Albtm1(cre/ERT2)Mtz
Targeted Allele Detail
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Symbol: |
Albtm1(cre/ERT2)Mtz |
Name: |
albumin; targeted mutation 1, Daniel Metzger |
MGI ID: |
MGI:3052812 |
Synonyms: |
Alb-CreERT2, AlbCre-ERT2ki, AlbICE, Albtm1(cre/ESR1)Mtz, Albumin-CreER, SACre-ERT2, SA-CRE-ERT2 |
Gene: |
Alb Location: Chr5:90608756-90624461 bp, + strand Genetic Position: Chr5, 44.7 cM
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Alliance: |
Albtm1(cre/ERT2)Mtz page
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Germline Transmission: |
Earliest citation of germline transmission:
J:92298
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
129S2/SvPas
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Albtm1(cre/ERT2)Mtz expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Alb (MGI:87991) |
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Mutation details: A vector was used to insert IRES-Cre-ERT2 downstream of the stop codon in exon 14. Transient FLP-recombinase excised a FRT-floxed neo 3' to the Cre. ES cell clone XF136.64 was injected into blastocysts to generated mice. Southern blot confirmed recombination. RT-PCR indicated bicistronic expression was only present in the liver of mutants. Immunohistochemical analysis of liver using an anti-Cre antibody showed that Cre is expressed in most, if not all, hepatocytes.
(J:92298)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Alb
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:92298 Schuler M, et al., Efficient temporally controlled targeted somatic mutagenesis in hepatocytes of the mouse. Genesis. 2004 Jul;39(3):167-72 |
All: |
100 reference(s) |
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