Phenotypes associated with this allele

• Allele Symbol: Lpcat3^{tm1c(EUCOMM)Wtsi}
• Allele Name: targeted mutation 1c, Wellcome Trust Sanger Institute
• Allele ID: MGI:5822780
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lpcat3^tm1c(EUCOMM)Wtsi/Lpcat3^tm1c(EUCOMM)Wtsi	involves: C57BL/6 * C57BL/6N * SJL	MGI:5822851
cn2	Lpcat3^tm1c(EUCOMM)Wtsi/Lpcat3^tm1c(EUCOMM)Wtsi Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: C57BL/6 * C57BL/6N * SJL	MGI:5822858
cn3	Lpcat3^tm1c(EUCOMM)Wtsi/Lpcat3^tm1c(EUCOMM)Wtsi Tg(Vil1-cre)997Gum/0	involves: C57BL/6 * C57BL/6N * SJL	MGI:5822876

Genotype
MGI:5822851

hm1

• Allelic Composition: Lpcat3^{tm1c(EUCOMM)Wtsi}/Lpcat3^{tm1c(EUCOMM)Wtsi}
• Genetic Background: involves: C57BL/6 * C57BL/6N * SJL
• Cell Lines: EPD0455_5_A07

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased circulating triglyceride level ( J:220827 )

• at 3 weeks after injection with a Cre-expressing adenoviral vector, adult mice show a more pronounced decrease in fasting plasma TG levels relative to mice homozygous for Lpcat3^{tm1c(EUCOMM)Wtsi} and hemizygous for Tg(Alb-cre)21Mgn

• after adenovirus injection, adult mice also exhibit a decrease in ad-lib (fed) plasma TG levels, not observed in mice homozygous for Lpcat3^{tm1c(EUCOMM)Wtsi} and hemizygous for Tg(Alb-cre)21Mgn

liver/biliary system

abnormal liver physiology ( J:220827 )

• after adenovirus injection, fasted mice show a markedly reduced rate of hepatic TG secretion in response to treatment with the lipase inhibitor tyloxapol

Genotype
MGI:5822858

cn2

• Allelic Composition: Lpcat3^{tm1c(EUCOMM)Wtsi}/Lpcat3^{tm1c(EUCOMM)Wtsi}; Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
• Genetic Background: involves: C57BL/6 * C57BL/6N * SJL
• Cell Lines: EPD0455_5_A07

homeostasis/metabolism

abnormal circulating apolipoprotein level ( J:220827 )

• fractionation of plasma lipoproteins revealed lower levels of apolipoprotein B (apoB) in the VLDL fraction of chow-fed mice following an overnight fast

• however, total plasma apoB levels and protein levels of apoB in liver are similar to those in control mice

• increased levels of plasma apoB-100 following a western diet for 9 weeks

abnormal lipid homeostasis ( J:220827 )

• absence of arachidonoyl phospholipids profoundly affects lipid movement within membranes and lipidation of apoB-containing lipoproteins

abnormal lipid level ( J:220827 )

• mice secrete lipid-poor very low-density lipoprotein (VLDL) lacking arachidonoyl phospholipids

• plasma VLDL particles are markedly smaller and poorly lipidated

• EM analysis of liver samples showed reduced nascent lipoprotein particle size in the lumen of Golgi and secretory vesicles

decreased circulating triglyceride level ( J:220827 )

• decreased plasma triglyceride (TG) levels following an overnight fast on a chow diet

• lower total plasma TG levels following a western diet (40% high fat and 0.2% cholesterol) for 9 weeks

decreased circulating VLDL triglyceride level ( J:220827 )

• TG levels in the VLDL plasma fraction are markedly reduced following an overnight fast

• striking loss of TG in the VLDL plasma lipoprotein fraction following a western diet for 9 weeks

increased liver cholesterol level ( J:220827 )

• prominent accumulation of hepatic cholesterol following a western diet for 9 weeks

• increase in total cholesterol ester and a number of cholesterol ester species on a chow diet, with generally similar results on a western diet

abnormal phospholipid level ( J:220827 )

• ~70% reduction in the abundance of 16:0, 20:4 phosphatidylcholine (PC) and 18:0, 20:4 PC (two of the most abundant arachidonoyl PC species in liver membranes) on a chow diet with compensatory increases in the abundance of other PC species, esp. those containing monounsaturated chains such as 16:0, 18:1 PC and 18:0, 18:1 PC

• increased abundance of certain PC species, such as 16:0, 18:1 PC, on a western diet with similar deficits in 16:0, 20:4 PC and 18:0, 20:4 PC as observed on a chow diet

• additional reductions in 16:1, 18:2 PC and 18:1, 20:4 PC in western diet-fed livers

• severe reductions of phosphatidlyethanolamine (PE) species containing arachidonate chains on both chow and western diets

• highly selective reductions in 16:0, 20:4 PC and 18:0, 20:4 PC in plasma VLDL fractions on a chow diet, suggesting that phospholipid deficits of liver membranes are passed on to the VLDL particles that they generate

• severe loss of arachidonate in liver on a chow diet is observed in phospholipids (PLs), but not in TGs or in cholesterol esters

• total level of PC is not significantly altered in liver under either diet

decreased circulating VLDL phospholipid level ( J:220827 )

• highly selective reductions in 16:0, 20:4 PC and 18:0, 20:4 PC in plasma VLDL fractions on a chow diet, suggesting that phospholipid deficits of liver membranes are passed on to the VLDL particles that they generate

increased liver triglyceride level ( J:220827 )

• trend towards increased TG stores in the liver following an overnight fast

• prominent accumulation of hepatic TGs following a western diet for 9 weeks

• accumulation of hepatic TGs following a high-sucrose diet for 3 weeks

liver/biliary system

increased liver cholesterol level ( J:220827 )

• prominent accumulation of hepatic cholesterol following a western diet for 9 weeks

• increase in total cholesterol ester and a number of cholesterol ester species on a chow diet, with generally similar results on a western diet

increased liver triglyceride level ( J:220827 )

• trend towards increased TG stores in the liver following an overnight fast

• prominent accumulation of hepatic TGs following a western diet for 9 weeks

• accumulation of hepatic TGs following a high-sucrose diet for 3 weeks

hepatic steatosis ( J:220827 )

• histological evidence of increased lipid accumulation in the liver on a chow diet

Genotype
MGI:5822876

cn3

• Allelic Composition: Lpcat3^{tm1c(EUCOMM)Wtsi}/Lpcat3^{tm1c(EUCOMM)Wtsi}; Tg(Vil1-cre)997Gum/0
• Genetic Background: involves: C57BL/6 * C57BL/6N * SJL
• Cell Lines: EPD0455_5_A07

growth/size/body

decreased body weight ( J:220827 )

• decreased body weight at 1 week of age

• however, body weight is normal at birth

postnatal growth retardation ( J:220827 )

• pups fail to thrive and exhibit severe growth retardation

• however, suckling is normal

homeostasis/metabolism

decreased circulating glucose level ( J:220827 )

• blood glucose levels are very low at 1 week of age

decreased circulating insulin level ( J:220827 )

• plasma insulin levels are significantly lower at 1 week of age

abnormal lipid level ( J:220827 )

• massive accumulation of cytosolic lipid droplets in intestinal enterocytes at 1 week of age

decreased circulating triglyceride level ( J:220827 )

• plasma triglyceride (TG) levels are significantly lower at 1 week of age

digestive/alimentary system

abnormal enterocyte morphology ( J:220827 )

• massive accumulation of cytosolic lipid droplets in intestinal enterocytes at 1 week of age