Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tardbptm1.1Pcw mutation
(1 available);
any
Tardbp mutation
(60 available)
Tg(CAG-cre/Esr1*)5Amc mutation
(9 available)
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growth/size/body
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• after cre induction by tamoxifen, mice show decrease in body weight relative to controls
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adipose tissue
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• absence of fatty acid vacuoles in adipocytes in interscapular brown fat is detected
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• reduction of fatty acid vacuoles in adipocytes in subcutaneous fat is detected
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tardbptm1.1Pcw mutation
(1 available);
any
Tardbp mutation
(60 available)
Tg(CAG-cre/Esr1*)5Amc mutation
(9 available)
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growth/size/body
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• after cre induction by tamoxifen, mice show decrease in body relative to controls
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cellular
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• increased fat oxidation after loss of Tardbp results in fat loss in adipocytes
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adipose tissue
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• inspection of animals dying after cre induction by tamoxifen shows loss of body fat in these mice
• analyses of carcasses show significant decreases in whole body fat mass, but not lean mass
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• absence of fatty acid vacuoles in adipocytes in interscapular brown fat is detected
• adipocytes are present in brown fat, but lack of stored fat within adipocytes results in loss of fat content in animals after induction by tamoxifen
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• dramatic fat loss is observed
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• reduction of fatty acid vacuoles in adipocytes in subcutaneous fat is detected
• adipocytes are present in white fat, but lack of stored fat within adipocytes results in loss of fat content in animals after induction by tamoxifen
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homeostasis/metabolism
mortality/aging
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• when cre expression is induced with a tamoxifen-containing diet (400 mg/kg of chow), mice die by day 9 post-introduction of tamoxifen
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growth/size/body
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• during the initial 3 days after cre induction by tamoxifen, all mice show decrease in body weight due to decreased food intake, but whereas controls regain recover some weight, experimental mice do not
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• mice show a cumulative loss of body weight compared to controls following cre induction, but energy intake (cumulative food intake) is similar to controls
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homeostasis/metabolism
adipose tissue
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• inspection of animals dying after cre induction by tamoxifen shows loss of body fat in these mice
• analyses of carcasses show significant decreases in whole body fat mass, but not lean mass
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cellular
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• increased fat oxidation after induction is basis for increased weight loss
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behavior/neurological
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• energy intake on day 7 is significantly higher than on day 1 after cre induction
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growth/size/body
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• during the initial 3 days after cre induction by tamoxifen, all mice show decrease in body weight due to decreased food intake, but whereas controls regain recover some weight, experimental mice do not
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homeostasis/metabolism
adipose tissue
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• inspection of animals dying after cre induction by tamoxifen shows loss of body fat in these mice
• analyses of carcasses show significant decreases in whole body fat mass, but not lean mass
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|
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tardbptm1.1Pcw mutation
(1 available);
any
Tardbp mutation
(60 available)
Tg(Stra8-icre)1Reb mutation
(2 available)
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reproductive system
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• most cauda epididymal sperm are morphologically abnormal
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• ~55% of sperm tails are curved/bent
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• roughly 50% of cauda sperm show deformed or detached heads
• ~55% of sperm show head and midpiece bent over the principal piece of the tail
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• headless sperm are observed
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• extensive male germ cell depletion is observed at P35
• germ cell depletion is progressively worse at 3, 7, and 21 months of age
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• males exhibit 7.4 times less cauda epididymal sperm than control males
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• TUNEL staining showed a significant increase in spermatocyte apoptosis
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• vacuole formation is observed within the seminiferous epithelium at P35
• at advanced ages, the seminiferous epithelium only consists of Sertoli cells
• onset of seminiferous epithelium pathology with reduced cellularity is at P12
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• at P35, the seminiferous tubule diameter appears narrower than in control males
• a 1.6-fold reduction in tubule cross-section diameter is seen at 3 months of age
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• atrophy of the seminiferous tubules, germ cell loss, and vacuole formation are observed at P35
• vacuole formation is progressively worse at 3, 7, and 21 months of age
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• testis size is severely decreased at P35
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• a 2.9-fold reduction in testis weight is seen at 3 months of age
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• diplotene-like spermatocytes display nonhomologous chromosome synapsis
• zygotene-like spermatocytes show nonhomologous chromosome synapsis, short chromosome length, and abnormal number of chromosomes
• pachytene-like spermatocytes show nonhomologous chromosome synapsis, with persistent gammaH2AX flares/foci on autosomes likely indicating unrepaired double-strand DNA breaks
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• at P35, spermatogonia and a few meiotic cells can be seen but no round spermatids are detected
• at 3-4 months of age, flow cytometry data showed a significant decrease in 1N cells (round spermatids) and an increase in 2N (spermatogonia, Sertoli, peritubular, and Leydig cells) and 4N (primary spermatocytes) cells, indicating arrest in meiosis
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• spermatocytes show failure to complete prophase I of meiosis with arrest at the mid-pachytene stage
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• 3-month-old males are severely subfertile, producing a significantly lower number of litters than control males over a 6-month period (0.6 litters versus 6 litters, respectively)
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cellular
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• most cauda epididymal sperm are morphologically abnormal
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• ~55% of sperm tails are curved/bent
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• roughly 50% of cauda sperm show deformed or detached heads
• ~55% of sperm show head and midpiece bent over the principal piece of the tail
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• headless sperm are observed
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• extensive male germ cell depletion is observed at P35
• germ cell depletion is progressively worse at 3, 7, and 21 months of age
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• males exhibit 7.4 times less cauda epididymal sperm than control males
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• zygotene-like spermatocytes show short chromosome length and abnormal number of chromosomes
• some pachytene-like spermatocytes show abnormally long chromosomes
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• zygotene-like spermatocytes show abnormal number of chromosomes likely due to abnormal synapsis between nonhomologous chromosomes
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• TUNEL staining showed a significant increase in spermatocyte apoptosis
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• pachytene-like spermatocytes with synapsis defects show gammaH2AX foci/flares on autosomes, indicating delayed DSB repair
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endocrine/exocrine glands
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• vacuole formation is observed within the seminiferous epithelium at P35
• at advanced ages, the seminiferous epithelium only consists of Sertoli cells
• onset of seminiferous epithelium pathology with reduced cellularity is at P12
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• at P35, the seminiferous tubule diameter appears narrower than in control males
• a 1.6-fold reduction in tubule cross-section diameter is seen at 3 months of age
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• atrophy of the seminiferous tubules, germ cell loss, and vacuole formation are observed at P35
• vacuole formation is progressively worse at 3, 7, and 21 months of age
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• testis size is severely decreased at P35
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• a 2.9-fold reduction in testis weight is seen at 3 months of age
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homeostasis/metabolism
Analysis Tools