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Gene Expression Data
Assay Details
Assay
Reference: J:336175 Fuchs MAA, et al., Intact prostaglandin signaling through EP2 and EP4 receptors in stromal progenitor cells is required for normal development of the renal cortex in mice. Am J Physiol Renal Physiol. 2022 Mar 1;322(3):F295-F307
Assay type: RNA in situ
MGI Accession ID: MGI:7531043
Gene symbol: Pdgfrb
Gene name: platelet derived growth factor receptor, beta polypeptide
Probe: Mm-Pdgfrb (411381)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope assay.
Results
Specimen 2A (middle): postnatal day 9 (more )
Note: Double labeled: green - Pdgfrb; red - Ptger2 (assay MGI:7531039).
Structure Cell Type Level Pattern Image Note
TS28: glomerular mesangium glomerular mesangial cell Present 2A (middle)
TS28: extraglomerular mesangium extraglomerular mesangial cell Present 2A (middle)
TS28: renal cortical interstitium kidney cortex interstitial cell Present 2A (middle)
Specimen 2B (middle): postnatal day 9 (more )
Note: Double labeled: green - Pdgfrb; red - Ptger4 (assay MGI:7531041).
Structure Cell Type Level Pattern Image Note
TS28: glomerular mesangium glomerular mesangial cell Present 2B (middle)
TS28: extraglomerular mesangium extraglomerular mesangial cell Present 2B (middle)
TS28: renal cortical interstitium kidney cortex interstitial cell Present 2B (middle)
Specimen 2C (middle): postnatal day 9 (more )
Note: Double labeled: green - Pdgfrb; red - Ptgs2 (assay MGI:7531044).
Structure Cell Type Level Pattern Image Note
TS28: glomerular mesangium glomerular mesangial cell Present 2C (middle)
TS28: extraglomerular mesangium extraglomerular mesangial cell Present 2C (middle)
TS28: renal cortical interstitium kidney cortex interstitial cell Present 2C (middle)
Specimen 2A (bottom): postnatal day 9 (more )
Note: Double labeled: green - Pdgfrb; red - Ptger2 (assay MGI:7531039).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present 2A (bottom)
Specimen 2B (bottom): postnatal day 9 (more )
Note: Double labeled: green - Pdgfrb; red - Ptger4 (assay MGI:7531041).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present 2B (bottom)
Specimen 2C (bottom): postnatal day 9 (more )
Note: Double labeled: green - Pdgfrb; red - Ptgs2 (assay MGI:7531044).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present 2C (bottom)
Specimen S2: postnatal day 9 (more )
Note: Triple labeled: green - Pdgfrb; blue - Nphs1 (assay MGI:7531045); red - Ptger4 (assay MGI:7531041).
Structure Cell Type Level Pattern Image Note
TS28: mesangium mesangial cell Present S2_(right) , S2_(left) Colocalization with Ptger4 expression in mesangial cells.
TS28: renal cortical interstitium kidney cortex interstitial cell Present Regionally restricted S2_(left) , S2_(right) Expression in some interstitial cells.
TS28: renal cortex metanephric podocyte Absent S2_(right) , S2_(left) No coexpression with Nphs1 in podocytes.
Specimen 4 cortex (left): postnatal day 9 (more )
Note: Triple labeled: green - Pdgfrb; red - Aqp2 (assay MGI:7531046); blue - Ptger1 (assay MGI:7531038).
Structure Cell Type Level Pattern Image Note
TS28: renal cortical interstitium kidney cortex interstitial cell Present 4_cortex (left)
Specimen 4 cortex (right): postnatal day 9 (more )
Note: Triple labeled: green - Pdgfrb; red - Aqp2 (assay MGI:7531046); blue - Ptger3 (assay MGI:7531040).
Structure Cell Type Level Pattern Image Note
TS28: renal cortical interstitium kidney cortex interstitial cell Present 4_cortex (right)
Specimen 4 inner medulla (left): postnatal day 9 (more )
Note: Triple labeled: green - Pdgfrb; red - Aqp2 (assay MGI:7531046); blue - Ptger1 (assay MGI:7531038).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present 4_inner medulla (left)
Specimen 5A (top): postnatal week 15 (more )
Note: Double labeled: green - Pdgfrb; red - Ptger2 (assay MGI:7531039).
Structure Level Pattern Image Note
TS28: macula densa Absent 5A (top)
Specimen 5A (bottom): postnatal week 15 (more )
Note: Double labeled: green - Pdgfrb; red - Ptger2 (assay MGI:7531039).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present 5A (bottom)
Specimen 5B (top): postnatal week 15 (more )
Note: Double labeled: green - Pdgfrb; red - Ptger4 (assay MGI:7531041).
Structure Level Pattern Image Note
TS28: macula densa Absent 5B (top)
Specimen 5B (bottom): postnatal week 15 (more )
Note: Double labeled: green - Pdgfrb; red - Ptger4 (assay MGI:7531041).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present 5B (bottom)
Specimen 5C (top): postnatal week 15 (more )
Note: Double labeled: green - Pdgfrb; red - Ptgs2 (assay MGI:7531044).
Structure Level Pattern Image Note
TS28: macula densa Absent 5C (top)
Specimen 5C (bottom): postnatal week 15 (more )
Note: Double labeled: green - Pdgfrb; red - Ptgs2 (assay MGI:7531044).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present 5C (bottom)
Specimen 9 Wildtype: postnatal adult (more )
Note: Double labeled: green - Pdgfrb; red - Col1a1 (assay MGI:7531050).
Structure Level Pattern Image Note
TS28: renal cortex Present 9_Wildtype
Specimen 9 FoxD1+/Cre EP2-/- EP4f/f: postnatal adult; Foxd1tm1(GFP/cre)Amc/Foxd1+, Ptger2tm1Brey/Ptger2tm1Brey, Ptger4tm1.1Matb/Ptger4tm1.1Matb (more )
Note: Conditional mutant. Double labeled: green - Pdgfrb; red - Col1a1 (assay MGI:7531050).
Structure Level Pattern Image Note
TS28: renal cortex Present 9_FoxD1+/Cre EP2-/- EP4f/f
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory