About   Help   FAQ
Transgene Detail
Symbol: Tg(Vil1-cre)997Gum
Name: transgene insertion 997, Deborah L Gumucio
MGI ID: MGI:2448639
Synonyms: 12.4KbVilCre, Tg(Vil-cre)997Gum, Vcre, VilCre, Vil-Cre, Villin-cre, Villin.cre, VillinCre
Transgene: Tg(Vil1-cre)997Gum  Location: Chr17:55633958-55648511 bp  Genetic Position: Chr17, Syntenic
Alliance: Tg(Vil1-cre)997Gum page
Strain of Origin:  (C57BL/6J x SJL)F1
Transgene Type:    Transgenic (Recombinase)
Mutations:    Insertion, Intergenic deletion
Mutation detailsA 12.4-kb promoter fragment from the villin 1 gene drives the expression of cre recombinase cDNA with the entire metallothionein sequence and a poly(A) signal. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. Seven lines were generated. According to the corresponding author (J:78815), expression is generally continuous but a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil gene expression onset of 9.0 dpc. Line 997 contains 16 copies of the transgene inserted in chromosome 17 about 119 kb upstream of St6gal2 (chr17:55,466,255; NCBI37/mm9) with a 14.6 kb deletion at the integration site. (J:78815)
 Tissue activity of this recombinase allele
Driver: Vil1 (species not specified)
Summary of all recombinase alleles driven by Vil1.

View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Mice Carrying this Mutation: 52060 assay results
Tumor Data
List all tumor models in MMHCdb carrying Tg(Vil1-cre)997Gum
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.
Original:  J:78815 Madison BB, et al., cis Elements of the Villin Gene Control Expression in Restricted Domains of the Vertical (Crypt) and Horizontal (Duodenum, Cecum) Axes of the Intestine. J Biol Chem. 2002 Sep 6;277(36):33275-83
All:  602 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Copyright, and Privacy Statement
Send questions and comments to User Support.
last database update
MGI 6.22
The Jackson Laboratory