Analysis Tools|
Allele Symbol Allele Name Allele ID |
Triotm1Mzhu targeted mutation 1, Min-Sheng Zhu MGI:4839231 |
||||||||||||
| Summary |
2 genotypes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice exhibit normal behavior
|
| N |
• mice exhibit normal cerebellum development
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Growth retardation, ataxia, small brain size, and reduced olfactory bulb size in Triotm1Mzhu/Triotm1Mzhu Tg(Nes-cre)1Kln/0 mice
|
• remaining mice die between P5 and P22
|
|
• 90% of mice die within 1 day of birth
|
|
• between P8 and P21 glial fibers become progressively sparse unlike in wild-type mice
|
|
• in granule cells
|
|
• cerebellar granule neurons exhibit impaired neurite growth compared with wild-type cells
• neurite outgrowth is unresponsive to Netrin-1 and Semaphorin 6A induction unlike wild-type cells
• however, neurite ougrowth is normally induced by glutamate and nerve growth factor
|
|
• most granules remain outside of the Purkinje cell layer and are randomly at the external granular layer and molecular layer unlike in wild-type mice
• cultured granule cells exhibit decreased migration with random orientations along their abnormal processes compared with similarly treated wild-type mice
|
|
• parallel fibers are thick and non-compact compared to in wild-type mice
• mice lack horizontal beams of parallel fibers in the inner external granule cell layer compared with wild-type mice
|
|
• the rostral migratory stream is enlarged compared to in wild-type mice
|
|
• at P21
|
|
• indistinguishable with the internal granule cell layer
|
|
• at P0.5, the inner granular layer lacks granule cells compared to in wild-type mice
|
|
• granule cells in the external granule layer exhibit disorganized cell bodies with abnormal organization of parallel fibers compared with wild-type mice
• granules exhibit short and highly branched processes, irregular growth cone with highly branched terminals, and strong filipodia in the axon shaft and the tips of leading process compared with wild-type cells
|
|
• some sub-lobules fail to form unlike in wild-type mice
• however, all lobules do form
|
|
• at P0.5, P6, and P21
|
|
• at P21
|
|
• between P8 and P21 glial fibers become progressively sparse unlike in wild-type mice
|
|
• in granule cells
|
|
• cerebellar granule neurons exhibit impaired neurite growth compared with wild-type cells
• neurite outgrowth is unresponsive to Netrin-1 and Semaphorin 6A induction unlike wild-type cells
• however, neurite ougrowth is normally induced by glutamate and nerve growth factor
|
|
• most granules remain outside of the Purkinje cell layer and are randomly at the external granular layer and molecular layer unlike in wild-type mice
• cultured granule cells exhibit decreased migration with random orientations along their abnormal processes compared with similarly treated wild-type mice
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 09/30/2025 MGI 6.24 |
|
|
|
||
