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Gene Expression Data
Assay Details
Assay
Reference: J:336175 Fuchs MAA, et al., Intact prostaglandin signaling through EP2 and EP4 receptors in stromal progenitor cells is required for normal development of the renal cortex in mice. Am J Physiol Renal Physiol. 2022 Mar 1;322(3):F295-F307
Assay type: RNA in situ
MGI Accession ID: MGI:7531041
Gene symbol: Ptger4
Gene name: prostaglandin E receptor 4 (subtype EP4)
Probe: Mm-Ptger4 (441461)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope assay.
Results
Specimen 2B (top): postnatal day 9 (more )
Note: Double labeled: red - Ptger4; green - Ren1 (assay MGI:7531042).
Structure Cell Type Level Pattern Image Note
TS28: glomerular tuft kidney granular cell Strong Regionally restricted 2B (top) Strong expression in renin-expressing cells at glomeruli.
Specimen 2B (middle): postnatal day 9 (more )
Note: Double labeled: red - Ptger4; green - Pdgfrb (assay MGI:7531043).
Structure Cell Type Level Pattern Image Note
TS28: glomerular mesangium glomerular mesangial cell Present 2B (middle) Expression in mesangial cells more pronounced that for Ptger2.
TS28: renal cortical interstitium kidney cortex interstitial cell Present 2B (middle)
Specimen 2B (bottom): postnatal day 9 (more )
Note: Double labeled: red - Ptger4; green - Pdgfrb (assay MGI:7531043).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present 2B (bottom)
Specimen S2: postnatal day 9 (more )
Note: Triple labeled: red - Ptger4; blue - Nphs1 (assay MGI:7531045); green - Pdgfrb (assay MGI:7531043).
Structure Cell Type Level Pattern Image Note
TS28: glomerular mesangium glomerular mesangial cell Present S2_(left) , S2_(right) , S2_(middle) Strong colocalization with Pdgfrb expression in mesangial cells.
TS28: renal cortical interstitium kidney cortex interstitial cell Present S2_(middle) , S2_(left) , S2_(right) Expression in some interstitial cells.
TS28: renal cortex metanephric podocyte Present S2_(left) , S2_(right) , S2_(middle) Expression in some podocytes.
Specimen S3 cortex: postnatal adult (more )
Note: Double labeled: red - Ptger4; green - Myh11 (assay MGI:7531048).
Structure Cell Type Level Pattern Image Note
TS28: glomerular tuft mesangial cell Present S3_cortex Expression in mesangial cells and podocytes of glomeruli.
TS28: glomerular tuft metanephric podocyte Present S3_cortex Expression in mesangial cells and podocytes of glomeruli.
TS28: renal cortex kidney granular cell Present S3_cortex Expression in renin-expressing cells.
TS28: renal cortical vasculature pericyte Present S3_cortex Expression in some pericytes associated with a renal vessel.
TS28: afferent arteriole of mature renal corpuscle Absent S3_cortex No expression in efferent of afferent arterioles of glomerulus.
TS28: efferent arteriole of mature renal corpuscle Absent S3_cortex No expression in efferent of afferent arterioles of glomerulus.
Specimen S3 inner medulla: postnatal adult (more )
Note: Double labeled: red - Ptger4; green - Pecam1 (assay MGI:7531049).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary vasculature endothelial cell Absent S3_inner medulla No overlap with Pecam1 expression in endothelial cells.
Specimen 3 (right): postnatal day 9 (more )
Note: Double labeled: red - Ptger4; blue - Aqp2 (assay MGI:7531046).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present Regionally restricted 3_(right) Expression in interstitial cells associated with Aqp2-expressing tubules.
Specimen 5B (top): postnatal week 15 (more )
Note: Double labeled: red - Ptger4; green - Pdgfrb (assay MGI:7531043).
Structure Cell Type Level Pattern Image Note
TS28: glomerular mesangium glomerular mesangial cell Present 5B (top)
TS28: extraglomerular mesangium extraglomerular mesangial cell Present 5B (top)
TS28: renal cortical interstitium kidney cortex interstitial cell Present Regionally restricted 5B (top) Expression in some interstitial cells.
Specimen 5B (bottom): postnatal week 15 (more )
Note: Double labeled: red - Ptger4; green - Pdgfrb (assay MGI:7531043).
Structure Cell Type Level Pattern Image Note
TS28: renal medullary interstitium kidney inner medulla interstitial cell Present 5B (bottom)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory