Tg(Vil1-cre)997Gum
Transgene Detail

Summary

• Symbol: Tg(Vil1-cre)997Gum
• Name: transgene insertion 997, Deborah L Gumucio
• MGI ID: MGI:2448639
• Synonyms: 12.4KbVilCre, Tg(Vil-cre)^997Gum, V^cre, VilCre, Vil-Cre, Villin-cre, Villin.cre, VillinCre
• Transgene: Tg(Vil1-cre)997Gum Location: Chr17:55633958-55648511 bp Genetic Position: Chr17, Syntenic
• Alliance: Tg(Vil1-cre)997Gum page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A 12.4-kb promoter fragment from the villin 1 gene drives the expression of cre recombinase cDNA with the entire metallothionein sequence and a poly(A) signal. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. Seven lines were generated. According to the corresponding author (J:78815), expression is generally continuous but a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil gene expression onset of 9.0 dpc. Line 997 contains 16 copies of the transgene inserted in chromosome 17 about 119 kb upstream of St6gal2 (chr17:55,466,255; NCBI37/mm9) with a 14.6 kb deletion at the integration site. (J:78815)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Vil1 (species not specified) Summary of all recombinase alleles driven by Vil1. Comparative matrix view of recombinase activities

Disease models

Expression

In Mice Carrying this Mutation:

52060 assay results

Tumor Data

List all tumor models in MMHCdb carrying Tg(Vil1-cre)997Gum

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.

References

• Original: J:78815 Madison BB, et al., cis Elements of the Villin Gene Control Expression in Restricted Domains of the Vertical (Crypt) and Horizontal (Duodenum, Cecum) Axes of the Intestine. J Biol Chem. 2002 Sep 6;277(36):33275-83
• All: 594 reference(s)