Nodal^tm2Mku
Targeted Allele Detail

Summary

• Symbol: Nodal^tm2Mku
• Name: nodal growth differentiation factor; targeted mutation 2, Michael R Kuehn
• MGI ID: MGI:3830289
• Synonyms: Nodal^fl2
• Gene: Nodal Location: Chr10:61253751-61261117 bp, + strand Genetic Position: Chr10, 32.21 cM
• Alliance: Nodal^tm2Mku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142430
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxp site was inserted into intron 1 and a floxed PGK-neo cassette was inserted into the 3' UTR via homologous recombination. The PGK-neo cassette was then removed by in-vitro Cre-mediated recombination. (J:142430)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nodal Mutation: 41 strains or lines available

References

• Original: J:142430 Kumar A, et al., Broad mesodermal and endodermal deletion of Nodal at postgastrulation stages results solely in left/right axial defects. Dev Dyn. 2008 Dec;237(12):3591-601
• All: 1 reference(s)