Phenotypes associated with this allele

• Allele Symbol: Polr2m^tm1.1Rgr
• Allele Name: targeted mutation 1.1, Robert G Roeder
• Allele ID: MGI:6503875
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Alb^tm1(cre/ERT2)Mtz/Alb^+ Polr2m^tm1.1Rgr/Polr2m^tm1.1Rgr Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * C57BL/6	MGI:6515759
cn2	Polr2m^tm1.1Rgr/Polr2m^tm1.1Rgr Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+ Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * C57BL/6 * DBA	MGI:6515760
cn3	Polr2m^tm1.1Rgr/Polr2m^tm1.1Rgr Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: C57BL/6 * DBA	MGI:6515758

Genotype
MGI:6515759

cn1

• Allelic Composition: Alb^{tm1(cre/ERT2)Mtz}/Alb⁺; Polr2m^tm1.1Rgr/Polr2m^tm1.1Rgr; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

liver/biliary system

increased hepatocyte karyomegaly ( J:291184 )

• following tamoxifen injection, nuclei are enlarged compared to cells from Trp53 null mice

increased hepatocyte proliferation ( J:291184 )

• expression analysis indicates hepatocytes rapidly reenter the cell cycle after tamoxifen treatment

cellular

increased hepatocyte karyomegaly ( J:291184 )

• following tamoxifen injection, nuclei are enlarged compared to cells from Trp53 null mice

increased hepatocyte proliferation ( J:291184 )

• expression analysis indicates hepatocytes rapidly reenter the cell cycle after tamoxifen treatment

Genotype
MGI:6515760

cn2

• Allelic Composition: Polr2m^tm1.1Rgr/Polr2m^tm1.1Rgr; Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * DBA

liver/biliary system

increased hepatocyte apoptosis ( J:291184 )

• dramatically increased by 8 weeks of age

increased hepatocyte proliferation ( J:291184 )

• expression analysis indicates more cells have re-entered the cell cycle and at 8 weeks of age the number of Ki67 positive hepatocytes is further increased

abnormal liver morphology ( J:291184 )

• liver architecture is distorted with the pericentral expression pattern of glutamine synthetase absent

abnormal hepatocyte morphology ( J:291184 )

• increased size with some being extremely enlarged and containing more than 3 nuclei indicating incomplete mitoses

cellular

increased hepatocyte apoptosis ( J:291184 )

• dramatically increased by 8 weeks of age

increased hepatocyte proliferation ( J:291184 )

• expression analysis indicates more cells have re-entered the cell cycle and at 8 weeks of age the number of Ki67 positive hepatocytes is further increased

Genotype
MGI:6515758

cn3

• Allelic Composition: Polr2m^tm1.1Rgr/Polr2m^tm1.1Rgr; Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
• Genetic Background: involves: C57BL/6 * DBA

liver/biliary system

liver/biliary system phenotype ( J:291184 )

N • liver weights are similar to controls

increased hepatocyte karyomegaly ( J:291184 )

• large cells with enlarged nuclei are seen

abnormal hepatocyte physiology ( J:291184 )

• expression analysis indicates more cells have re-entered the cell cycle but that the cell cycle appears to arrest

homeostasis/metabolism

decreased circulating triglyceride level ( J:291184 )

increased circulating alkaline phosphatase level ( J:291184 )

cellular

increased hepatocyte karyomegaly ( J:291184 )

• large cells with enlarged nuclei are seen