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Allele Symbol Allele Name Allele ID |
Dicer1tm1Mmk targeted mutation 1, Matthias Merkenschlager MGI:3578093 |
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| Summary |
11 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice show rescue of the lung phenotypes seen in single conditional Dicer1 mutants, showing smaller lungs, reduced epithelial dilation, mesenchymal thickness and mesenchymal proliferation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice show rescue of the lung phenotypes seen in single conditional Dicer1 mutants, showing smaller lungs with reduced cystic dilation of epithelial ducts
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice with tumors die by 4 to 5 weeks of age
• 30% of mice die by 8 weeks of age
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• at 2 to 3 weeks of age, mice develop facial tumors
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| N |
• mice exhibit normal frequency and function of hematopoietic stem cells and progenitors
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• mice with myeloid sarcomas exhibit increased spleen weight with blast cell infiltration unlike in wild-type mice
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• in the spleen of some mice
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• hematopoietic progenitor cells exhibit increased apoptosis and proliferation compared with wild-type cells
• megakaryocyte-erythroid progenitor cells exhibit increased apoptosis compared to in wild-type mice
• wild-type mice transplanted with transgenic hematopoietic cells exhibit cytopenia and myelodysplastic features
• however, differentiated cells exhibit normal apoptosis
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• in some mice
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• micro-megakaryocytes with hypolobulated, hyperchromatic nuclei in the bone marrow
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• impaired
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• profound in some mice
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• giant platelets in the blood
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• with all subsets of leukocytes exhibiting decreased numbers
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• B cell and B cell progenitors are decreased in the bone marrow compared to in wild-type mice
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• myeloid cell frequency is increased in the bone marrow compared to in wild-type mice
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• hypersegementation
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• texture of mineralized is altered compared to in wild-type mice
• however, trabecular and cortical bone volumes are normal
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• bone marrow vascularity is increased compared to in wild-type mice
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• mildly
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• mice exhibit reduced bone marrow stromal osteogenic colony numbers relative to total colony forming numbers compared with wild-type mice
• bone marrow stromal cultured exhibit reduced alkaline phosphatase and calcified matrix deposition compared with wild-type cultures
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• at 2 to 3 weeks of age, mice develop facial tumors
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• at 2 to 3 weeks of age, mice develop myeoblastic tumors
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• mice exhibit sporadic acute myelogenous leukemia unlike wild-type mice
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• mice exhibit sporadic myeloid sarcoma with infiltration behavior unlike wild-type mice
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• bone marrow vascularity is increased compared to in wild-type mice
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• at 2 to 3 weeks of age, mice develop facial tumors
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• mice with myeloid sarcomas exhibit increased spleen weight with blast cell infiltration unlike in wild-type mice
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• mice with myeloid sarcomas exhibit increased spleen weight with blast cell infiltration unlike in wild-type mice
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• with all subsets of leukocytes exhibiting decreased numbers
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• B cell and B cell progenitors are decreased in the bone marrow compared to in wild-type mice
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• myeloid cell frequency is increased in the bone marrow compared to in wild-type mice
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• hypersegementation
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• impaired
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• texture of mineralized is altered compared to in wild-type mice
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• cortical bone volume is increased compared to in wild-type mice
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| N |
• mice exhibit normal hematopoietic morphology
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• E12.5 lungs show increased mesenchymal proliferation
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• lungs are larger at E12.5 but are similar in size to controls at E14.5 and E16.5
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• E12.5, E14.5, and E16.5 lungs exhibit reduced branching
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• E12.5 lungs exhibit expanded mesenchyme
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• E12.5 lungs exhibit dilated epithelial ducts
• E14.5 and E16.5 lungs show cystic expansion of the epithelial ducts
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• E12.5 lungs show increased mesenchymal proliferation
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• lungs are larger at E12.5 but are similar in size to controls at E14.5 and E16.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• while B cell numbers are increased relative to in Cd79atm1(cre)Reth/Cd79a+ Bcl2l11tm1.1Ast/Bcl2l11+ Dicer1tm1Mmk/Dicer1tm1Mmk mice, numbers are still lower than in wild-type mice
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• while B cell numbers are increased relative to in Cd79atm1(cre)Reth/Cd79a+ Bcl2l11tm1.1Ast/Bcl2l11+ Dicer1tm1Mmk/Dicer1tm1Mmk mice, numbers are still lower than in wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• while B cell numbers are increased relative to in Cd79atm1(cre)Reth/Cd79a+ Dicer1tm1Mmk/Dicer1tm1Mmk mice, numbers are still lower than in wild-type mice
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• while B cell numbers are increased relative to in Cd79atm1(cre)Reth/Cd79a+ Dicer1tm1Mmk/Dicer1tm1Mmk mice, numbers are still lower than in wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• hardly detected
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• in the bone marrow
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• B cell development is almost completely blocked at the pro- to pre-B cell transition
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• hardly detected
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• in the bone marrow due to a massive increase in apoptosis
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• hardly detected
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• in the bone marrow
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• B cell development is almost completely blocked at the pro- to pre-B cell transition
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• hardly detected
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• in the bone marrow due to a massive increase in apoptosis
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• usage of Dh elements and N nucleotide insertion into Igkappa is increased compared to in wild-type mice
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• usage of Dh elements and N nucleotide insertion into Igkappa is increased compared to in wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• while B cell numbers are increased relative to in Cd79atm1(cre)Reth/Cd79a+ Dicer1tm1Mmk/Dicer1tm1Mmk mice, numbers are still lower than in wild-type mice
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• while B cell numbers are increased relative to in Cd79atm1(cre)Reth/Cd79a+ Dicer1tm1Mmk/Dicer1tm1Mmk mice, numbers are still lower than in wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• unusually high percentage of thymocytes expressed TCR gamma-delta and gamma-delta cells were prevalent in the double negative compartment and decreased numbers of alpha-beta T cells
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• decreased numbers of alpha-beta T cells
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• cell numbers in thymi were reduced nearly 10-fold relative to controls, however there were normal numbers of double negative cells in thym
• increased percentage (43% versus 11% of wildtype at 6 hours and 68% versus 30% at 24 hours) of dying thymocytes when grown in vitro
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• unusually high percentage of thymocytes expressed TCR gamma-delta and gamma-delta cells were prevalent in the double negative compartment and decreased numbers of alpha-beta T cells
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• decreased numbers of alpha-beta T cells
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• cell numbers in thymi were reduced nearly 10-fold relative to controls, however there were normal numbers of double negative cells in thym
• increased percentage (43% versus 11% of wildtype at 6 hours and 68% versus 30% at 24 hours) of dying thymocytes when grown in vitro
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• cell numbers in thymi were reduced nearly 10-fold relative to controls, however there were normal numbers of double negative cells in thym
• increased percentage (43% versus 11% of wildtype at 6 hours and 68% versus 30% at 24 hours) of dying thymocytes when grown in vitro
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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