Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  1
• Reference
  J:70272 Kibar Z, et al., Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail. Nat Genet. 2001 Jul;28(3):251-5
• ID
  MGI:2137382

Genes

Gene	Allele	Assay Type
D1Mit113		overlapping clones, direct sequencing, sequence analysis
Cd84		overlapping clones, direct sequencing, sequence analysis
Vangl2	Vangl2^Lp	overlapping clones, direct sequencing, sequence analysis
Nhlh1		overlapping clones, direct sequencing, sequence analysis
Ncstn		overlapping clones, direct sequencing, sequence analysis
Copa		overlapping clones, direct sequencing, sequence analysis
Pex19		overlapping clones, direct sequencing, sequence analysis
Timm23		overlapping clones, direct sequencing, sequence analysis
Dcaf8		overlapping clones, direct sequencing, sequence analysis
Pea15a		overlapping clones, direct sequencing, sequence analysis
Casq1		overlapping clones, direct sequencing, sequence analysis
Atp1a4		overlapping clones, direct sequencing, sequence analysis
Atp1a2		overlapping clones, direct sequencing, sequence analysis
Igsf8		overlapping clones, direct sequencing, sequence analysis
Kcnj9		overlapping clones, direct sequencing, sequence analysis
Kcnj10		overlapping clones, direct sequencing, sequence analysis
Pigm		overlapping clones, direct sequencing, sequence analysis
Igsf9		overlapping clones, direct sequencing, sequence analysis
Tagln2		overlapping clones, direct sequencing, sequence analysis
Fcer1a		overlapping clones, direct sequencing, sequence analysis

Notes

• Experiment
  The authors used positional cloning and a transcription mapping approach to identify genes in the 0.7 Mb Lp region. The order of markers in the interval was determined to be: centromere - D1Mit113 - Cd84 - Vangl2 - Nhlh1 - Ncstn - Copa - Pex19 - Timm23 -AA792214 - Pea15 - Casq1 - Atp1a4 - Atp1a2 - Igsf8 - Kcnj9 - Kcnj10 - Pigm - 644ETD8 - Tagln2 - Fcer1a - telomere. The Ltap gene was altered by a single base change in two different Lp alleles. These changes were not observed in inbred strains and more distant species of wild origin. Confirmation that Lp and Vangl2 are allelic will have to be performed using genetic complementation with trangenic mice or creation of a null mutation at the locus.