Tg(Vil1-cre)997Gum
Transgene Detail
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Symbol: |
Tg(Vil1-cre)997Gum |
Name: |
transgene insertion 997, Deborah L Gumucio |
MGI ID: |
MGI:2448639 |
Synonyms: |
12.4KbVilCre, Tg(Vil-cre)997Gum, Vcre, VilCre, Vil-Cre, Villin-cre, Villin.cre, VillinCre |
Transgene: |
Tg(Vil1-cre)997Gum Location: Chr17:55633958-55648511 bp Genetic Position: Chr17, Syntenic
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Alliance: |
Tg(Vil1-cre)997Gum page
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Strain of Origin: |
(C57BL/6J x SJL)F1
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Transgene Type: |
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Transgenic (Recombinase) |
Mutations: |
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Insertion, Intergenic deletion
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Mutation details: A 12.4-kb promoter fragment from the villin 1 gene drives the expression of cre recombinase cDNA with the entire metallothionein sequence and a poly(A) signal. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. Seven lines were generated. According to the corresponding author (J:78815), expression is generally continuous but a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil gene expression onset of 9.0 dpc. Line 997 contains 16 copies of the transgene inserted in chromosome 17 about 119 kb upstream of St6gal2 (chr17:55,466,255; NCBI37/mm9) with a 14.6 kb deletion at the integration site.
(J:78815)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Vil1
(species not specified)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.
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Original: |
J:78815 Madison BB, et al., cis Elements of the Villin Gene Control Expression in Restricted Domains of the Vertical (Crypt) and Horizontal (Duodenum, Cecum) Axes of the Intestine. J Biol Chem. 2002 Sep 6;277(36):33275-83 |
All: |
602 reference(s) |
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