All Phenotypes Drosha<tm1Litt> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Drosha^tm1Litt/Drosha^tm1.1Litt Gt(ROSA)26Sor^{tm1(cre/ERT)Nat}/Gt(ROSA)26Sor⁺	involves: 129 * 129P2/OlaHsd * C57BL/6 * FVB/N	MGI:3806251
cn2	Drosha^tm1Litt/Drosha^tm1Litt Wt1^{tm2(cre/ERT2)Wtp}/Wt1⁺	involves: 129P2/OlaHsd * 129S4/SvJae	MGI:6887989
cn3	Drosha^tm1Litt/Drosha^tm1.1Litt Tg(Cd4-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2 * FVB/N	MGI:3806244
cn4	Drosha^tm1Litt/Drosha^tm1Litt Tg(KRT5-rtTA)1Glk/0 Tg(tetO-cre)1Jaw/0	involves: 129P2/OlaHsd * C57BL/6 * FVB/N	MGI:5431584
cn5	Drosha^tm1Litt/Drosha^tm1Litt Tg(Six2-EGFP/cre)1Amc/0	involves: 129P2/OlaHsd * CD-1	MGI:6887995
cn6	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3806254
cn7	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3806253

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Gt(ROSA)26Sor^{tm1(cre/ERT)Nat}/Gt(ROSA)26Sor⁺
Genetic Background	involves: 129 * 129P2/OlaHsd * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1.1Litt mutation (0 available); any Drosha mutation (96 available)
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Gt(ROSA)26Sor^{tm1(cre/ERT)Nat} mutation (3 available); any Gt(ROSA)26Sor mutation (942 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal interferon-gamma secretion ( J:138683 )

• naive CD4+ T cells release more interferon gamma than controls after cre-mediated deletion of Rnasen induced by tamoxifen treatment

• deficient CD4+ T cells are capable of differentiating into Th1 or Th2 cells

Allelic Composition	Drosha^tm1Litt/Drosha^tm1Litt Wt1^{tm2(cre/ERT2)Wtp}/Wt1⁺
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJae

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Wt1^{tm2(cre/ERT2)Wtp} mutation (1 available); any Wt1 mutation (34 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

renal/urinary system

renal/urinary system phenotype ( J:321428 )

N	• normal kidney size with fully developed glomeruli

increased urine protein level ( J:321428 )

• protein deposits in kidney tubules by age P28

homeostasis/metabolism

increased urine protein level ( J:321428 )

• protein deposits in kidney tubules by age P28

neoplasm

neoplasm ( J:321428 )

N	• no kidney tumor development in mice up to 6 months old

mortality/aging

mortality/aging ( J:321428 )

N	• viable; live to at least 6 months

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• cachexic animals become moribund rapidly and are euthanized; by 6 months, 50% mortality in mutants is observed

growth/size/body

decreased body fat mass ( J:138683 )

• moribund animals show complete loss of visceral adipose tissue

cachexia ( J:138683 )

• displayed by many animals at 4 months

immune system

immune system phenotype ( J:138683 )

N	• spleen and lymph nodes are not larger than controls despite T cell abnormalities

increased granulocyte number ( J:138683 )

• higher frequency of granulocytes in spleen and lymph nodes is observed

increased gamma-delta T cell number ( J:138683 )

• increase is detected in spleen and lymph nodes

increased activated T cell number ( J:138683 )

• significant increase in frequency of activated (CD62L^lo44^hi) CD4+ T cells is observed in spleen and lymph nodes

decreased lymphocyte cell number ( J:138683 )

• T lymphopenia in CD8+ compartment is observed

decreased thymocyte number ( J:138683 )

• total thymocyte number is decreased compared to controls at 6 weeks; reduced frequency of TCRbeta+ thymocytes with downregulated CD14 and CD69 is observed

• absolute number of mature thymocytes is significantly reduced

decreased double-positive T cell number ( J:138683 )

• absolute number of double positive (DP) thymocytes is significantly reduced compared to controls at 6 weeks

decreased CD8-positive, alpha-beta T cell number ( J:138683 )

• significant reduction in frequency of mature CD8+ T cells is observed in periphery at 6 weeks

decreased regulatory T cell number ( J:138683 )

• mature peripheral T reg cells are reduced in frequency

abnormal immune system physiology ( J:138683 )

• very high frequencies of interferon gamma- or IL-17A-secreting cells are observed; high frequency of interferon gamma/IL-17A double secreting cells is detected in moribund animals

• slight increase in frequency of interferon gamma- or IL-17A-secreting CD4+ T cells can be seen at 3 months

• regulatory T cells have reduced suppressive activity compared to controls; in vitro proliferation of stimulated CD25-CD4+ T cells is only partially suppressed

• deficient CD4+ T cells are capable of differentiating into Th1 or Th2 cells

intestinal inflammation ( J:138683 )

• small foci of inflammatory cells are more prevalent than in controls

liver inflammation ( J:138683 )

• infiltrating cells accumulate around most blood vessels in liver with additional foci in sinusoids

lung inflammation ( J:138683 )

• infiltrating cells accumulate primarily around blood vessels, resulting in epithelial thickening of surrounding tissue

hematopoietic system

increased granulocyte number ( J:138683 )

• higher frequency of granulocytes in spleen and lymph nodes is observed

increased gamma-delta T cell number ( J:138683 )

• increase is detected in spleen and lymph nodes

increased activated T cell number ( J:138683 )

• significant increase in frequency of activated (CD62L^lo44^hi) CD4+ T cells is observed in spleen and lymph nodes

decreased lymphocyte cell number ( J:138683 )

• T lymphopenia in CD8+ compartment is observed

decreased thymocyte number ( J:138683 )

• total thymocyte number is decreased compared to controls at 6 weeks; reduced frequency of TCRbeta+ thymocytes with downregulated CD14 and CD69 is observed

• absolute number of mature thymocytes is significantly reduced

decreased double-positive T cell number ( J:138683 )

• absolute number of double positive (DP) thymocytes is significantly reduced compared to controls at 6 weeks

decreased CD8-positive, alpha-beta T cell number ( J:138683 )

• significant reduction in frequency of mature CD8+ T cells is observed in periphery at 6 weeks

decreased regulatory T cell number ( J:138683 )

• mature peripheral T reg cells are reduced in frequency

adipose tissue

decreased body fat mass ( J:138683 )

• moribund animals show complete loss of visceral adipose tissue

liver/biliary system

liver inflammation ( J:138683 )

• infiltrating cells accumulate around most blood vessels in liver with additional foci in sinusoids

respiratory system

lung inflammation ( J:138683 )

• infiltrating cells accumulate primarily around blood vessels, resulting in epithelial thickening of surrounding tissue

muscle

decreased skeletal muscle mass ( J:138683 )

• moribund animals exhibit reduction in muscle mass

digestive/alimentary system

intestinal inflammation ( J:138683 )

• small foci of inflammatory cells are more prevalent than in controls

endocrine/exocrine glands

decreased thymocyte number ( J:138683 )

• total thymocyte number is decreased compared to controls at 6 weeks; reduced frequency of TCRbeta+ thymocytes with downregulated CD14 and CD69 is observed

• absolute number of mature thymocytes is significantly reduced

Allelic Composition	Drosha^tm1Litt/Drosha^tm1Litt Tg(KRT5-rtTA)1Glk/0 Tg(tetO-cre)1Jaw/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Tg(KRT5-rtTA)1Glk mutation (0 available)
Tg(tetO-cre)1Jaw mutation (5 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

integument

integument phenotype ( J:183487 )

N	• doxycycline-treated mice retain hair follicle stem cells in early telogen and anagen

abnormal coat/ hair morphology ( J:183487 )

• hair phenotype in doxycycline-treated mice appears more slowly than in Dicer1^tm1Smr/Dicer1^tm1Smr Tg(KRT5-rtTA)1Glk Tg(tetO-cre)1Jaw mice

abnormal hair growth ( J:183487 )

• failure of hair regrowth in doxycycline-treated mice

• failure of regression at P20 in doxycycline-treated mice and remained in an abnormal growth phase

• following hair plucking, doxycycline-treated mice fail to sustain hair growth unlike control mice

alopecia ( J:183487 )

• after P14 in doxycycline-treated mice

waved hair ( J:183487 )

• external hair becomes wavy between P12 and P14 in doxycycline-treated mice

abnormal hair shaft morphology ( J:183487 )

• in doxycycline-treated mice likely due to matrix cell apoptosis

abnormal hair follicle morphology ( J:183487 )

• at P17 in doxycycline-treated mice after degradation begins

abnormal hair follicle inner root sheath morphology ( J:183487 )

• fewer differentiation cell in doxycycline-treated mice following plucking-induced anagen initiation

hair follicle degeneration ( J:183487 )

• with extrusion of abnormally keratinized cellular material in doxycycline-treated mice

small hair follicles ( J:183487 )

• in doxycycline-treated mice following plucking-induced anagen initiation

abnormal hair cycle catagen phase ( J:183487 )

• failure of catagen in doxycycline-treated mice

abnormal hair follicle regression ( J:183487 )

• failure of regression at P20 in doxycycline-treated mice

epidermal hyperplasia ( J:183487 )

• by P20, doxycycline-treated mice exhibit thickened interfollicular epidermis compared with control mice

dry skin ( J:183487 )

• mice treated with doxycycline exhibit a temporary phenotype of dry scaly skin that is resolved in an anterior-posterior direction

scaly skin ( J:183487 )

• mice treated with doxycycline exhibit a temporary phenotype of dry scaly skin that is resolved in an anterior-posterior direction

abnormal skin physiology ( J:183487 )

cellular

increased apoptosis ( J:183487 )

• doxycycline-treated mice exhibit matrix cell apoptosis with DNA damage response unlike in control mice

growth/size/body

decreased body size ( J:183487 )

• in doxycycline-treated mice

Allelic Composition	Drosha^tm1Litt/Drosha^tm1Litt Tg(Six2-EGFP/cre)1Amc/0
Genetic Background	involves: 129P2/OlaHsd * CD-1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Tg(Six2-EGFP/cre)1Amc mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

renal/urinary system

increased kidney apoptosis ( J:321428 )

• increased apoptosis of cortical and central mesenchymal and epithelial cells

• normal apoptosis of stromal cells

abnormal kidney morphology ( J:321428 )

• increase in stromal tissue in newborns

polycystic kidney ( J:321428 )

• cystic dilation of renal tubules in newborns

abnormal renal glomerulus morphology ( J:321428 )

• no mature glomeruli in E18.5 embryos and in newborns

decreased renal glomerulus number ( J:321428 )

• reduced number of glomeruli in E16.5 embryos

abnormal kidney development ( J:321428 )

• reduced number of S-shaped bodies in E13.5 embryos with further reduction at E16.5

• immature comma- and S-shaped bodies in E16.5 embryos

• no mature glomeruli in E18.5 embryos and in newborns

abnormal nephrogenic zone morphology ( J:321428 )

• thin and discontinuous cortical nephrogenic zone

renal hypoplasia ( J:321428 )

• smaller kidneys with reduced tubular structures in newborns

dilated renal tubule ( J:321428 )

• cystic dilation in newborns

absent kidney ( J:321428 )

• at least one absent kidney in 36% of E16.5 embryos, both absent in 6.5%

mortality/aging

perinatal lethality, complete penetrance ( J:321428 )

• early perinatal mortality: no offspring beyond weaning stage

cellular

increased kidney apoptosis ( J:321428 )

• increased apoptosis of cortical and central mesenchymal and epithelial cells

• normal apoptosis of stromal cells

behavior/neurological

decreased food intake ( J:321428 )

• smaller milk spot in newborns

decreased locomotor activity ( J:321428 )

• in newborns

cardiovascular system

congestive heart failure ( J:321428 )

• causing death within hours of birth

homeostasis/metabolism

cyanosis ( J:321428 )

• in newborns

pulmonary edema ( J:321428 )

• causing death within hours of birth

growth/size/body

polycystic kidney ( J:321428 )

• cystic dilation of renal tubules in newborns

respiratory system

pulmonary edema ( J:321428 )

• causing death within hours of birth

respiratory distress ( J:321428 )

• gasping in newborns

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1.1Litt mutation (0 available); any Drosha mutation (96 available)
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• females become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal regulatory T cell morphology ( J:138683 )

• decrease in Foxp3+ CD25^lo population

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

enlarged lymph nodes ( J:138683 )

• moribund mice display massive lymphadenopathy at 2.5-3 weeks of age

abnormal interferon-gamma secretion ( J:138683 )

• hyper-Ifng secretion by CD4+ and CD8+ T cells is observed

abnormal interleukin-4 secretion ( J:138683 )

• hyper-Il-4 secretion by CD4+ T cells is observed

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

hematopoietic system

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal regulatory T cell morphology ( J:138683 )

• decrease in Foxp3+ CD25^lo population

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1.1Litt mutation (0 available); any Drosha mutation (96 available)
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• males become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

hematopoietic system

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

enlarged lymph nodes ( J:138683 )

• moribund mice display massive lymphadenopathy at 2.5-3 weeks of age

abnormal interferon-gamma secretion ( J:138683 )

• hyper-Ifng secretion by CD4+ and CD8+ T cells is observed

abnormal interleukin-4 secretion ( J:138683 )

• hyper-IL-4 secretion by CD4+ T cells is observed

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/23/2024 MGI 6.23