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Gene Expression Data
Assay Details
Assay
Reference: J:264612 Morgani SM, et al., A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice. Dev Biol. 2018 Sep 1;441(1):104-126
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6286387
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: Anti-Nanog
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were described for this gene product; the one used in this assay was not detailed. Either MGI:6286289 or MGI:6241547 could have been used.
Results
Specimen 3A 16c: embryonic day 3.0; Spry4tm1Hadj/Spry4+ (more )
Note: 16 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS3: compacted morula Present 3A 16c Nanog , 3A 16c Merge + Nuclei
Specimen 3A 31c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 31 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS4: compacted morula Present 3A 31c Nanog , 3A 31c Merge + Nuclei
Specimen 3A 59c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 59 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3A 59c Merge + Nuclei , 3A 59c Nanog
Specimen 3A 89c: embryonic day 3.75; Spry4tm1Hadj/Spry4+ (more )
Note: 89 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3A 89c Nanog , 3A 89c Merge + Nuclei
Specimen 3A 106c: embryonic day 4.0; Spry4tm1Hadj/Spry4+ (more )
Note: 106 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A 106c Merge + Nuclei , 3A 106c Nanog
Specimen 3A 148c: embryonic day 4.5; Spry4tm1Hadj/Spry4+ (more )
Note: 148 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A 148c Nanog , 3A 148c Merge + Nuclei
Specimen 3A 187c: embryonic day 5.0; Spry4tm1Hadj/Spry4+ (more )
Note: 187 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286316).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3A 187c Merge + Nuclei , 3A 187c Nanog
Specimen 3B 15c: embryonic day 3.0; Spry4tm1Hadj/Spry4+ (more )
Note: 15 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS3: compacted morula Ambiguous 3B 15c Nanog , 3B 15c Merge + Nuclei
Specimen 3B 30c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 30 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: compacted morula Present 3B 30c Merge + Nuclei , 3B 30c Nanog
Specimen 3B 57c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 57 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3B 57c Nanog , 3B 57c Merge + Nuclei
Specimen 3B 86c: embryonic day 3.75; Spry4tm1Hadj/Spry4+ (more )
Note: 86 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3B 86c Merge + Nuclei , 3B 86c Nanog
Specimen 3B 91c: embryonic day 4.0; Spry4tm1Hadj/Spry4+ (more )
Note: 91 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B 91c Merge + Nuclei , 3B 91c Nanog
Specimen 3B 141c: embryonic day 4.5; Spry4tm1Hadj/Spry4+ (more )
Note: 141 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B 141c Nanog , 3B 141c Merge + Nuclei
Specimen 3B 159c: embryonic day 5.0; Spry4tm1Hadj/Spry4+ (more )
Note: 159 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 3B 159c Merge + Nuclei , 3B 159c Nanog
Specimen 4A 58c: embryonic day 3.5; Spry4tm1Hadj/Spry4+ (more )
Note: 58 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4A 58c Nanog , 4A 58c Merge + Nuclei
Specimen 4A 62c: embryonic day 3.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt, Spry4tm1Hadj/Spry4+ (more )
Note: 62 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4A 62c Fgf4-/- Nanog , 4A 62c Fgf4-/- Merge + Nuclei
Specimen 4A 62c Fgfr1-/-: embryonic day 3.5; Fgfr1tm1Jrt/Fgfr1tm1Jrt, Spry4tm1Hadj/Spry4+ (more )
Note: 62 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4A 62c Fgfr1-/- Merge + Nuclei , 4A 62c Fgfr1-/- Nanog
Specimen 4A 38c: embryonic day 3.5; Fgfr2tm1Dor/Fgfr2tm1Dor, Spry4tm1Hadj/Spry4+, Tg(CAG-cre)13Miya/0 (more )
Note: Conditional mutant. 38 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4A 38c Fgfr2-/- Nanog , 4A 38c Fgfr2-/- Merge + Nuclei
Specimen 4B 74c: embryonic day 3.75; Spry4tm1Hadj/Spry4+ (more )
Note: 74 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4B 74c Merge + Nuclei , 4B 74c Nanog
Specimen 4B 68c: embryonic day 3.75; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt, Spry4tm1Hadj/Spry4+ (more )
Note: 68 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Ambiguous 4B 68c Merge + Nuclei , 4B 68c Nanog
Specimen 4B 83c: embryonic day 3.75; Fgfr1tm1Jrt/Fgfr1tm1Jrt, Spry4tm1Hadj/Spry4+ (more )
Note: 83 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Ambiguous 4B 83c Nanog , 4B 83c Merge + Nuclei
Specimen 4B 77c: embryonic day 3.75; Fgfr2tm1Dor/Fgfr2tm1Dor, Spry4tm1Hadj/Spry4+, Tg(CAG-cre)13Miya/0 (more )
Note: Conditional mutant. 77 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS4: inner cell mass Present 4B 77c Merge + Nuclei , 4B 77c Nanog
Specimen 4C 115c: embryonic day 4.0; Spry4tm1Hadj/Spry4+ (more )
Note: 115 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 4C 115c Nanog , 4C 115c Merge + Nuclei
Specimen 4C 111c: embryonic day 4.0; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt, Spry4tm1Hadj/Spry4+ (more )
Note: 111 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Ambiguous 4C 111c Merge + Nuclei , 4C 111c Nanog
Specimen 4C 98c: embryonic day 4.0; Fgfr1tm1Jrt/Fgfr1tm1Jrt, Spry4tm1Hadj/Spry4+ (more )
Note: 98 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Ambiguous 4C 98c Nanog , 4C 98c Merge + Nuclei
Specimen 4C 104c: embryonic day 4.0; Fgfr2tm1Dor/Fgfr2tm1Dor, Spry4tm1Hadj/Spry4+, Tg(CAG-cre)13Miya/0 (more )
Note: Conditional mutant. 104 cells. Triple labeled: red - Nanog; blue - Gata6 (assay MGI:6286386); green - Spry4 (assay MGI:6286338).
Structure Level Pattern Image Note
TS5: inner cell mass Present 4C 104c Merge + Nuclei , 4C 104c Nanog
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory