Phenotypes associated with this allele

• Allele Symbol: Rest⁺
• Allele Name: wild type
• Allele ID: MGI:2433490
• Summary: 10 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Rest^tm2b(EUCOMM)Wtsi/Rest^+	C57BL/6N-Rest^tm2b(EUCOMM)Wtsi/Ieg	MGI:5757614
ht2	Rest^tm1.2Bban/Rest^+	involves: 129S4/SvJaeSor * FVB/N	MGI:6378687
cn3	Rest^tm1.1Yasu/Rest^+ H2az2^Tg(Wnt1-cre)11Rth/H2az2^+	involves: 129S4/SvJae * C57BL/6 * C57BL/6J * CBA/J	MGI:6241489
cn4	Rest^tm1.1Yasu/Rest^+ H2az2^Tg(Wnt1-cre)11Rth/H2az2^+ Tg(Dct-lacZ)A12Jkn/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:6241536
cn5	Rest^tm1.1Yasu/Rest^+ Tg(KRT14-cre)1Cgn/0	involves: 129S4/SvJae * C57BL/6 * DBA/2	MGI:6241529
cn6	Rest^tm1.1Yasu/Rest^+ Tg(Tyr-cre)1Lru/Y	involves: 129S4/SvJae * C57BL/6 * DBA/2	MGI:6241517
cn7	Rest^tm1.1Yasu/Rest^+ Tg(Mpz-cre)26Mes/0	involves: 129S4/SvJae * C57BL/6 * FVB/NTac	MGI:6241492
cn8	Rest^tm1.1Bban/Rest^+ Gfi1^tm1(cre)Gan/Gfi1^+	involves: 129S4/SvJaeSor * 129S7/SvEvBrd * FVB/N	MGI:6378689
cn9	Rest^tm1.1Bban/Rest^+ Gt(ROSA)26Sor^tm1(cre/ERT2)Tyj/Gt(ROSA)26Sor^+	involves: 129S4/SvJaeSor * C57BL/6J	MGI:6378690
cn10	Dct^tm1(cre)Bee/Dct^+ Rest^tm1.1Yasu/Rest^+	involves: 129/Sv * 129S4/SvJae * C57BL/6	MGI:6241494

Genotype
MGI:5757614

ht1

• Allelic Composition: Rest^{tm2b(EUCOMM)Wtsi}/Rest⁺
• Genetic Background: C57BL/6N-Rest^{tm2b(EUCOMM)Wtsi}/Ieg
• Cell Lines: EPD0156_1_D10

Data Sources

IMPC Data for Rest

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hearing/vestibular/ear

abnormal auditory brainstem response ( J:211773 )

IMPC - HMGU

hematopoietic system

decreased mean platelet volume ( J:211773 )

IMPC - HMGU

Genotype
MGI:6378687

ht2

• Allelic Composition: Rest^tm1.2Bban/Rest⁺
• Genetic Background: involves: 129S4/SvJaeSor * FVB/N

hearing/vestibular/ear

abnormal kinocilium morphology ( J:265659 )

• kinocilia are abnormally short in the utricles at E15 and E16

abnormal outer hair cell stereociliary bundle morphology ( J:265659 )

• most outer hair cells lack stereocilia at P6

• organ of Corti cultures from P1 mice show degeneration of outer hair cell stereocilia

• organ of Corti cultures from P1 mice treated with the HDAC inhibitors FK228, SAHA, or Merck60 show prevention of outer hair cell stereocilia degeneration

• mice injected with the HDAC inhibitor SAHA at P2, P4, and P5 show retention of stereocilia in outer hair cells

• SAHA treatment is more effective at rescuing the outer hair cells at P6 than at P16

cochlear hair cell degeneration ( J:265659 )

• hair cells are present until E16-17 but degenerate throughout the inner ear by P12

• the hearing organ of P6 mice shows increased apoptosis in hair cells

• the caspase inhibitors Z-DEVD-FMK and Z-VAD-FMK delay the death of hair cells in organ of Corti cultures but do not prevent the degeneration of stereocilia

cochlear inner hair cell degeneration ( J:265659 )

abnormal utricle morphology ( J:265659 )

• cilia are abnormally short in the utricles at E15 and E16

increased or absent threshold for auditory brainstem response ( J:265659 )

deafness ( J:265659 )

behavior/neurological

impaired balance ( J:265659 )

• mice exhibit balance defects

cellular

abnormal kinocilium morphology ( J:265659 )

• kinocilia are abnormally short in the utricles at E15 and E16

nervous system

nervous system phenotype ( J:265659 )

N • no pathological alterations are seen in the brain

abnormal kinocilium morphology ( J:265659 )

• kinocilia are abnormally short in the utricles at E15 and E16

abnormal outer hair cell stereociliary bundle morphology ( J:265659 )

• most outer hair cells lack stereocilia at P6

• organ of Corti cultures from P1 mice show degeneration of outer hair cell stereocilia

• organ of Corti cultures from P1 mice treated with the HDAC inhibitors FK228, SAHA, or Merck60 show prevention of outer hair cell stereocilia degeneration

• mice injected with the HDAC inhibitor SAHA at P2, P4, and P5 show retention of stereocilia in outer hair cells

• SAHA treatment is more effective at rescuing the outer hair cells at P6 than at P16

cochlear hair cell degeneration ( J:265659 )

• hair cells are present until E16-17 but degenerate throughout the inner ear by P12

• the hearing organ of P6 mice shows increased apoptosis in hair cells

• the caspase inhibitors Z-DEVD-FMK and Z-VAD-FMK delay the death of hair cells in organ of Corti cultures but do not prevent the degeneration of stereocilia

cochlear inner hair cell degeneration ( J:265659 )

Genotype
MGI:6241489

cn3

• Allelic Composition: Rest^tm1.1Yasu/Rest⁺; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * C57BL/6J * CBA/J

mortality/aging

mortality/aging ( J:230341 )

N • mice are viable and survive to adulthood

pigmentation

belly spot ( J:230341 )

• ~60% of mice exhibit a white belly spot of variable size ranging from a few white hairs to a white spot that is ~1 cm in diameter; in contrast, only ~15% of control mice carrying the transgene alone form an extremely small, striated white-haired area on the belly

integument

belly spot ( J:230341 )

• ~60% of mice exhibit a white belly spot of variable size ranging from a few white hairs to a white spot that is ~1 cm in diameter; in contrast, only ~15% of control mice carrying the transgene alone form an extremely small, striated white-haired area on the belly

nervous system

nervous system phenotype ( J:230341 )

N • at E16.5, E18.5 and P0, no significant differences are observed in the number or distribution pattern of melanoblasts in the epidermis of vibrissae, back or abdominal skin relative to control mice

Genotype
MGI:6241536

cn4

• Allelic Composition: Rest^tm1.1Yasu/Rest⁺; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺; Tg(Dct-lacZ)A12Jkn/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

embryo

abnormal melanoblast morphology ( J:230341 )

• adult mice show a marked reduction in the number of Dct-LacZ+ melanoblasts and/or melanocytes in the white spot of abdominal skin

nervous system

abnormal melanoblast morphology ( J:230341 )

• adult mice show a marked reduction in the number of Dct-LacZ+ melanoblasts and/or melanocytes in the white spot of abdominal skin

Genotype
MGI:6241529

cn5

• Allelic Composition: Rest^tm1.1Yasu/Rest⁺; Tg(KRT14-cre)1Cgn/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * DBA/2

pigmentation

pigmentation phenotype ( J:230341 )

N • mice never form white spots

Genotype
MGI:6241517

cn6

• Allelic Composition: Rest^tm1.1Yasu/Rest⁺; Tg(Tyr-cre)1Lru/Y
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * DBA/2

pigmentation

pigmentation phenotype ( J:230341 )

♂N • mice never form white spots including on the belly region

Genotype
MGI:6241492

cn7

• Allelic Composition: Rest^tm1.1Yasu/Rest⁺; Tg(Mpz-cre)26Mes/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB/NTac

pigmentation

pigmentation phenotype ( J:230341 )

N • mice never form white spots including on the belly region

Genotype
MGI:6378689

cn8

• Allelic Composition: Rest^tm1.1Bban/Rest⁺; Gfi1^tm1(cre)Gan/Gfi1⁺
• Genetic Background: involves: 129S4/SvJaeSor * 129S7/SvEvBrd * FVB/N

hearing/vestibular/ear

cochlear inner hair cell degeneration ( J:265659 )

deafness ( J:265659 )

nervous system

cochlear inner hair cell degeneration ( J:265659 )

Genotype
MGI:6378690

cn9

• Allelic Composition: Rest^tm1.1Bban/Rest⁺; Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S4/SvJaeSor * C57BL/6J

hearing/vestibular/ear

cochlear hair cell degeneration ( J:265659 )

• tamoxifen-injected mice show partial loss of hair cells

• organ of Corti cultures from P5 mice incubated with tamoxifen and treated with the HDAC inhibitors FK228 or SAHA show prevention of the tamoxifen-dependent degeneration of inner hair cells and outer hair cells

• treatment of P7-P9 tamoxifen-injected mice with SAHA fromP7 to P15 reduces the extent of tamoxifen-dependent hair cell loss in cochleas

increased or absent threshold for auditory brainstem response ( J:265659 )

• in P70-P80 mice treated with tamoxifen from P40 to P47

impaired hearing ( J:265659 )

• mice treated with tamoxifen from P40 to P47 show hearing loss one month after treatment

• mice treated with tamoxifen from P7 to P9 show hearing loss at P16

• treatment of P7-P9 tamoxifen-injected mice with SAHA from P7 to P15 reduces the tamoxifen-dependent shift in hearing threshold at low sound frequencies

nervous system

cochlear hair cell degeneration ( J:265659 )

• tamoxifen-injected mice show partial loss of hair cells

• organ of Corti cultures from P5 mice incubated with tamoxifen and treated with the HDAC inhibitors FK228 or SAHA show prevention of the tamoxifen-dependent degeneration of inner hair cells and outer hair cells

• treatment of P7-P9 tamoxifen-injected mice with SAHA fromP7 to P15 reduces the extent of tamoxifen-dependent hair cell loss in cochleas

Genotype
MGI:6241494

cn10

• Allelic Composition: Dct^tm1(cre)Bee/Dct⁺; Rest^tm1.1Yasu/Rest⁺
• Genetic Background: involves: 129/Sv * 129S4/SvJae * C57BL/6

pigmentation

pigmentation phenotype ( J:230341 )

N • mice never form white spots including on the belly region