 2020;8:549353
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7834522
Gene symbol: Tbr1
Gene name: T-box brain transcription factor 1
Antibody: anti-Tbr1 (AB31940)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3A: embryonic day 17.5 (more )
Note: Triple labeled: magenta - Tbr1; green - Eomes (assay MGI:7834523); red - Pax6 (assay MGI:7834524).
Structure Cell Type Level Pattern Image Note
TS25: dentate gyrus glutamatergic neuron Present 3A , 3A"'
TS25: hippocampus glutamatergic neuron Present Regionally restricted 3A" , 3A Expressed in the hippocampal cornus ammonis region.
Specimen 3B: embryonic day 17.5; Wdr62em3Dng/Wdr62em3Dng (more )
Note: Triple labeled: magenta - Tbr1; green - Eomes (assay MGI:7834523); red - Pax6 (assay MGI:7834524).
Structure Cell Type Level Pattern Image Note
TS25: dentate gyrus glutamatergic neuron Present 3B"' , 3B Reduced expression compared with the wild-type.
TS25: hippocampus glutamatergic neuron Present Regionally restricted 3B , 3B" Reduced expression in the hippocampal cornus ammonis region.
Specimen 3C: embryonic day 17.5; Wdr62em1Dng/Wdr62em1Dng (more )
Note: Triple labeled: magenta - Tbr1; green - Eomes (assay MGI:7834523); red - Pax6 (assay MGI:7834524).
Structure Cell Type Level Pattern Image Note
TS25: dentate gyrus glutamatergic neuron Present 3C"' , 3C Reduced expression compared with the wild-type.
TS25: hippocampus glutamatergic neuron Present Regionally restricted 3C , 3C" Reduced expression in the hippocampal cornus ammonis region, more severe than that of WDR62stop mutant.
Specimen 3D: embryonic day 17.5; Wdr62em2Dng/Wdr62em2Dng (more )
Note: Triple labeled: magenta - Tbr1; green - Eomes (assay MGI:7834523); red - Pax6 (assay MGI:7834524).
Structure Cell Type Level Pattern Image Note
TS25: dentate gyrus glutamatergic neuron Present 3D , 3D"' Reduced expression compared with the wild-type.
TS25: hippocampus glutamatergic neuron Present Regionally restricted 3D , 3D" Reduced expression in the hippocampal cornus ammonis region, more severe than that of WDR62stop mutant.
Specimen S3A: embryonic day 15.5 (more )
Note: Triple labeled: magenta - Tbr1; green - Eomes (assay MGI:7834523); red - Pax6 (assay MGI:7834524).
Structure Cell Type Level Pattern Image Note
TS23: dentate gyrus primordium glutamatergic neuron Present S3A" , S3A
Specimen S3B: embryonic day 15.5; Wdr62em3Dng/Wdr62em3Dng (more )
Note: Triple labeled: magenta - Tbr1; green - Eomes (assay MGI:7834523); red - Pax6 (assay MGI:7834524).
Structure Cell Type Level Pattern Image Note
TS23: dentate gyrus primordium glutamatergic neuron Present S3B , S3B" Reduced expression compared with the wild-type.
Specimen S3C: embryonic day 15.5; Wdr62em1Dng/Wdr62em1Dng (more )
Note: Triple labeled: magenta - Tbr1; green - Eomes (assay MGI:7834523); red - Pax6 (assay MGI:7834524).
Structure Cell Type Level Pattern Image Note
TS23: dentate gyrus primordium glutamatergic neuron Present S3C , S3C" Reduced expression compared with the wild-type.
Specimen S3D: embryonic day 15.5; Wdr62em2Dng/Wdr62em2Dng (more )
Note: Triple labeled: magenta - Tbr1; green - Eomes (assay MGI:7834523); red - Pax6 (assay MGI:7834524).
Structure Cell Type Level Pattern Image Note
TS23: dentate gyrus primordium glutamatergic neuron Present S3D" , S3D Reduced expression compared with the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory