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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Rag2tm1.1Cgn
targeted mutation 1.1, University of Cologne
MGI:2654909
Summary 6 genotypes


Genotype
MGI:2654915
hm1
Allelic
Composition		 Rag2tm1.1Cgn/Rag2tm1.1Cgn
Genetic
Background		 C57BL/6-Rag2tm1.1Cgn
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2tm1.1Cgn mutation (4 available); any Rag2 mutation (117 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
arrested B cell differentiation ( J:72214 )
• B cell development is arrested at the pro-B cell stage
arrested T cell differentiation ( J:72214 )
• T cell development is arrested at the pro-T cell stage

immune system
arrested B cell differentiation ( J:72214 )
• B cell development is arrested at the pro-B cell stage
arrested T cell differentiation ( J:72214 )
• T cell development is arrested at the pro-T cell stage




Genotype
MGI:5447537
cn2
Allelic
Composition		 Nr3c1tm1.1Jda/Nr3c1tm1.1Jda
Rag2tm1.1Cgn/Rag2tm1.1Cgn
Tg(Lck-cre)548Jxm/0
Tg(TcrAND)53Hed/0
Genetic
Background		 B6.Cg-Rag2tm1.1Cgn Nr3c1tm1.1Jda Tg(Lck-cre)548Jxm Tg(TcrAND)53Hed
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nr3c1tm1.1Jda mutation (1 available); any Nr3c1 mutation (34 available)
Rag2tm1.1Cgn mutation (4 available); any Rag2 mutation (117 available)
Tg(Lck-cre)548Jxm mutation (2 available)
Tg(TcrAND)53Hed mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system

hematopoietic system




Genotype
MGI:2654913
cn3
Allelic
Composition		 Rag2tm1Cgn/Rag2tm1.1Cgn
Tg(Mx1-cre)1Cgn/0
Genetic
Background		 involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2tm1.1Cgn mutation (4 available); any Rag2 mutation (117 available)
Rag2tm1Cgn mutation (2 available); any Rag2 mutation (117 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
absent immature B cells ( J:72214 )
• the IgM+HSAhigh immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old
arrested B cell differentiation ( J:72214 )
• B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
arrested T cell differentiation ( J:72214 )
• T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
decreased mature B cell number ( J:72214 )
• the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days
• the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction
• mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year
• mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction
• mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression
• when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal
decreased follicular B cell number ( J:72214 )
• the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days
decreased marginal zone B cell number ( J:72214 )
• marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth
• the number of marginal zone B cells increases slightly with age but remains well below normal levels
• marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age
increased plasma cell number ( J:72214 )
• the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls
absent pre-B cells ( J:72214 )
• pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old
abnormal B cell activation ( J:72214 )
• peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth
• the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age
• B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age

hematopoietic system
absent immature B cells ( J:72214 )
• the IgM+HSAhigh immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old
arrested B cell differentiation ( J:72214 )
• B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
arrested T cell differentiation ( J:72214 )
• T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old
decreased mature B cell number ( J:72214 )
• the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days
• the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction
• mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year
• mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction
• mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression
• when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal
decreased follicular B cell number ( J:72214 )
• the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days
decreased marginal zone B cell number ( J:72214 )
• marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth
• the number of marginal zone B cells increases slightly with age but remains well below normal levels
• marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age
increased plasma cell number ( J:72214 )
• the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls
absent pre-B cells ( J:72214 )
• pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old
abnormal B cell activation ( J:72214 )
• peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth
• the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age
• B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age




Genotype
MGI:5317331
cx4
Allelic
Composition		 Rag2tm1.1Cgn/Rag2tm1.1Cgn
Trex1tm1Tld/Trex1tm1Tld
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2tm1.1Cgn mutation (4 available); any Rag2 mutation (117 available)
Trex1tm1Tld mutation (2 available); any Trex1 mutation (22 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
mortality/aging ( J:181257 )
N
• autoimmune pathology and mortality observed in Trex1tm1Tld homozygotes are rescued regardless of hematopoietic reconstitution with bone marrow from Ifnar1tm1Agt homozygotes

immune system
immune system phenotype ( J:181257 )
N
• autoimmune pathology and mortality observed in Trex1tm1Tld homozygotes are rescued regardless of hematopoietic reconstitution with bone marrow from Ifnar1tm1Agt homozygotes




Genotype
MGI:5429705
cx5
Allelic
Composition		 Rag2tm1.1Cgn/Rag2tm1.1Cgn
Tg(HLA-DR2)#Lfug/0
Tg(TCROb.1A12)#Lfug/0
Genetic
Background		 involves: C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag2tm1.1Cgn mutation (4 available); any Rag2 mutation (117 available)
Tg(HLA-DR2)#Lfug mutation (0 available)
Tg(TCROb.1A12)#Lfug mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
autoimmune response ( J:134764 )
• clinical manifestations included both classical and non-classical EAE symptoms
CNS inflammation ( J:134764 )
• focal inflammatory lesions are seen throughout the central nervous system
• inflammatory infiltrate with variable cellular composition, usually containing neutrophilic granulocytes, activated macrophages and T lymphocytes

behavior/neurological
hindlimb paralysis ( J:134764 )
• seen in all mice with an age of onset ranging from 48 to 102 days

nervous system
CNS inflammation ( J:134764 )
• focal inflammatory lesions are seen throughout the central nervous system
• inflammatory infiltrate with variable cellular composition, usually containing neutrophilic granulocytes, activated macrophages and T lymphocytes
demyelination ( J:134764 )
• focal demyelinated tissue lesions are seen throughout the central nervous system, including the optic and vestibular nerves

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
multiple sclerosis DOID:2377 OMIM:612594
OMIM:612595
OMIM:612596
 J:134764




Genotype
MGI:5906764
cx6
Allelic
Composition		 Dusp5tm1.1Achn/Dusp5tm1.1Achn
Rag2tm1.1Cgn/Rag2tm1.1Cgn
Genetic
Background		 involves: C57BL/6NCrl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dusp5tm1.1Achn mutation (0 available); any Dusp5 mutation (16 available)
Rag2tm1.1Cgn mutation (4 available); any Rag2 mutation (117 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased eosinophil cell number ( J:218203 )
• circulating at 6 and 13 following N. brasiliensis infection
• in the blood, bronchoalveolar lavage fluid, spleen and bone marrow at 14 days following N. brasiliensis infection
abnormal eosinophil physiology ( J:218203 )
• following N. brasiliensis infection, eosiniphils exhibit a cell intrinsic 2- to 3-fold increased proliferation and decreased apoptosis compared with cells from control mice
abnormal susceptibility to parasitic infection ( J:218203 )
• following N. brasiliensis infection, mice exhibit increased circulating eosinophils at 6 and 13 days compared with control mice
• following N. brasiliensis infection, mice exhibit an increased in eosinophils in the blood, bronchoalveolar lavage fluid, spleen and bone marrow at 14 days compared with control mice
• following N. brasiliensis infection, mice exhibit lower worm burden compared with control mice
• treatment with antibodies to deplete NK cells does not alter phenotype

hematopoietic system
increased eosinophil cell number ( J:218203 )
• circulating at 6 and 13 following N. brasiliensis infection
• in the blood, bronchoalveolar lavage fluid, spleen and bone marrow at 14 days following N. brasiliensis infection
abnormal eosinophil physiology ( J:218203 )
• following N. brasiliensis infection, eosiniphils exhibit a cell intrinsic 2- to 3-fold increased proliferation and decreased apoptosis compared with cells from control mice




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory