Lpcat3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Lpcat3^{tm1c(EUCOMM)Wtsi}
• Name: lysophosphatidylcholine acyltransferase 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5822780
• Synonyms: F, Lpcat3^fl
• Gene: Lpcat3 Location: Chr6:124639887-124681142 bp, + strand Genetic Position: Chr6, 59.17 cM
• Alliance: Lpcat3^{tm1c(EUCOMM)Wtsi} page
• IMPC: Lpcat3 gene page

Mutation origin

• Mutant Cell Line: EPD0455_5_A07
• Germline Transmission: Earliest citation of germline transmission: J:220827
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 124675731 of Chromosome 6 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 124676557. Flp-mediated recombination removed the reporter and selection cassettes and left exon 3 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:220827)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lpcat3 Mutation: 25 strains or lines available

References

• Original: J:220827 Rong X, et al., Lpcat3-dependent production of arachidonoyl phospholipids is a key determinant of triglyceride secretion. Elife. 2015;4
• All: 4 reference(s)