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Gene Expression Data
Assay Details
Assay
Reference: J:290119 Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8270443
Gene symbol: Th
Gene name: tyrosine hydroxylase
Antibody: anti-TH (rabbit; P40101-150)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present). Two Th antibodies were listed MGI:6159135 and MGI:4460872; the one used in this assay was not specified.
Results
Specimen 1k: embryonic day 18.5 (more )
Note: Double labeled: red - Th; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: hypothalamus Present Regionally restricted 1k Expression was detected in dopaminergic axons.
Specimen 1l: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Th; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: hypothalamus Present Regionally restricted 1l Mainly Slc6a3-positive;TH-positive substantia nigra dopaminergic axon bundles cross the midline.
Specimen 1m: embryonic day 18.5 (more )
Note: Double labeled: red - Th; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted 1m Expression was detected in dopaminergic axons.
Specimen 1n: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Th; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: brain Present Regionally restricted 1n Slc6a3-negative;TH-positive dopaminergic axons extend towards the striatum.
Specimen 1o: embryonic day 18.5 (more )
Note: Double labeled: red - Th; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: midbrain Present Regionally restricted 1o Expression was detected in dopaminergic axons.
TS26: diencephalon Present Regionally restricted 1o Expression was detected in dopaminergic axons in caudal diencephalon.
Specimen 1p: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Th; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: diencephalon Present Regionally restricted 1p The immunolabeled dopaminergic axon bundle is defasciculated and more spread out along the medial-lateral axis in Nolz1-/- mutant embryos
TS26: midbrain Present Regionally restricted 1p The immunolabeled dopaminergic axon bundle is defasciculated and more spread out along the medial-lateral axis in Nolz1-/- mutant embryos
Specimen 6i: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: corpus striatum Present Regionally restricted 6i Expression was detected in dopaminergic axons.
Specimen 6j: embryonic day 14.5; Pcdh10tm1Lex/Pcdh10tm1Lex (more )
Structure Level Pattern Image Note
TS22: corpus striatum Present Regionally restricted 6j Innervation of the striatum by dopaminergic axon terminals was not affected in Pcdh10-/- embryos.
Specimen S1c WT (with GlycoDAT): embryonic day 18.5 (more )
Note: Double labeled: red - Th; green - Slc6a3 (assay MGI:8270447).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c WT GlycoDAT TH
Specimen S1c Nolz1bGal/bGal (with GlycoDAT): embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Th; green - Slc6a3 (assay MGI:8270447).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c Nolz1bgal/bgal GlycoDAT TH
Specimen S1c WT (with Aldh1a1): embryonic day 18.5 (more )
Note: Double labeled: red - Th; green - Aldh1a1 (assay MGI:8270719).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c WT ALDH1A1 TH
Specimen S1c Nolz1bGal/bGal (with Aldh1a1): embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Th; green - Aldh1a1 (assay MGI:8270719).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c Nolz1bgal/bgal ALDH1A1 TH
Specimen S1c WT (with Otx2): embryonic day 18.5 (more )
Note: Double labeled: red - Th; green - Otx2 (assay MGI:8270718).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c WT Otx2 TH
Specimen S1c Nolz1bGal/bGal (with Otx2): embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Th; green - Otx2 (assay MGI:8270718).
Structure Cell Type Level Pattern Image Note
TS26: midbrain midbrain dopaminergic neuron Present S1c Nolz1bgal/bgal Otx2 TH
Specimen S5f WT: postnatal year 1 (more )
Structure Level Pattern Image Note
TS28: accumbens nucleus Present S5f WT
Specimen S5f En1Cre;Nolz1fl/fl: postnatal year 1; En1tm2(cre)Wrst/En1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: accumbens nucleus Present S5f En1Cre;Nolz1fl/fl The fiber density of the nucleus accumbens was not changed in En1Cre;Nolz1<fl/fl> adults.
Specimen S6g: embryonic day 18.5 (more )
Note: Double labeled: red - Th; green - L1cam (assay MGI:8270624).
Structure Level Pattern Image Note
TS26: corpus striatum Present Regionally restricted S6g Expression was detected in dopaminergic axons.
Specimen S6h: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Th; green - L1cam (assay MGI:8270624).
Structure Level Pattern Image Note
TS26: corpus striatum Ambiguous S6h Dopaminergic axons are misguided in Nolz1-/- mutant embryos.
Specimen S6i: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: red - Th; green - L1cam (assay MGI:8270624).
Structure Level Pattern Image Note
TS26: corpus striatum Weak Regionally restricted S6i There was a strong reduction in striatal innervation by dopaminergic terminals in Foxg1IresCre;Nolz1<fl/fl> embryos, although to a lesser extent compared with Nolz1-/- mutant embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
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