Phenotypes associated with this allele
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
|
|
|
normal phenotype
|
|
• no apparent phenotype unless loxP flanked regions are deleted via Cre recombinase activity
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm2Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Tg(KRT5-cre)5132Jlj mutation
(1 available)
|
|
|
integument
|
|
• majority of auchene hair type are altered in shape, many with more pronounced bends
|
|
|
• adult coat appears less dense and ruffled than controls
|
|
|
• about 90% of zigzag (ZZ) type hair show much less pronounced bends in 6-month old adults
• hair forms exhibit a variable number of bends compared to the three bend in control hairs; many do not have alternating bend patterns of control hairs, but have 2 bends in same direction
|
|
|
• ~90% of zigzag (ZZ) type hair shows much less pronounced bends in 6-month old adults
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Tg(KRT5-cre)5132Jlj mutation
(1 available)
|
|
|
integument
|
|
• majority of auchene hair type are altered in shape, many with more pronounced bends
|
|
|
• adult coat appears less dense and ruffled than controls
|
|
|
• about 90% of zigzag (ZZ) type hair show much less pronounced bends in 6-month old adults
• hair forms exhibit a variable number of bends compared to the three bend in control hairs; many do not have alternating bend patterns of control hairs, but have 2 bends in same direction
|
|
|
• ~90% of zigzag (ZZ) type hair shows much less pronounced bends in 6-month old adults
|
nervous system
|
|
• reduction of sensory innervtion of the epidermic at P0
|
|
|
• reduction in epidermal nerve fiber density
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Etv6tm1(RUNX1)Haho mutation
(1 available);
any
Etv6 mutation
(142 available)
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Tg(Mx1-cre)1Cgn mutation
(7 available)
|
|
|
mortality/aging
|
|
• all mice die within 8 days of treatment with pIpC unlike control mice
|
hematopoietic system
|
|
• severe following pIpC treatment
|
|
|
• following pIpC treatment
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Etv6tm1(RUNX1)Haho mutation
(1 available);
any
Etv6 mutation
(142 available)
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Tg(Mx1-cre)1Cgn mutation
(7 available)
|
|
|
hematopoietic system
|
|
• following treatment with pIpC, the number of progenitors, enriched hematopoietic stem cells, and pure hematopoietic stem cells are increased compared to similarly treated wild-type mice
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Tg(Cdh5-cre)1Spe mutation
(2 available)
|
|
|
mortality/aging
|
|
• 65% of embryos die between E12.5 and E15.5
|
hematopoietic system
|
|
• 10% of E12.5 embryos have a pale liver due to anemia
|
|
|
• hematopoietic stem cell first derive as clusters of Kithigh cells from the endothelium of dorsal aorta and vitelline and umbilical arteries of E10.5 conceptus
• these clusters are absent in mutant E10.5 conceptus and there is a 90-fold decrease in the Kithigh cells
|
|
|
• there is about a 8-fold decrease in CFUs from the yolk sac, vitelline and umbilical arteries, placenta and fetal liver
• over 99% of the CFUs that do develop in culture have one functional Runx1 allele
• decreased hematopoietic stem cell numbers are reflected in poor engraftment of mutant bone marrow cells into irradiated recipients
• CFUs cultured from bone marrow of transplant recipients are much reduced compared to controls and over 99% also have one functional Runx1 allele
|
cardiovascular system
|
|
• 10% of E12.5 embryos have hemorrhaging within the central nervous system
|
liver/biliary system
|
|
• 10% of E12.5 embryos have a pale liver due to anemia
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Slc17a8tm1(cre)Lowl mutation
(0 available);
any
Slc17a8 mutation
(40 available)
|
|
|
behavior/neurological
|
N |
• thermal nociception, chemical nociception, and neuropathic mechanical pain are not markedly affected in mutants
• mechanical allodynia is unaffected in mutants
|
|
|
• intraplantar injection of carrageenan still induces mechanical hypersensitivity in mutants, but a modest (significant) increase in mechanical thresholds compared to controls is observed (higher magnitude of mechanical stimulus required to evoke paw withdrawal)
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Tg(VAV1-cre)1Graf mutation
(1 available)
|
|
|
hematopoietic system
|
|
• increased numbers of CFU-G and CFU-GEMM can be derived from E15.5 fetal livers compared to controls
|
|
|
• numbers are increased almost 3-fold
|
|
|
• increased numbers of CFU-G and CFU-GEMM can be derived from E15.5 fetal livers compared to controls
|
|
|
• lymphocyte numbers are reduced by about a third
|
|
|
• there is about a 10-fold reduction in thymocyte number
|
|
|
• numbers in the thymus are decreased by half
|
|
|
• B cell numbers are greatly reduced
|
|
|
• numbers are increased over 2-fold
|
|
|
• lin- Sca-1+ kit+ cell numbers in the bone marrow are increased about 5 fold
• increased numbers of CFU-C can be derived from E15.5 fetal livers compared to controls
• the vast majority of the CFUs have both alleles of the Runx1 gene deleted
|
immune system
|
|
• numbers are increased almost 3-fold
|
|
|
• lymphocyte numbers are reduced by about a third
|
|
|
• there is about a 10-fold reduction in thymocyte number
|
|
|
• numbers in the thymus are decreased by half
|
|
|
• B cell numbers are greatly reduced
|
|
|
• numbers are increased over 2-fold
|
endocrine/exocrine glands
|
|
• there is about a 10-fold reduction in thymocyte number
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Tg(Mx1-cre)1Cgn mutation
(7 available)
U2af1tm1.1Hev mutation
(1 available);
any
U2af1 mutation
(33 available)
|
|
|
hematopoietic system
|
|
• poly (IC) treated mice develop multilineage cytopenia
|
|
|
• mice develop low-level myeloid dysplasia after poly (IC) treatment
|
|
|
• mice develop thrombocytopenia after poly (IC) treatment
|
|
|
• mice exhibit an increased percentage of myeloid cells and increased myeloid colony formation after poly (IC) treatment
|
|
|
• mice develop low-level erythroid dysplasia after poly (IC) treatment
|
homeostasis/metabolism
mortality/aging
|
N |
• poly (IC) treated mice have a normal life span and do not develop frank leukemia within 1.5 years after poly (IC) treatment
|
|
|
• mice treated with ENU after poly (IC) treatment die prematurely with myeloid pathology
|
neoplasm
|
|
• one mouse treated with ENU after poly (IC) treatment developed acute myeloid leukemia
• mice treated with ENU after poly (IC) treatment exhibit increased percentages of myeloid cells in the peripheral blood, enlarged spleen, and extramedullary hematopoiesis, occasional low-grade dysplasia in the erythroid and neutrophil lineages indicating the development of myeloproliferative neoplasm
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Slc17a8tm1(cre)Lowl mutation
(0 available);
any
Slc17a8 mutation
(40 available)
|
|
|
nervous system
|
|
• most sensory neurons innervating hair follicles of the hairy skin display tdTomato +ve unmyelinated circumferential endings in contrast to the longitudinal unmyelinated lanceolate endings observed in control skin; these circumferential endings are located ventral to tdTomato -ve, NF200 +ve myelinated (non-sensory) circumferential endings
• around hairs in the epidermis no decrease in tdTomato +ve free nerve endings is observed; morphology of dermal papillae-epidermis nerve endings in thick glabrous skin is unchanged
• innervation of the touch dome by tdTomato labeled myelinated mechanoreceptors is not changed
|
|
|
• mutants show a marked loss of mechanosensitive neurons compared to controls, with a concurrent increase in the number of nonsensitive neurons
|
|
|
• average current density in remaining mechanosensitive small neurons is reduced compared to control mice, but no change in the average current density is detected in medium/large tdTomato-labeled neurons
|
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Spi1tm2.1Dgt mutation
(0 available);
any
Spi1 mutation
(27 available)
Tg(Mx1-cre)1Cgn mutation
(7 available)
|
|
|
immune system
|
|
• after pIpC treatment, thymus weight is less than in wild-type mice but greater than in Runx1tm3Spe/Runx1tm3Spe Tg(Mx1-cre)1Cgn mice
|
|
|
• after pIpC treatment, the number of Gr-1+Mac1+ cells in the spleen is increased 8-fold compared to in wild-type mice
|
|
|
• after pIpC treatment, the B cell compartment is reduced in the spleen and bone marrow
|
hematopoietic system
|
|
• after pIpC treatment, thymus weight is less than in wild-type mice but greater than in Runx1tm3Spe/Runx1tm3Spe Tg(Mx1-cre)1Cgn mice
|
|
|
• after pIpC treatment, the number of Gr-1+Mac1+ cells in the spleen is increased 8-fold compared to in wild-type mice
|
|
|
• after pIpC treatment, the B cell compartment is reduced in the spleen and bone marrow
|
endocrine/exocrine glands
|
|
• after pIpC treatment, thymus weight is less than in wild-type mice but greater than in Runx1tm3Spe/Runx1tm3Spe Tg(Mx1-cre)1Cgn mice
|
growth/size/body
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
Tg(Mx1-cre)1Cgn mutation
(7 available)
|
|
|
hematopoietic system
|
|
• after pIpC treatment, the number of DN3 and DN4 T cells is decreased 3-fold compared to in wild-type mice
|
|
|
• after pIpC treatment, the number of DN1 increased 3-fold compared to in wild-type mice
|
|
|
• after pIpC treatment, T cell maturation is blocked at the DN2 to DN3 stage
|
|
|
• following pIpC treatment, myeloid progenitors are increased compared to in untreated mice
|
|
|
• two weeks after pIpC treatment
|
|
|
• following pIpC treatment, bone marrow B cells are lost unlike in untreated controls
|
|
|
• following pIpC treatment, the number of long-term repopulating hematopoietic stem cells is decreased compared to in untreated controls
|
|
|
• following pIpC treatment, the hematopoietic stem cells- enriched LKS+ population is increased unlike in untreated controls
|
homeostasis/metabolism
immune system
|
|
• after pIpC treatment, the number of DN3 and DN4 T cells is decreased 3-fold compared to in wild-type mice
|
|
|
• after pIpC treatment, the number of DN1 increased 3-fold compared to in wild-type mice
|
|
|
• after pIpC treatment, T cell maturation is blocked at the DN2 to DN3 stage
|
|
|
• following pIpC treatment, bone marrow B cells are lost unlike in untreated controls
|
mortality/aging
|
|
• all mice die prematurely after receiving ENU, within 15 months after poly (IC) treatment
|
neoplasm
|
|
• all mice die prematurely after receiving ENU, within 15 months after poly (IC) treatment, with myeloid pathology including increased percentages of myeloid cells in the peripheral blood, enlarged spleen, and extramedullary hematopoiesis, occasional low-grade dysplasia in the erythroid and neutrophil lineages indicating the development of myeloproliferative neoplasm
|
endocrine/exocrine glands
growth/size/body
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapttm2Arbr mutation
(1 available);
any
Mapt mutation
(428 available)
Runx1tm1(cre/Esr1*)Ims mutation
(0 available);
any
Runx1 mutation
(35 available)
Runx1tm3Spe mutation
(0 available);
any
Runx1 mutation
(35 available)
|
|
|
nervous system
|
|
• few or no tyrosine hydroxylase C-low threshold mechanoreceptors (C-LTMRs) are observed in P21 mice following tamoxifen administration at P2
• decrease in the number of longitudinal lanceolate endings (characteristic of C-LTMRs) in back hairy skin of P21 mice
|
Analysis Tools
