Phenotypes associated with this allele

• Allele Symbol: Tg(TcraTcrb)425Cbn
• Allele Name: transgene insertion 425, Frank Carbone
• Allele ID: MGI:3046083
• Summary: 45 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Prkd2^tm1.1Shoy/Prkd2^tm1.1Shoy Prkd3^tm1.1Shoy/Prkd3^tm1.1Shoy Tg(Lck-cre)1Jtak/0 Tg(TcraTcrb)425Cbn/0	involves: 129 * BALB/c * C57BL/6	MGI:5805811
cn2	Ctcf^tm2Gal/Ctcf^tm2Gal Tg(Lck-cre)1Cwi/0 Tg(TcraTcrb)425Cbn/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3829020
cn3	Cops5^tm1Rpar/Cops5^tm1Rpar Tg(Lck-cre)548Jxm/0 Tg(TcraTcrb)425Cbn/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL	MGI:3783541
cn4	Foxo1^tm1Flv/Foxo1^tm1Flv Rag1^tm1Mom/Rag1^tm1Mom Tg(CD2-Il7r)1Asin/? Tg(Cd4-cre)1Cwi/? Tg(TcraTcrb)425Cbn/?	involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * DBA/2	MGI:3840971
cn5	Foxo1^tm1Flv/Foxo1^tm1Flv Rag1^tm1Mom/Rag1^tm1Mom Tg(Cd4-cre)1Cwi/? Tg(TcraTcrb)425Cbn/?	involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * DBA/2	MGI:3840970
cn6	Card11^tm1.1Litt/Card11^tm1.1Litt Gt(ROSA)26Sor^tm1(cre/ERT2)Thl/Gt(ROSA)26Sor^+ Tg(TcraTcrb)425Cbn/?	involves: 129/Sv * 129P2/OlaHsd * BALB/c * C57BL/6	MGI:5307048
cn7	Ppp4c^tm1Tht/Ppp4c^tm1Tht Tg(Lck-cre)548Jxm/0 Tg(TcraTcrb)425Cbn/0	involves: BALB/c * C57BL/6 * CBA	MGI:3699364
cn8	Tg(Cd4-cre)1Cwi/? Tg(TcraTcrb)425Cbn/? Traf6^tm2Ywc/Traf6^tm2Ywc	involves: C57BL/6 * DBA/2	MGI:3773279
cx9	Cd84^tm1Pls/Cd84^tm1Pls Tg(TcraTcrb)425Cbn/?	B6.Cg-Cd84^tm1Pls Tg(TcraTcrb)425Cbn	MGI:4438779
cx10	Tespa1^tm1Smoc/Tespa1^tm1Smoc Tg(TcraTcrb)425Cbn/0	B6.Cg-Tespa1^tm1Smoc Tg(TcraTcrb)425Cbn	MGI:5432918
cx11	Tg(TcraTcrb)425Cbn/0 Trip10^tm1.1Geha/Trip10^tm1.1Geha	B6.Cg-Trip10^tm1.1Geha Tg(TcraTcrb)425Cbn	MGI:4834266
cx12	Rnf128^tm1Cdon/Rnf128^tm1Cdon Tg(TcraTcrb)425Cbn/0	involves: 129 * BALB/c * C57BL/6	MGI:4459535
cx13	Ndfip1^Gt(RRD002)Byg/Ndfip1^Gt(RRD002)Byg Rag1^tm1Mom/Rag1^tm1Mom Tg(TcraTcrb)425Cbn/0	involves: 129P2/OlaHsd * 129S7/SvEvBrd * BALB/c * C57BL/6	MGI:5550271
cx14	Gpr15^tm1.1Litt/Gpr15^tm1.1Litt Rag2^tm1Fwa/Rag2^tm1Fwa Tg(TcraTcrb)425Cbn/0	involves: 129P2/OlaHsd * 129S/SvEv * BALB/c * C57BL/6	MGI:5518933
cx15	Tg(TcraTcrb)425Cbn/0 Trim30a^tm1Yjk/Trim30a^tm1Yjk	involves: 129P2/OlaHsd * BALB/c * C57BL/6	MGI:5606171
cx16	Tg(Ins2-TFRC/OVA)296Wehi/0 Tg(TcraTcrb)425Cbn/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3803202
cx17	Aire^tm1Mand/Aire^+ Tg(Ins2-TFRC/OVA)296Wehi/0 Tg(TcraTcrb)425Cbn/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3803203
cx18	Aire^tm1.1Doi/Aire^tm1.1Doi Tg(Ins2-TFRC/OVA)296Wehi/0 Tg(TcraTcrb)425Cbn/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3803204
cx19	Git2^Gt(XG510)Byg/Git2^Gt(XG510)Byg Tg(TcraTcrb)425Cbn/0	involves: 129P2/OlaHsd * C57BL/6	MGI:4461270
cx20	Mcl1^tm1Yen/Mcl1^tm1Yen Tg(TcraTcrb)425Cbn/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3837392
cx21	Icos^tm1Flv/Icos^tm1Flv Tg(TcraTcrb)425Cbn/0	involves: 129S1/Sv * C57BL/6	MGI:3851526
cx22	Tagln2^tm1Cdj/Tagln2^tm1Cdj Tg(TcraTcrb)425Cbn/0	involves: 129S4/SvJae * C57BL/6	MGI:6119492
cx23	Rnf128^tm1.1Flv/Y Tg(TcraTcrb)425Cbn/0	involves: 129S6/SvEvTac * BALB/c * C57BL/6	MGI:4365572
cx24	Rnf128^tm1.1Flv/Rnf128^tm1.1Flv Tg(TcraTcrb)425Cbn/0	involves: 129S6/SvEvTac * BALB/c * C57BL/6	MGI:4365571
cx25	Sh2d1a^tm1Pls/Sh2d1a^tm1Pls Tg(TcraTcrb)425Cbn/?	involves: 129S6/SvEvTac * C57BL/6	MGI:4438774
cx26	H2-T23^tm3Cant/H2-T23^tm3Cant Tg(TcraTcrb)425Cbn/?	involves: 129S6/SvEvTac * C57BL/6	MGI:3822038
cx27	Ptprc^ltng/Ptprc^tm1Mak Tg(TcraTcrb)425Cbn/0	involves: 129/Sv * BALB/c * C57BL/6	MGI:4442182
cx28	Ptprc^tm1Mak/Ptprc^tm1Mak Tg(TcraTcrb)425Cbn/0	involves: 129/Sv * BALB/c * C57BL/6	MGI:4442181
cx29	Rhoh^tm1Brak/Rhoh^tm1Brak Tg(TcraTcrb)425Cbn/?	involves: 129/Sv * C57BL/6	MGI:3826463
cx30	Elk4^tm1Rtr/Elk4^tm1Rtr Tg(TcraTcrb)425Cbn/?	involves: 129/Sv * C57BL/6	MGI:3036848
cx31	Plxna1^tm1Kik/Plxna1^tm1Kik Tg(TcraTcrb)425Cbn/?	involves: BALB/c * C57BL/6	MGI:4462168
cx32	Tg(CD2-Ceacam1*4L)1Rsb/0 Tg(TcraTcrb)425Cbn/0	involves: BALB/c * C57BL/6	MGI:3695218
cx33	Pip5k1c^tm1.2Ref/Pip5k1c^tm1.2Ref Tg(TcraTcrb)425Cbn/0	involves: BALB/c * C57BL/6	MGI:4838029
cx34	Ptpn22^tm1.1Kas/Ptpn22^tm1.1Kas Tg(TcraTcrb)425Cbn/0	involves: BALB/c * C57BL/6	MGI:5294247
cx35	Aire^tm1Mmat/Aire^tm2Mmat Tg(TcraTcrb)425Cbn/?	involves: BALB/c * C57BL/6 * CBA	MGI:3818937
cx36	Bcl6^tm1.1Toka/Bcl6^+ Tg(TcraTcrb)425Cbn/0	involves: BALB/c * C57BL/6 * NZB	MGI:5140808
cx37	Tg(CD2-Mir181d)38Oers/0 Tg(TcraTcrb)425Cbn/0	involves: C3H * C57BL/6	MGI:5588420
cx38	Lck^tm1Dbs/Lck^tm1Dbs Tg(TcraTcrb)425Cbn/0	involves: C3H * C57BL/6	MGI:3698308
cx39	Btla^tm1Dgra/Btla^tm1Dgra Tg(TcraTcrb)425Cbn/?	involves: C57BL/6	MGI:3842632
cx40	Patz1^tm1.1Welm/Patz1^tm1.1Welm Tg(TcraTcrb)425Cbn/0	involves: C57BL/6	MGI:4454319
cx41	Rasgrp1^tm1.1Wz/Rasgrp1^tm1.1Wz Tg(TcraTcrb)425Cbn/?	involves: C57BL/6 * BALB/c	MGI:5695369
cx42	Foxp3^tm1.1Ayr/Y Rag1^tm1Mom/Rag1^tm1Mom Tg(TcraTcrb)425Cbn/0	Not Specified	MGI:3574972
tg43	Tg(TcraTcrb)425Cbn/0	involves: BALB/c * C57BL/6	MGI:3760860
tg44	Tg(TcraTcrb)425Cbn/0	involves: C57BL/6	MGI:3851528
tg45	Tg(TcraTcrb)425Cbn/?	involves: BALB/c * C57BL/6	MGI:3762632

Genotype
MGI:5805811

cn1

• Allelic Composition: Prkd2^tm1.1Shoy/Prkd2^tm1.1Shoy; Prkd3^tm1.1Shoy/Prkd3^tm1.1Shoy; Tg(Lck-cre)1Jtak/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129 * BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased thymocyte number ( J:236298 )

• CD4+ or CD8+ single positive thymocytes

• reduced CD4+CD8^int intermediate cells

endocrine/exocrine glands

decreased thymocyte number ( J:236298 )

• CD4+ or CD8+ single positive thymocytes

• reduced CD4+CD8^int intermediate cells

hematopoietic system

decreased thymocyte number ( J:236298 )

• CD4+ or CD8+ single positive thymocytes

• reduced CD4+CD8^int intermediate cells

Genotype
MGI:3829020

cn2

• Allelic Composition: Ctcf^tm2Gal/Ctcf^tm2Gal; Tg(Lck-cre)1Cwi/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal T cell differentiation ( J:143271 )

• T cell development is more severely arrested than in Ctcf^tm1Laat/Ctcf^tm1Laat Tg(Lck-cre)1Cwi mice

increased double-negative T cell number ( J:143271 )

decreased double-positive T cell number ( J:143271 )

decreased T cell number ( J:143271 )

• mice exhibit an almost complete lack of mature T cells in the spleen

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

increased single-positive T cell number ( J:143271 )

• the number of immature single positive cells in the thymus is increased compared to in wild-type mice

hematopoietic system

abnormal T cell differentiation ( J:143271 )

• T cell development is more severely arrested than in Ctcf^tm1Laat/Ctcf^tm1Laat Tg(Lck-cre)1Cwi mice

increased double-negative T cell number ( J:143271 )

decreased T cell number ( J:143271 )

• mice exhibit an almost complete lack of mature T cells in the spleen

decreased double-positive T cell number ( J:143271 )

decreased CD4-positive, alpha-beta T cell number ( J:143271 )

increased single-positive T cell number ( J:143271 )

• the number of immature single positive cells in the thymus is increased compared to in wild-type mice

Genotype
MGI:3783541

cn3

• Allelic Composition: Cops5^tm1Rpar/Cops5^tm1Rpar; Tg(Lck-cre)548Jxm/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL

hematopoietic system

decreased thymocyte number ( J:132114 )

• an 80-90% reduction compared to control

immune system

decreased thymocyte number ( J:132114 )

• an 80-90% reduction compared to control

endocrine/exocrine glands

decreased thymocyte number ( J:132114 )

• an 80-90% reduction compared to control

Genotype
MGI:3840971

cn4

• Allelic Composition: Foxo1^tm1Flv/Foxo1^tm1Flv; Rag1^tm1Mom/Rag1^tm1Mom; Tg(CD2-Il7r)1Asin/?; Tg(Cd4-cre)1Cwi/?; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * DBA/2

immune system

decreased T cell number ( J:147057 )

• the Il7r transgene rescues the low T cell numbers observed in mice with Foxo1-deficient OT-II T cells

hematopoietic system

decreased T cell number ( J:147057 )

• the Il7r transgene rescues the low T cell numbers observed in mice with Foxo1-deficient OT-II T cells

Genotype
MGI:3840970

cn5

• Allelic Composition: Foxo1^tm1Flv/Foxo1^tm1Flv; Rag1^tm1Mom/Rag1^tm1Mom; Tg(Cd4-cre)1Cwi/?; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * DBA/2

immune system

decreased T cell number ( J:147057 )

• T cell numbers in the spleen and lymph nodes are reduced 80-90% compared to Rag-null OT-II controls

hematopoietic system

decreased T cell number ( J:147057 )

• T cell numbers in the spleen and lymph nodes are reduced 80-90% compared to Rag-null OT-II controls

Genotype
MGI:5307048

cn6

• Allelic Composition: Card11^tm1.1Litt/Card11^tm1.1Litt; Gt(ROSA)26Sor^{tm1(cre/ERT2)Thl}/Gt(ROSA)26Sor⁺; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: 129/Sv * 129P2/OlaHsd * BALB/c * C57BL/6

immune system

abnormal T cell activation ( J:180410 )

• reactivation is prevented after in vitro activation with Ova and tamoxifen treatment

hematopoietic system

abnormal T cell activation ( J:180410 )

• reactivation is prevented after in vitro activation with Ova and tamoxifen treatment

Genotype
MGI:3699364

cn7

• Allelic Composition: Ppp4c^tm1Tht/Ppp4c^tm1Tht; Tg(Lck-cre)548Jxm/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: BALB/c * C57BL/6 * CBA

hematopoietic system

abnormal positive T cell selection ( J:117684 )

• only marginal increase in numbers of CD4 positive thymocytes is observed compared to TCR-transgenic mice

• CD4 SP/DP ratio is 0.29 compared to 0.46 in controls, indicating reduced positive selection

decreased double-positive T cell number ( J:117684 )

• a significant decrease in unfractionated, double-positive thymocyte numbers compared to single transgenic littermates abnormal CD4 T cell morphology

decreased single-positive T cell number ( J:117684 )

• significant decrease in SP cell numbers

immune system

abnormal positive T cell selection ( J:117684 )

• only marginal increase in numbers of CD4 positive thymocytes is observed compared to TCR-transgenic mice

• CD4 SP/DP ratio is 0.29 compared to 0.46 in controls, indicating reduced positive selection

decreased double-positive T cell number ( J:117684 )

• a significant decrease in unfractionated, double-positive thymocyte numbers compared to single transgenic littermates abnormal CD4 T cell morphology

decreased single-positive T cell number ( J:117684 )

• significant decrease in SP cell numbers

Genotype
MGI:3773279

cn8

• Allelic Composition: Tg(Cd4-cre)1Cwi/?; Tg(TcraTcrb)425Cbn/?; Traf6^tm2Ywc/Traf6^tm2Ywc
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

increased interleukin-2 secretion ( J:130882 )

• CD4 T cells from mice immunized with OVA antigen secrete almost 10-fold more IL-2 upon in vitro antigen restimulate compared to mice transgenic only for the T cell receptor

abnormal lymphocyte anergy ( J:130882 )

• OVA antigen specific T cells continue to proliferate upon antigen restimulation in a transfer model of tolerance

Genotype
MGI:4438779

cx9

• Allelic Composition: Cd84^tm1Pls/Cd84^tm1Pls; Tg(TcraTcrb)425Cbn/?
• Genetic Background: B6.Cg-Cd84^tm1Pls Tg(TcraTcrb)425Cbn

immune system

abnormal CD4-positive, alpha-beta T cell physiology ( J:157915 )

• when B cells are used as the antigen presenting cells, T cells are significantly less efficient in forming conjugates as compared to their wild-type counterparts

hematopoietic system

abnormal CD4-positive, alpha-beta T cell physiology ( J:157915 )

• when B cells are used as the antigen presenting cells, T cells are significantly less efficient in forming conjugates as compared to their wild-type counterparts

Genotype
MGI:5432918

cx10

• Allelic Composition: Tespa1^tm1Smoc/Tespa1^tm1Smoc; Tg(TcraTcrb)425Cbn/0
• Genetic Background: B6.Cg-Tespa1^tm1Smoc Tg(TcraTcrb)425Cbn

immune system

abnormal T cell differentiation ( J:186446 )

• very few cells proceed to the CD4+ single positive stage

decreased CD4-positive, alpha-beta T cell number ( J:186446 )

• in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:186446 )

• in the periphery

hematopoietic system

abnormal T cell differentiation ( J:186446 )

• very few cells proceed to the CD4+ single positive stage

decreased CD4-positive, alpha-beta T cell number ( J:186446 )

• in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:186446 )

• in the periphery

Genotype
MGI:4834266

cx11

• Allelic Composition: Tg(TcraTcrb)425Cbn/0; Trip10^tm1.1Geha/Trip10^tm1.1Geha
• Genetic Background: B6.Cg-Trip10^tm1.1Geha Tg(TcraTcrb)425Cbn

immune system

immune system phenotype ( J:164363 )

N • oxazalone-treated mice exhibit normal antigen-specific T effector cell generation

abnormal effector T cell morphology ( J:164363 )

• following ovalbumin skin-challenge, effector T cells exhibit a 10-fold reduction in accumulation compared to when wild-type cells are transferred

decreased CD4-positive, alpha-beta T cell number ( J:164363 )

• accumulation of CD4+ T cells in ovalbumin-challenged ears of wild-type recipient mice is impaired compared to when wild-type cells are used

hematopoietic system

abnormal effector T cell morphology ( J:164363 )

• following ovalbumin skin-challenge, effector T cells exhibit a 10-fold reduction in accumulation compared to when wild-type cells are transferred

decreased CD4-positive, alpha-beta T cell number ( J:164363 )

• accumulation of CD4+ T cells in ovalbumin-challenged ears of wild-type recipient mice is impaired compared to when wild-type cells are used

Genotype
MGI:4459535

cx12

• Allelic Composition: Rnf128^tm1Cdon/Rnf128^tm1Cdon; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129 * BALB/c * C57BL/6

hematopoietic system

increased splenocyte proliferation ( J:160681 )

♀ • V alpha 2, CD44+ CD4+ T cells from ovalbumin primed mice when stimulated in culture fail to exhibit a reduced immune response

immune system

increased splenocyte proliferation ( J:160681 )

♀ • V alpha 2, CD44+ CD4+ T cells from ovalbumin primed mice when stimulated in culture fail to exhibit a reduced immune response

increased interferon-gamma secretion ( J:160681 )

♀ • V alpha 2, CD44+ CD4+ T cells from ovalbumin primed mice when stimulated in culture fail to exhibit a reduced immune response

increased interleukin-2 secretion ( J:160681 )

♀ • V alpha 2, CD44+ CD4+ T cells from ovalbumin primed mice when stimulated in culture fail to exhibit a reduced immune response

abnormal immune tolerance ( J:160681 )

♀ • abnormal antigen-specific CD4+ T cell tolerance

cellular

increased splenocyte proliferation ( J:160681 )

♀ • V alpha 2, CD44+ CD4+ T cells from ovalbumin primed mice when stimulated in culture fail to exhibit a reduced immune response

Genotype
MGI:5550271

cx13

• Allelic Composition: Ndfip1^{Gt(RRD002)Byg}/Ndfip1^{Gt(RRD002)Byg}; Rag1^tm1Mom/Rag1^tm1Mom; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * BALB/c * C57BL/6

immune system

immune system phenotype ( J:205692 )

N • unlike Ndfip1^{Gt(RRD002)Byg} homozygotes, mice do not develop eosinophilic inflammation and T cells do not acquire an activated phenotype in the absence of antigen

Genotype
MGI:5518933

cx14

• Allelic Composition: Gpr15^tm1.1Litt/Gpr15^tm1.1Litt; Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * BALB/c * C57BL/6

immune system

abnormal immune system physiology ( J:199126 )

• mice fed chicken ovalbumin exhibit a reduced increase the number and frequency of regulatory T cells in the large intestine lamina propria comparted with control mice

Genotype
MGI:5606171

cx15

• Allelic Composition: Tg(TcraTcrb)425Cbn/0; Trim30a^tm1Yjk/Trim30a^tm1Yjk
• Genetic Background: involves: 129P2/OlaHsd * BALB/c * C57BL/6

immune system

increased T cell proliferation ( J:215171 )

• T cells exhibit 10-20% enhanced proliferation compared to single Tg(TcraTcrb)425Cbn T cells, particularly when stimulated with a low dose of OVA(323-339) peptide

hematopoietic system

increased T cell proliferation ( J:215171 )

• T cells exhibit 10-20% enhanced proliferation compared to single Tg(TcraTcrb)425Cbn T cells, particularly when stimulated with a low dose of OVA(323-339) peptide

cellular

increased T cell proliferation ( J:215171 )

• T cells exhibit 10-20% enhanced proliferation compared to single Tg(TcraTcrb)425Cbn T cells, particularly when stimulated with a low dose of OVA(323-339) peptide

Genotype
MGI:3803202

cx16

• Allelic Composition: Tg(Ins2-TFRC/OVA)296Wehi/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

increased double-positive T cell number ( J:135154 )

• the percentage of double-positive T cells in the thymus is increased by 20 % compared to control mice

decreased CD4-positive, alpha-beta T cell number ( J:135154 )

• there is a 10 fold drop in the number of CD4 single positive thymocytes in the thymus compared to controls due to increased negative selection of T cell bearing the transgenic TCR

• the number of CD4 T cells in the periphery that express the gene segments associated with the transgenic TCR is reduced almost by half compared to mice carrying just the Tg(TcraTcrb)425Cbn OT-II transgene

hematopoietic system

increased double-positive T cell number ( J:135154 )

• the percentage of double-positive T cells in the thymus is increased by 20 % compared to control mice

decreased CD4-positive, alpha-beta T cell number ( J:135154 )

• there is a 10 fold drop in the number of CD4 single positive thymocytes in the thymus compared to controls due to increased negative selection of T cell bearing the transgenic TCR

• the number of CD4 T cells in the periphery that express the gene segments associated with the transgenic TCR is reduced almost by half compared to mice carrying just the Tg(TcraTcrb)425Cbn OT-II transgene

Genotype
MGI:3803203

cx17

• Allelic Composition: Aire^tm1Mand/Aire⁺; Tg(Ins2-TFRC/OVA)296Wehi/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal T cell differentiation ( J:135154 )

• negative selection fails to occur of CD 4 T cells bearing the transgenic TCR as evidenced by the normal number of clonotypic T cells found in the thymus and spleen

hematopoietic system

abnormal T cell differentiation ( J:135154 )

• negative selection fails to occur of CD 4 T cells bearing the transgenic TCR as evidenced by the normal number of clonotypic T cells found in the thymus and spleen

Genotype
MGI:3803204

cx18

• Allelic Composition: Aire^tm1.1Doi/Aire^tm1.1Doi; Tg(Ins2-TFRC/OVA)296Wehi/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal T cell differentiation ( J:135154 )

• negative selection of CD 4 T cells bearing the transgenic TCR is impaired as evidenced by only a slight reduction of clonotypic T cells occuring in the thymus and spleen

decreased CD4-positive, alpha-beta T cell number ( J:135154 )

• there is a slight reduction in the number of CD4 single positive thymocytes in the thymus compared to controls

hematopoietic system

abnormal T cell differentiation ( J:135154 )

• negative selection of CD 4 T cells bearing the transgenic TCR is impaired as evidenced by only a slight reduction of clonotypic T cells occuring in the thymus and spleen

decreased CD4-positive, alpha-beta T cell number ( J:135154 )

• there is a slight reduction in the number of CD4 single positive thymocytes in the thymus compared to controls

Genotype
MGI:4461270

cx19

• Allelic Composition: Git2^Gt(XG510)Byg/Git2^Gt(XG510)Byg; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:160613 )

• single positive cell number in thymus

• transgene positive CD4 cells in spleen, lymph node and blood

immune system

decreased CD4-positive, alpha-beta T cell number ( J:160613 )

• single positive cell number in thymus

• transgene positive CD4 cells in spleen, lymph node and blood

Genotype
MGI:3837392

cx20

• Allelic Composition: Mcl1^tm1Yen/Mcl1^tm1Yen; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

immune system

abnormal double-positive T cell morphology ( J:146246 )

• mice have decreased numbers of double positive thymocytes expressing the transgenic TCR

decreased CD4-positive, alpha-beta T cell number ( J:146246 )

• mice have decreased frequency and numbers of transgenic CD4⁺ T cells in the thymus and in the periphery

hematopoietic system

abnormal double-positive T cell morphology ( J:146246 )

• mice have decreased numbers of double positive thymocytes expressing the transgenic TCR

decreased CD4-positive, alpha-beta T cell number ( J:146246 )

• mice have decreased frequency and numbers of transgenic CD4⁺ T cells in the thymus and in the periphery

Genotype
MGI:3851526

cx21

• Allelic Composition: Icos^tm1Flv/Icos^tm1Flv; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129S1/Sv * C57BL/6

immune system

abnormal cytokine secretion ( J:112600 )

• naive CD4 T cells cultured with OVA peptide-loaded wild-type splenic antigen presenting cells (APCs) and restimulated with anti-CD3 and secrete less IL-4 and IL-17a and more interferon gamma (Ifng) than stimulated wild-type CD4 T cells

• CD4 T cells cultured with OVA peptide-loaded spleen cells from B7 (CD80/86) deficient mice then restimulated with anti-CD3 do not produce IL-4, IL-17a or Ifng

Genotype
MGI:6119492

cx22

• Allelic Composition: Tagln2^tm1Cdj/Tagln2^tm1Cdj; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6

immune system

abnormal T cell activation ( J:256656 )

• purified CD4+ and CD8+ T cells respectively, showed a significant reduction in IFNG and IL-2 protein levels and decreased proliferation in response to stimulation

hematopoietic system

abnormal T cell activation ( J:256656 )

• purified CD4+ and CD8+ T cells respectively, showed a significant reduction in IFNG and IL-2 protein levels and decreased proliferation in response to stimulation

Genotype
MGI:4365572

cx23

• Allelic Composition: Rnf128^tm1.1Flv/Y; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129S6/SvEvTac * BALB/c * C57BL/6

immune system

decreased T cell proliferation ( J:153216 )

♂

increased circulating interleukin-2 level ( J:153216 )

♂

abnormal peripheral T cell anergy ( J:153216 )

♂ • despite increasing antigen doses, CD4+ T cells cannot be tolerized unlike similarly treated wild-type cells and continue to exhibit increased T cell proliferation and IL2 production

homeostasis/metabolism

increased circulating interleukin-2 level ( J:153216 )

♂

hematopoietic system

decreased T cell proliferation ( J:153216 )

♂

cellular

decreased T cell proliferation ( J:153216 )

♂

Genotype
MGI:4365571

cx24

• Allelic Composition: Rnf128^tm1.1Flv/Rnf128^tm1.1Flv; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129S6/SvEvTac * BALB/c * C57BL/6

immune system

decreased T cell proliferation ( J:153216 )

♀

increased circulating interleukin-2 level ( J:153216 )

♀

abnormal peripheral T cell anergy ( J:153216 )

♀ • despite increasing antigen doses, CD4+ T cells cannot be tolerized unlike similarly treated wild-type cells and continue to exhibit increased T cell proliferation and IL2 production

homeostasis/metabolism

increased circulating interleukin-2 level ( J:153216 )

♀

hematopoietic system

decreased T cell proliferation ( J:153216 )

♀

cellular

decreased T cell proliferation ( J:153216 )

♀

Genotype
MGI:4438774

cx25

• Allelic Composition: Sh2d1a^tm1Pls/Sh2d1a^tm1Pls; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

immune system

abnormal CD4-positive, alpha-beta T cell physiology ( J:157915 )

♀ • T cell to B cell antigen specific, dose dependent conjugate formation is maintained for 10 min but not for 30 min

hematopoietic system

abnormal CD4-positive, alpha-beta T cell physiology ( J:157915 )

♀ • T cell to B cell antigen specific, dose dependent conjugate formation is maintained for 10 min but not for 30 min

Genotype
MGI:3822038

cx26

• Allelic Composition: H2-T23^tm3Cant/H2-T23^tm3Cant; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

immune system

abnormal CD4-positive, alpha-beta T cell physiology ( J:142087 )

• cells are resistant to CD8 T regulatory cells when co-transferred into an immunodeficient host

hematopoietic system

abnormal CD4-positive, alpha-beta T cell physiology ( J:142087 )

• cells are resistant to CD8 T regulatory cells when co-transferred into an immunodeficient host

Genotype
MGI:4442182

cx27

• Allelic Composition: Ptprc^ltng/Ptprc^tm1Mak; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129/Sv * BALB/c * C57BL/6

immune system

thymus hypoplasia ( J:158876 )

decreased CD4-positive, alpha-beta T cell number ( J:158876 )

• fewer Tg(TcraTcrb)425Cbn CD4+ T cells develop compared to in wild-type Tg(TcraTcrb)425Cbn mice

hematopoietic system

thymus hypoplasia ( J:158876 )

decreased CD4-positive, alpha-beta T cell number ( J:158876 )

• fewer Tg(TcraTcrb)425Cbn CD4+ T cells develop compared to in wild-type Tg(TcraTcrb)425Cbn mice

endocrine/exocrine glands

thymus hypoplasia ( J:158876 )

Genotype
MGI:4442181

cx28

• Allelic Composition: Ptprc^tm1Mak/Ptprc^tm1Mak; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129/Sv * BALB/c * C57BL/6

immune system

decreased CD4-positive, alpha-beta T cell number ( J:158876 )

• nearly absent

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:158876 )

• nearly absent

Genotype
MGI:3826463

cx29

• Allelic Composition: Rhoh^tm1Brak/Rhoh^tm1Brak; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: 129/Sv * C57BL/6

immune system

immune system phenotype ( J:143161 )

N • no increase in DN4 cells observed

decreased CD4-positive, alpha-beta T cell number ( J:143161 )

• severely impaired generation of CD4+ cells

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:143161 )

• severely impaired generation of CD4+ cells

Genotype
MGI:3036848

cx30

• Allelic Composition: Elk4^tm1Rtr/Elk4^tm1Rtr; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: 129/Sv * C57BL/6

hematopoietic system

abnormal positive T cell selection ( J:88515 )

• CD4 thymocyte positive selection is impaired; mutants generate about 75% fewer CD4⁺ single-positive thymocytes than wild-type

decreased CD4-positive, alpha-beta T cell number ( J:88515 )

• mutants generate about 75% fewer CD4⁺ single-positive thymocytes than wild-type

abnormal splenic cell ratio ( J:88515 )

• decrease in splenic CD4:CD8 ratio

immune system

abnormal positive T cell selection ( J:88515 )

• CD4 thymocyte positive selection is impaired; mutants generate about 75% fewer CD4⁺ single-positive thymocytes than wild-type

decreased CD4-positive, alpha-beta T cell number ( J:88515 )

• mutants generate about 75% fewer CD4⁺ single-positive thymocytes than wild-type

abnormal splenic cell ratio ( J:88515 )

• decrease in splenic CD4:CD8 ratio

Genotype
MGI:4462168

cx31

• Allelic Composition: Plxna1^tm1Kik/Plxna1^tm1Kik; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: BALB/c * C57BL/6

immune system

decreased T cell proliferation ( J:161856 )

• ova induced proliferative response is much reduced

hematopoietic system

decreased T cell proliferation ( J:161856 )

• ova induced proliferative response is much reduced

cellular

decreased T cell proliferation ( J:161856 )

• ova induced proliferative response is much reduced

Genotype
MGI:3695218

cx32

• Allelic Composition: Tg(CD2-Ceacam1*4L)1Rsb/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: BALB/c * C57BL/6

immune system

abnormal CD4-positive, alpha-beta T cell physiology ( J:116136 )

• CD4+ spleen cells exhibit decreased proliferation and IL-2 production in response to OVA (ovalbumin) and OVA peptide compared to Tg(TcraTcrb)425Cbn mice

hematopoietic system

abnormal CD4-positive, alpha-beta T cell physiology ( J:116136 )

• CD4+ spleen cells exhibit decreased proliferation and IL-2 production in response to OVA (ovalbumin) and OVA peptide compared to Tg(TcraTcrb)425Cbn mice

Genotype
MGI:4838029

cx33

• Allelic Composition: Pip5k1c^tm1.2Ref/Pip5k1c^tm1.2Ref; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: BALB/c * C57BL/6

immune system

decreased dendritic cell number ( J:164732 )

decreased B cell number ( J:164732 )

abnormal T cell physiology ( J:164732 )

• adhesion to ICAM-1 is increased compared to in wild-type cells

• T cell-APC conjugate formation is decreased compared to in wild-type cells

increased T cell proliferation ( J:164732 )

• CD4+ T cells exhibit increased proliferation in vitro and in vivo compared with similarly treated wild-type cells

increased interferon-gamma secretion ( J:164732 )

• following activation with ovalbumin peptide and restimulation with anti-CD3 and anti-CD28 antibodies

increased interleukin-2 secretion ( J:164732 )

• following activation with ovalbumin peptide and restimulation with anti-CD3 and anti-CD28 antibodies

hematopoietic system

decreased dendritic cell number ( J:164732 )

decreased B cell number ( J:164732 )

abnormal T cell physiology ( J:164732 )

• adhesion to ICAM-1 is increased compared to in wild-type cells

• T cell-APC conjugate formation is decreased compared to in wild-type cells

increased T cell proliferation ( J:164732 )

• CD4+ T cells exhibit increased proliferation in vitro and in vivo compared with similarly treated wild-type cells

cellular

increased T cell proliferation ( J:164732 )

• CD4+ T cells exhibit increased proliferation in vitro and in vivo compared with similarly treated wild-type cells

Genotype
MGI:5294247

cx34

• Allelic Composition: Ptpn22^tm1.1Kas/Ptpn22^tm1.1Kas; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: BALB/c * C57BL/6

immune system

increased T cell proliferation ( J:176519 )

• in naive spleen and lymph node in response to TCR stimulation and enhanced and prolonged Lck and ZAP-70 tyrosine phosphorylation

increased CD4-positive, alpha-beta T cell number ( J:176519 )

• mice exhibit increased positive selection of thymic and peripheral T cells compared with wild-type mice

hematopoietic system

increased T cell proliferation ( J:176519 )

• in naive spleen and lymph node in response to TCR stimulation and enhanced and prolonged Lck and ZAP-70 tyrosine phosphorylation

increased CD4-positive, alpha-beta T cell number ( J:176519 )

• mice exhibit increased positive selection of thymic and peripheral T cells compared with wild-type mice

cellular

increased T cell proliferation ( J:176519 )

• in naive spleen and lymph node in response to TCR stimulation and enhanced and prolonged Lck and ZAP-70 tyrosine phosphorylation

Genotype
MGI:3818937

cx35

• Allelic Composition: Aire^tm1Mmat/Aire^tm2Mmat; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: BALB/c * C57BL/6 * CBA

immune system

immune system phenotype ( J:141379 )

N • autoimmune pathology is absent unlike in mice not carrying Tg(TcraTcrb)425Cbn

abnormal thymus morphology ( J:141379 )

• GFP expressing cells are less complex in shape compared to GFP expressing cells in mice with one wild type allele

• distribution of GFP expressing cells is altered similar to mice homozygous for Aire^tm2Mmat

hematopoietic system

abnormal thymus morphology ( J:141379 )

• GFP expressing cells are less complex in shape compared to GFP expressing cells in mice with one wild type allele

• distribution of GFP expressing cells is altered similar to mice homozygous for Aire^tm2Mmat

endocrine/exocrine glands

abnormal thymus morphology ( J:141379 )

• GFP expressing cells are less complex in shape compared to GFP expressing cells in mice with one wild type allele

• distribution of GFP expressing cells is altered similar to mice homozygous for Aire^tm2Mmat

Genotype
MGI:5140808

cx36

• Allelic Composition: Bcl6^tm1.1Toka/Bcl6⁺; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: BALB/c * C57BL/6 * NZB

immune system

decreased T cell proliferation ( J:174014 )

• T follicular helper cells in mice immunized with NP-ovalbumin exhibit accelerated proliferatively quiescent unlike wild-type cells

hematopoietic system

decreased T cell proliferation ( J:174014 )

• T follicular helper cells in mice immunized with NP-ovalbumin exhibit accelerated proliferatively quiescent unlike wild-type cells

cellular

decreased T cell proliferation ( J:174014 )

• T follicular helper cells in mice immunized with NP-ovalbumin exhibit accelerated proliferatively quiescent unlike wild-type cells

Genotype
MGI:5588420

cx37

• Allelic Composition: Tg(CD2-Mir181d)38Oers/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: C3H * C57BL/6

hematopoietic system

decreased T cell number ( J:212571 )

• dramatic reduction in the periphery

immune system

decreased T cell number ( J:212571 )

• dramatic reduction in the periphery

Genotype
MGI:3698308

cx38

• Allelic Composition: Lck^tm1Dbs/Lck^tm1Dbs; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: C3H * C57BL/6

hematopoietic system

abnormal positive T cell selection ( J:114660 )

• positive selection of CD4 and CD8 lineage is compromised

arrested T cell differentiation ( J:114660 )

• almost complete block of positive selection is observed

immune system

abnormal positive T cell selection ( J:114660 )

• positive selection of CD4 and CD8 lineage is compromised

arrested T cell differentiation ( J:114660 )

• almost complete block of positive selection is observed

Genotype
MGI:3842632

cx39

• Allelic Composition: Btla^tm1Dgra/Btla^tm1Dgra; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: C57BL/6

immune system

abnormal peripheral T cell anergy ( J:147751 )

• T cells are resistant to tolerance induced by peptide injections

• T cells from mutant mice pre-treated with OVA produce higher levels of IL-2 and IFN-gamma, and proliferate more vigorously in response to OVA restimulation than do controls

• IFN-gamma production is higher for T cells from mutant mice pre-treated with OVA compared to mutant mice pre-treated with PBS

Genotype
MGI:4454319

cx40

• Allelic Composition: Patz1^tm1.1Welm/Patz1^tm1.1Welm; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: C57BL/6

hematopoietic system

abnormal T cell subpopulation ratio ( J:158947 )

• slightly higher percentage of CD4+ T cells and correspondingly lower frequency of CD8+ T

increased CD4-positive, alpha-beta T cell number ( J:158947 )

decreased CD8-positive, alpha-beta T cell number ( J:158947 )

immune system

abnormal T cell subpopulation ratio ( J:158947 )

• slightly higher percentage of CD4+ T cells and correspondingly lower frequency of CD8+ T

increased CD4-positive, alpha-beta T cell number ( J:158947 )

decreased CD8-positive, alpha-beta T cell number ( J:158947 )

Genotype
MGI:5695369

cx41

• Allelic Composition: Rasgrp1^tm1.1Wz/Rasgrp1^tm1.1Wz; Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: C57BL/6 * BALB/c

immune system

abnormal CD4-positive T cell differentiation ( J:225109 )

• only 2% CD4+ thymocytes compared to 26% in heterozygous controls

hematopoietic system

abnormal CD4-positive T cell differentiation ( J:225109 )

• only 2% CD4+ thymocytes compared to 26% in heterozygous controls

Genotype
MGI:3574972

cx42

• Allelic Composition: Foxp3^tm1.1Ayr/Y; Rag1^tm1Mom/Rag1^tm1Mom; Tg(TcraTcrb)425Cbn/0
• Genetic Background: Not Specified

immune system

immune system phenotype ( J:97029 )

♂N • mice exhibit no differences in thymocyte and peripheral T cell subpopulations and normal antigen sensitivity, costimulation requirement and proliferative capacity of nonregulatory CD4+ T cells

normal phenotype

no abnormal phenotype detected ( J:97029 )

♂ • mice were healthy and phenotypically indistinguishable from controls

Genotype
MGI:3760860

tg43

• Allelic Composition: Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: BALB/c * C57BL/6

hematopoietic system

abnormal positive T cell selection ( J:125548 )

• positive selection of CD4+ T cells is significantly greater in the thymus compared to Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr mice

immune system

abnormal positive T cell selection ( J:125548 )

• positive selection of CD4+ T cells is significantly greater in the thymus compared to Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr mice

Genotype
MGI:3851528

tg44

• Allelic Composition: Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: C57BL/6

immune system

abnormal cytokine level ( J:112600 )

• CD4 T cells cultured with OVA peptide-loaded spleen cells from B7 (CD80/86) deficient mice then restimulated with anti-CD3 produce lower amounts of IL-4, Il-17a, and interferon gamma (Ifng) than wild-type T cells

homeostasis/metabolism

abnormal cytokine level ( J:112600 )

• CD4 T cells cultured with OVA peptide-loaded spleen cells from B7 (CD80/86) deficient mice then restimulated with anti-CD3 produce lower amounts of IL-4, Il-17a, and interferon gamma (Ifng) than wild-type T cells

Genotype
MGI:3762632

tg45

• Allelic Composition: Tg(TcraTcrb)425Cbn/?
• Genetic Background: involves: BALB/c * C57BL/6

immune system

abnormal positive T cell selection ( J:87876 )

• positive selection of CD4+ T cells is significantly greater in the thymus and lymph nodes compared to non-transgenic contro

increased CD4-positive, alpha-beta T cell number ( J:87876 )

• cells from thymus and lymph nodes exhibit a fourfold increase in CD4 to CD8 peripheral T cell ratios as compared to non-transgenic control

hematopoietic system

abnormal positive T cell selection ( J:87876 )

• positive selection of CD4+ T cells is significantly greater in the thymus and lymph nodes compared to non-transgenic contro

increased CD4-positive, alpha-beta T cell number ( J:87876 )

• cells from thymus and lymph nodes exhibit a fourfold increase in CD4 to CD8 peripheral T cell ratios as compared to non-transgenic control