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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Msh2tm1Rak
targeted mutation 1, Raju Kucherlapati
MGI:2151801
Summary 8 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Msh2tm1Rak/Msh2tm1Rak involves: 129P2/OlaHsd MGI:4355511
hm2
 		Msh2tm1Rak/Msh2tm1Rak involves: 129P2/OlaHsd * C57BL/6 MGI:4355514
ht3
 		Msh2tm1Rak/Msh2+ involves: 129P2/OlaHsd MGI:4355512
cn4
 		Msh2tm1Rak/Msh2tm2.1Rak
Tg(Vil1-cre)20Syr/0 		involves: 129/Sv * 129P2/OlaHsd * C57BL/6 * DBA/2 * SJL MGI:4460268
cx5
 		Apctm1Rak/Apc+
Msh2tm1Rak/Msh2tm1Rak 		involves: 129P2/OlaHsd MGI:4355516
cx6
 		Msh2tm1Rak/Msh2tm1Rak
Poletm1.1Bdp/Poletm1.1Bdp 		involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J MGI:4366925
cx7
 		Msh2tm1Rak/Msh2tm1Rak
Pold1tm1.1Bdp/Pold1tm1.1Bdp 		involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J MGI:4366926
cx8
 		Msh2tm1Rak/Msh2tm1Rak
Xpctm1Ecf/Xpctm1Ecf 		involves: 129P2/OlaHsd * SKH1 MGI:4355517


Genotype
MGI:4355511
hm1
Allelic
Composition		 Msh2tm1Rak/Msh2tm1Rak
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Msh2tm1Rak mutation (1 available); any Msh2 mutation (95 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:64667 )
• homozygotes become moribund between 2.5 and 11.5 months of age

neoplasm
increased gastrointestinal tumor incidence ( J:64667 )
• 72% with intestinal tumors
• 4 mice with intestinal tumors but no lymphoma
• 22 of 36 mice with at least one intestinal tumor in addition to lymphoma
increased lymphoma incidence ( J:64667 )
• 72% with lymphoma
• 22 of 36 mice with at least one intestinal tumor in addition to lymphoma

immune system
abnormal class switch recombination ( J:56764 )
• reduction in isotype switching, particularly to IgG2b
decreased mature B cell number ( J:56764 )
• small reduction in the number of B cells

hematopoietic system
abnormal class switch recombination ( J:56764 )
• reduction in isotype switching, particularly to IgG2b
decreased mature B cell number ( J:56764 )
• small reduction in the number of B cells

digestive/alimentary system
increased gastrointestinal tumor incidence ( J:64667 )
• 72% with intestinal tumors
• 4 mice with intestinal tumors but no lymphoma
• 22 of 36 mice with at least one intestinal tumor in addition to lymphoma




Genotype
MGI:4355514
hm2
Allelic
Composition		 Msh2tm1Rak/Msh2tm1Rak
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Msh2tm1Rak mutation (1 available); any Msh2 mutation (95 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:98929 )
• homozygotes become moribund between 2.5 and 11.5 months of age
• DES treatment has no additional effect on morbidity

neoplasm
increased lymphoma incidence ( J:98929 )
• 58% of homozygotes develop lymphoma
• onset of lymphoma 154-185 days




Genotype
MGI:4355512
ht3
Allelic
Composition		 Msh2tm1Rak/Msh2+
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Msh2tm1Rak mutation (1 available); any Msh2 mutation (95 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
neoplasm
increased tumor incidence ( J:64667 )
• 11 of 16 heterozygotes develop a single tumor by 16-20 months of age
• additional animals with both a lymphoma and a hepatocellular adenoma
increased liver adenoma incidence ( J:64667 )
• 2 of 16 have hepatocellular adenomas
increased lymphoma incidence ( J:64667 )
• 8 of 16 with a lymphoma

digestive/alimentary system
colon polyps ( J:64667 )
• one mouse with a small colonic polyp

liver/biliary system
increased liver adenoma incidence ( J:64667 )
• 2 of 16 have hepatocellular adenomas




Genotype
MGI:4460268
cn4
Allelic
Composition		 Msh2tm1Rak/Msh2tm2.1Rak
Tg(Vil1-cre)20Syr/0
Genetic
Background		 involves: 129/Sv * 129P2/OlaHsd * C57BL/6 * DBA/2 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Msh2tm1Rak mutation (1 available); any Msh2 mutation (95 available)
Msh2tm2.1Rak mutation (1 available); any Msh2 mutation (95 available)
Tg(Vil1-cre)20Syr mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
neoplasm
increased gastrointestinal tumor incidence ( J:161577 )
• beginning at 6 months, mice develop intestinal tumors
• by 10 months, 50% of mice develop intestinal tumors
abnormal tumor morphology ( J:161577 )
• mice develop fewer and larger tumors than in Msh2tm2.1Rak/Msh2tm3.1Wed Tg(Vil-cre)20Syr mice
• tumors do not respond to treatment with cisplatin or FOLFOX unlike tumors from Msh2tm2.1Rak/Msh2tm3.1Wed Tg(Vil-cre)20Syr mice

homeostasis/metabolism
abnormal mismatch repair ( J:161577 )
• tumor cells exhibit increased microsatellite instability compared with wild-type cells
abnormal physiological response to xenobiotic ( J:161577 )
• tumors do not respond to treatment with cisplatin or FOLFOX unlike tumors from Msh2tm2.1Rak/Msh2tm3.1Wed Tg(Vil-cre)20Syr mice
• FOLFOX-treated intestinal mucosa exhibit decreased apoptosis compared with similarly treated cells from Msh2tm2.1Wed homozygotes and Msh2tm2.1Rak/Msh2tm3.1Wed Tg(Vil-cre)20Syr mice

cellular
abnormal mismatch repair ( J:161577 )
• tumor cells exhibit increased microsatellite instability compared with wild-type cells

digestive/alimentary system
increased gastrointestinal tumor incidence ( J:161577 )
• by 10 months, 50% of mice develop intestinal tumors
• beginning at 6 months, mice develop intestinal tumors

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
Lynch syndrome DOID:3883 OMIM:PS120435
 J:161577




Genotype
MGI:4355516
cx5
Allelic
Composition		 Apctm1Rak/Apc+
Msh2tm1Rak/Msh2tm1Rak
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apctm1Rak mutation (1 available); any Apc mutation (154 available)
Msh2tm1Rak mutation (1 available); any Msh2 mutation (95 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:64667 )
• mortality at 2-3 months of age

neoplasm
increased tumor incidence ( J:64667 )
• average of 81 tumors/mouse




Genotype
MGI:4366925
cx6
Allelic
Composition		 Msh2tm1Rak/Msh2tm1Rak
Poletm1.1Bdp/Poletm1.1Bdp
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Msh2tm1Rak mutation (1 available); any Msh2 mutation (95 available)
Poletm1.1Bdp mutation (0 available); any Pole mutation (99 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, complete penetrance ( J:153684 )
• no mice are present at weaning




Genotype
MGI:4366926
cx7
Allelic
Composition		 Msh2tm1Rak/Msh2tm1Rak
Pold1tm1.1Bdp/Pold1tm1.1Bdp
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Msh2tm1Rak mutation (1 available); any Msh2 mutation (95 available)
Pold1tm1.1Bdp mutation (0 available); any Pold1 mutation (62 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, complete penetrance ( J:153684 )
• no mice are present at weaning




Genotype
MGI:4355517
cx8
Allelic
Composition		 Msh2tm1Rak/Msh2tm1Rak
Xpctm1Ecf/Xpctm1Ecf
Genetic
Background		 involves: 129P2/OlaHsd * SKH1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Msh2tm1Rak mutation (1 available); any Msh2 mutation (95 available)
Xpctm1Ecf mutation (1 available); any Xpc mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal cell cycle ( J:94447 )
• fewer epidermal cells accumulate in S-phase and in G2-phase after treatment with 500J/m2 of UV radiation than in mice carrying Xpctm1Ecf alone




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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory