Rpl10a^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Rpl10a^{tm1c(EUCOMM)Hmgu}
• Name: ribosomal protein L10A; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7385087
• Gene: Rpl10a Location: Chr17:28547557-28550007 bp, + strand Genetic Position: Chr17, 14.65 cM
• Alliance: Rpl10a^{tm1c(EUCOMM)Hmgu} page
• IMPC: Rpl10a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:328993
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_GT1_LF2A_LacZ_BetactP_neo cassette was inserted at position 28549234 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 28550274. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:328993)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rpl10a Mutation: 50 strains or lines available

References

• Original: J:328993 Genuth NR, et al., A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production. Nat Commun. 2022 Sep 19;13(1):5491
• All: 1 reference(s)