Phenotypes associated with this allele

• Allele Symbol: Tg(T-cre)1Lwd
• Allele Name: transgene insertion 1, Mark Lewandoski
• Allele ID: MGI:3605847
• Summary: 17 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cd2bp2^tm1.1Tbh/Cd2bp2^tm1.1Tbh Tg(T-cre)1Lwd/0	B6OlaHsd.Cg-Cd2bp2^tm1.1Tbh Tg(T-cre)1Lwd	MGI:6392048
cn2	Cited2^tm1Bha/Cited2^tm2Bha Tg(T-cre)1Lwd/0	involves: 129 * C3H * C57BL/6 * SJL	MGI:3804088
cn3	Fgf8^tm1.3Mrt/Fgf8^tm1.4Mrt Tg(T-cre)1Lwd/0	involves: 129P2/OlaHsd * C3H * C57BL/6 * CD-1	MGI:3606231
cn4	Ctnnb1^tm2Kem/Ctnnb1^tm2.1Kem Tg(T-cre)1Lwd/0	involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6	MGI:5509195
cn5	Gt(ROSA)26Sor^tm1(en/Cdx1,-EGFP)Npln/Gt(ROSA)26Sor^+ Tg(T-cre)1Lwd/0	involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6	MGI:5774469
cn6	Etv4^tm1Arbr/Etv4^tm1Arbr Etv5^tm1.1Xsun/Etv5^tm1.2Xsun Tg(T-cre)1Lwd/0	involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6 * SJL	MGI:3849054
cn7	Etv4^tm1Arbr/Etv4^+ Etv5^tm1.1Xsun/Etv5^tm1.2Xsun Tg(T-cre)1Lwd/0	involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6 * SJL	MGI:3849055
cn8	Nodal^tm1.1Mku/Nodal^tm2Mku Tg(T-cre)1Lwd/0	involves: 129S1/Sv * C3H * C57BL/6	MGI:3830291
cn9	Zic3^tm2.1Jwb/Y Tg(T-cre)1Lwd/0	involves: 129S4/SvJaeSor * 129S7/SvEvBrd * C3H * C57BL/6	MGI:5470172
cn10	Rpl10a^tm1c(EUCOMM)Hmgu/Rpl10a^tm1c(EUCOMM)Hmgu Tg(T-cre)1Lwd/0	involves: 129S4/SvJaeSor * C3H * C57BL/6N	MGI:7385105
cn11	Fgfr1^tm3.1Cxd/Fgfr1^tm3.2Cxd Tg(T-cre)1Lwd/0	involves: 129S6/SvEvTac * C3H * C57BL/6	MGI:3607121
cn12	Fgfr1^tm3.2Cxd/Fgfr1^tm3.2Cxd Tg(T-cre)1Lwd/0	involves: 129S6/SvEvTac * C3H * C57BL/6	MGI:3606230
cn13	Chd7^Gt(S20-7E1)Sor/Chd7^tm1.1Dmm Tg(T-cre)1Lwd/0	involves: 129S * C3H * C57BL/6	MGI:7518601
cn14	Eomes^tm1Rob/Eomes^tm1.1Rob Tg(T-cre)1Lwd/0	involves: 129S/SvEv * C3H * C57BL/6 * CBA	MGI:3775734
cn15	Zic3^tm1.1Smwa/Y Tg(T-cre)1Lwd/0	involves: C3H * C57BL/6	MGI:5476844
cn16	Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor^tm1Fan Tg(T-cre)1Lwd/0	involves: C3H * C57BL/6 * C57BL/6J	MGI:5498473
cn17	Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor^+ Tg(T-cre)1Lwd/0	involves: C3H * C57BL/6 * C57BL/6J	MGI:5498472

Genotype
MGI:6392048

cn1

• Allelic Composition: Cd2bp2^tm1.1Tbh/Cd2bp2^tm1.1Tbh; Tg(T-cre)1Lwd/0
• Genetic Background: B6OlaHsd.Cg-Cd2bp2^tm1.1Tbh Tg(T-cre)1Lwd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:283435 )

• mice die around E10.5

embryo

abnormal vitelline vein morphology ( J:283435 )

• delayed vasculatization

embryonic growth arrest ( J:283435 )

cardiovascular system

abnormal vitelline vein morphology ( J:283435 )

• delayed vasculatization

hemorrhage ( J:283435 )

Genotype
MGI:3804088

cn2

• Allelic Composition: Cited2^tm1Bha/Cited2^tm2Bha; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129 * C3H * C57BL/6 * SJL

cardiovascular system

abnormal heart morphology ( J:137951 )

• 3 of 14 mice exhibit defective cardiac septation and 1 of 14 mice exhibit abnormal left-right patterning

double outlet right ventricle ( J:137951 )

• both the aorta and the pulmonary trunk arise from the right ventricle

right atrial isomerism ( J:137951 )

• indicated by bilateral systemic venous sinuses and a common atrium created by complete absence of the primary atrial septum

common atrium ( J:137951 )

• common atrium created by complete absence of the primary atrial septum

dextrocardia ( J:137951 )

• in few mice

ventricular septal defect ( J:137951 )

• in few mice

abnormal heart left ventricle morphology ( J:137951 )

• dextral left ventricle

respiratory system

right pulmonary isomerism ( J:137951 )

• in few mice

endocrine/exocrine glands

absent adrenal gland ( J:137951 )

• in 10 of 14 mice

growth/size/body

right atrial isomerism ( J:137951 )

• indicated by bilateral systemic venous sinuses and a common atrium created by complete absence of the primary atrial septum

right pulmonary isomerism ( J:137951 )

• in few mice

Genotype
MGI:3606231

cn3

• Allelic Composition: Fgf8^tm1.3Mrt/Fgf8^tm1.4Mrt; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129P2/OlaHsd * C3H * C57BL/6 * CD-1

mortality/aging

neonatal lethality, complete penetrance ( J:101175 )

• mutants die shortly after birth

renal/urinary system

increased kidney apoptosis ( J:101175 )

• apoptosis is increased at E14.5 most prominently in the cortical mesenchyme and at E16.5 in the primitive epithelia

absent renal glomerulus ( J:101175 )

• no glomeruli are present at any stage of kidney development

abnormal kidney development ( J:101175 )

• at E14.5 mutant kidneys show only condensation and renal vesicle formation and not tubulogenesis or glomerulogenesis that are seen in wild-type mice

• at E16.5 evidence of cap formation is mostly missing and few vesicles remain in the mutant kidneys and those remaining vesicles do not show signs of tubulogenesis

• at E18.5 the hypocellular kidney is largely devoid of nephron epithelia

• apoptosis is increased at E14.5 most prominently in the cortical mesenchyme and at E16.5 in the primitive epithelia

abnormal ureteric bud morphology ( J:101175 )

• at E18.5 the few remaining ureteric bud radii fail to bifurcate in the cortical nephrogenic region; however, somite formation, tendon progenitor populations fro E10.5 - E14.5, and limb bud induction are normal

impaired branching involved in ureteric bud morphogenesis ( J:101175 )

• at E14.5 ureteric bud branching is dramatically reduced

kidney failure ( J:101175 )

limbs/digits/tail

hindlimb oligodactyly ( J:101175 )

• most mutants lack at least one hindlimb digit; however, limb bud induction is normal

skeleton

skeleton phenotype ( J:101175 )

N • no defects are seen in skeletal development including rib formation

cellular

increased kidney apoptosis ( J:101175 )

• apoptosis is increased at E14.5 most prominently in the cortical mesenchyme and at E16.5 in the primitive epithelia

Genotype
MGI:5509195

cn4

• Allelic Composition: Ctnnb1^tm2Kem/Ctnnb1^tm2.1Kem; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6

embryo

caudal body truncation ( J:200549 )

• severely truncated at E8.5

abnormal somite development ( J:200549 )

• at E8.5, somites are disorganized

Genotype
MGI:5774469

cn5

• Allelic Composition: Gt(ROSA)26Sor^{tm1(en/Cdx1,-EGFP)Npln}/Gt(ROSA)26Sor⁺; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6

skeleton

rib fusion ( J:231654 )

• 33% of mutants show fusion of ribs in the thoracic region

abnormal vertebrae morphology ( J:231654 )

• neonates show vertebral malformations with a higher penetrance at the cervical level than at thoracic and lumber regions

decreased thoracic vertebrae number ( J:231654 )

• reduction in the number of thoracic vertebrae (12 instead of 13)

abnormal cervical vertebrae morphology ( J:231654 )

• absence of the tuberculum anterior on C6

decreased lumbar vertebrae number ( J:231654 )

• some mutants only have five lumbar vertebrae instead of six

abnormal vertebral arch morphology ( J:231654 )

• cervical regions show malformed neural arches

vertebral fusion ( J:231654 )

• neonates show vertebral fusions with a higher penetrance at the cervical level than at thoracic and lumber regions

• cervical vertebra 1 and associated anterior arch of the atlas are fused to the basioccipital bone

vertebral transformation ( J:231654 )

• neonates show the presence of a high number of homeotic transformations

• C2/C3 are anteriorly transformed

cervical vertebral transformation ( J:231654 )

• cervical regions show anterior homeotic transformations

Genotype
MGI:3849054

cn6

• Allelic Composition: Etv4^tm1Arbr/Etv4^tm1Arbr; Etv5^tm1.1Xsun/Etv5^tm1.2Xsun; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6 * SJL

mortality/aging

neonatal lethality, complete penetrance ( J:149477 )

• mice die shortly after birth due to internal organ defects

limbs/digits/tail

abnormal limb bud morphology ( J:149477 )

• limb buds exhibit reduced cell death compared to in wild-type mice

polyphalangy ( J:149477 )

• 50% of mice have a digit 1 with three phalanges unlike in wild-type mice

preaxial polydactyly ( J:149477 )

• mice exhibit preaxial polydactyly in the hindlimbs with one or two extra digits the majority of which are triphalangeal

• however, forelimbs are normal

embryo

abnormal limb bud morphology ( J:149477 )

• limb buds exhibit reduced cell death compared to in wild-type mice

skeleton

polyphalangy ( J:149477 )

• 50% of mice have a digit 1 with three phalanges unlike in wild-type mice

Genotype
MGI:3849055

cn7

• Allelic Composition: Etv4^tm1Arbr/Etv4⁺; Etv5^tm1.1Xsun/Etv5^tm1.2Xsun; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6 * SJL

limbs/digits/tail

polydactyly ( J:149477 )

• in 6 of 8 mice

Genotype
MGI:3830291

cn8

• Allelic Composition: Nodal^tm1.1Mku/Nodal^tm2Mku; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S1/Sv * C3H * C57BL/6

cardiovascular system

abnormal direction of heart looping ( J:142430 )

• the direction of looping is reversed in about half of the embryos at E8.5 - E9.5

transposition of great arteries ( J:142430 )

abnormal interventricular septum morphology ( J:142430 )

embryo

embryo phenotype ( J:142430 )

N • unlike in mice heterozygous for Nodal ^tm1Mku and Nodal ^tm1.1Mku, no midline or anterior/head morphological defects are detected

abnormal left-right axis patterning ( J:142430 )

• expression analysis indicates complete loss of asymmetric signals in the lateral plate mesoderm

growth/size/body

right pulmonary isomerism ( J:142430 )

• by E13.5

hematopoietic system

small spleen ( J:142430 )

• small or absent

absent spleen ( J:142430 )

• small or absent

immune system

small spleen ( J:142430 )

• small or absent

absent spleen ( J:142430 )

• small or absent

respiratory system

right pulmonary isomerism ( J:142430 )

• by E13.5

Genotype
MGI:5470172

cn9

• Allelic Composition: Zic3^tm2.1Jwb/Y; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S4/SvJaeSor * 129S7/SvEvBrd * C3H * C57BL/6

mortality/aging

preweaning lethality, incomplete penetrance ( J:192577 )

♂ • despite normal numbers at E9.5, fewer than expected mice at E15.5 and weaning

cardiovascular system

abnormal heart looping ( J:192577 )

♂ • in 4 of 22 mice

limbs/digits/tail

kinked tail ( J:192577 )

♂

growth/size/body

heterotaxia ( J:192577 )

♂ • laterality defects in 4 of 18 mice

Genotype
MGI:7385105

cn10

• Allelic Composition: Rpl10a^{tm1c(EUCOMM)Hmgu}/Rpl10a^{tm1c(EUCOMM)Hmgu}; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S4/SvJaeSor * C3H * C57BL/6N

embryo

embryo phenotype ( J:328993 )

N • mice exhibit normal posterior trunk

Genotype
MGI:3607121

cn11

• Allelic Composition: Fgfr1^tm3.1Cxd/Fgfr1^tm3.2Cxd; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S6/SvEvTac * C3H * C57BL/6

mortality/aging

neonatal lethality, complete penetrance ( J:101736 )

• mutants die at birth

embryo

abnormal apical ectodermal ridge morphology ( J:101736 )

• at E10.0, in the forelimb bud the apical ectodermal ridge is wider along the dorsal ventral axis and at E10.5 shorter along the anterior posterior axis

abnormal forelimb bud morphology ( J:101736 )

• at E10 the forelimb bud is shorter along the proximal distal axis, wider along the anterior posterior axis and thicker on the dorsal ventral axis and this differences are increased at E10.5

• at E11.5 individual digit condensations cannot be identified

• the number of cells in the forelimb bud is increased 65% and 31% at E10.0 and E10.5, respectively, but decreased by 26% at E11.5 and cell death is increased starting at E10.5 although still confined to the normal cell death domain

abnormal neural plate morphology ( J:101736 )

• at E9.5 the open neural plate is expanded

abnormal somite size ( J:101736 )

• somite size is irregular

limbs/digits/tail

abnormal apical ectodermal ridge morphology ( J:101736 )

• at E10.0, in the forelimb bud the apical ectodermal ridge is wider along the dorsal ventral axis and at E10.5 shorter along the anterior posterior axis

abnormal forelimb bud morphology ( J:101736 )

• at E10 the forelimb bud is shorter along the proximal distal axis, wider along the anterior posterior axis and thicker on the dorsal ventral axis and this differences are increased at E10.5

• at E11.5 individual digit condensations cannot be identified

• the number of cells in the forelimb bud is increased 65% and 31% at E10.0 and E10.5, respectively, but decreased by 26% at E11.5 and cell death is increased starting at E10.5 although still confined to the normal cell death domain

forelimb oligodactyly ( J:101736 )

• the forelimb autopod has only 3 digits 1 with 3 phalanges and 2 with 2 phalanges

abnormal forelimb stylopod morphology ( J:101736 )

• at E17.5, the stylopod is shortened by about 15%

abnormal forelimb zeugopod morphology ( J:101736 )

• at E17.5, the zeugopod is reduced by about 12% and often fused at the distal end

abnormal hindlimb morphology ( J:101736 )

• the hindlimb is more severely affected than the forelimb

nervous system

abnormal neural plate morphology ( J:101736 )

• at E9.5 the open neural plate is expanded

skeleton

abnormal axial skeleton morphology ( J:101736 )

abnormal cartilage development ( J:101736 )

• at E17.5 the initial cartilage condensations in the forelimb are reduced in number and size

Genotype
MGI:3606230

cn12

• Allelic Composition: Fgfr1^tm3.2Cxd/Fgfr1^tm3.2Cxd; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S6/SvEvTac * C3H * C57BL/6

mortality/aging

neonatal lethality, complete penetrance ( J:101736 )

• mutants die at birth

embryo

abnormal apical ectodermal ridge morphology ( J:101736 )

• at E10.0, in the forelimb bud the apical ectodermal ridge is wider along the dorsal ventral axis and at E10.5 shorter along the anterior posterior axis

abnormal forelimb bud morphology ( J:101736 )

• at E10 the forelimb bud is shorter along the proximal distal axis, wider along the anterior posterior axis and thicker on the dorsal ventral axis and this differences are increased at E10.5

• at E11.5 individual digit condensations cannot be identified

• the number of cells in the forelimb bud is increased 65% and 31% at E10.0 and E10.5, respectively, but decreased by 26% at E11.5 and cell death is increased starting at E10.5 although still confined to the normal cell death domain

abnormal neural plate morphology ( J:101736 )

• at E9.5 the open neural plate is expanded

abnormal somite size ( J:101736 )

• somite size is irregular

limbs/digits/tail

abnormal apical ectodermal ridge morphology ( J:101736 )

• at E10.0, in the forelimb bud the apical ectodermal ridge is wider along the dorsal ventral axis and at E10.5 shorter along the anterior posterior axis

abnormal forelimb bud morphology ( J:101736 )

• at E10 the forelimb bud is shorter along the proximal distal axis, wider along the anterior posterior axis and thicker on the dorsal ventral axis and this differences are increased at E10.5

• at E11.5 individual digit condensations cannot be identified

• the number of cells in the forelimb bud is increased 65% and 31% at E10.0 and E10.5, respectively, but decreased by 26% at E11.5 and cell death is increased starting at E10.5 although still confined to the normal cell death domain

forelimb oligodactyly ( J:101736 )

• the forelimb autopod has only 3 digits 1 with 3 phalanges and 2 with 2 phalanges

abnormal forelimb stylopod morphology ( J:101736 )

• at E17.5, the stylopod is shortened by about 15%

abnormal forelimb zeugopod morphology ( J:101736 )

• at E17.5, the zeugopod is reduced by about 12% and often fused at the distal end

abnormal hindlimb morphology ( J:101736 )

• the hindlimb is more severely affected than the forelimb

nervous system

abnormal neural plate morphology ( J:101736 )

• at E9.5 the open neural plate is expanded

skeleton

abnormal axial skeleton morphology ( J:101736 )

abnormal cartilage development ( J:101736 )

• at E17.5 the initial cartilage condensations in the forelimb are reduced in number and size

Genotype
MGI:7518601

cn13

• Allelic Composition: Chd7^{Gt(S20-7E1)Sor}/Chd7^tm1.1Dmm; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S * C3H * C57BL/6

hearing/vestibular/ear

hearing/vestibular/ear phenotype ( J:310063 )

N • normal vestibular and cochlear morphologies

Genotype
MGI:3775734

cn14

• Allelic Composition: Eomes^tm1Rob/Eomes^tm1.1Rob; Tg(T-cre)1Lwd/0
• Genetic Background: involves: 129S/SvEv * C3H * C57BL/6 * CBA

embryo

embryo phenotype ( J:131055 )

N • Mendelian numbers of conditionally null embryos are recovered around E6.5

Genotype
MGI:5476844

cn15

• Allelic Composition: Zic3^tm1.1Smwa/Y; Tg(T-cre)1Lwd/0
• Genetic Background: involves: C3H * C57BL/6

embryo

abnormal left-right axis patterning ( J:194989 )

♂ • at E8.5

abnormal primitive node morphology ( J:194989 )

♂ • similar to germ line null mice

cardiovascular system

abnormal heart looping ( J:194989 )

♂ • defects in looping similar to those in germ line null mice are seen at E10.5 and E13.5

Genotype
MGI:5498473

cn16

• Allelic Composition: Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor^tm1Fan; Tg(T-cre)1Lwd/0
• Genetic Background: involves: C3H * C57BL/6 * C57BL/6J

Skeletal defects in Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor^tm1Fan Tg(T-cre)1Lwd/0 mice

mortality/aging

neonatal lethality, complete penetrance ( J:197427 )

• die at birth

embryo

decreased somite size ( J:197427 )

• at E9.5

impaired somite development ( J:197427 )

• at E8.5 somite lose their compact epithelial morphology, instead cells display a scattered pattern

• poorly defined boundaries are seen along the body axis at E8.75

• lack well separated epithelia at E9.5

• at E11.5 long stretches of mesenchymal patches that fail to segregate into repeated intervals are seen

nervous system

fused dorsal root ganglion ( J:197427 )

abnormal spinal cord morphology ( J:197427 )

• axon bundles are not properly spaced

skeleton

abnormal axial skeleton morphology ( J:197427 )

• conspicuously defective along the cervical, trunk, and lumbar regions and to a lesser extent in the tail

abnormal intervertebral disk morphology ( J:197427 )

• loss of intervertebral disks

vertebral fusion ( J:197427 )

• fusions of the neural arches and vertebral bodies

limbs/digits/tail

kinked tail ( J:197427 )

• in neonates

behavior/neurological

abnormal posture ( J:197427 )

• straightened posture in neonates

Genotype
MGI:5498472

cn17

• Allelic Composition: Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor⁺; Tg(T-cre)1Lwd/0
• Genetic Background: involves: C3H * C57BL/6 * C57BL/6J

Cervical vertebrae fusions in Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor⁺ Tg(T-cre)1Lwd/0 mice

skeleton

cervical vertebral fusion ( J:197427 )

• seen in approximately 40% of adults