Pip5k1c^tm1.2Ref
Targeted Allele Detail

Summary

• Symbol: Pip5k1c^tm1.2Ref
• Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma; targeted mutation 1.2, Reinhard Fassler
• MGI ID: MGI:4838023
• Synonyms: PIPKIgamma90^-, PIPKIgammadeltaE17
• Gene: Pip5k1c Location: Chr10:81128797-81155807 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Pip5k1c^tm1.2Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164732
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 17. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 17. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exon 17. The absence of the long protein isoform expression was confirmed by western blot analysis on T cell extracts. (J:180016)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pip5k1c Mutation: 42 strains or lines available

References

• Original: J:164732 Wernimont SA, et al., PIPKI gamma 90 negatively regulates LFA-1-mediated adhesion and activation in antigen-induced CD4+ T cells. J Immunol. 2010 Oct 15;185(8):4714-23
• All: 4 reference(s)