Pold1^tm1.1Bdp
Targeted Allele Detail

Summary

• Symbol: Pold1^tm1.1Bdp
• Name: polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 1.1, Bradley D Preston
• MGI ID: MGI:4458032
• Synonyms: Pold1^e
• Gene: Pold1 Location: Chr7:44182168-44198239 bp, - strand Genetic Position: Chr7, 28.83 cM
• Alliance: Pold1^tm1.1Bdp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153684
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: A point mutation resulting in an aspartic acid to alanine substition at residue 400 (D400A) was introduced at the endogenous locus by homologous recombination of a targeting vector also carrying an intronic neo cassette. The neo cassette was removed by cre-mediated recombination. The missense mutation was introduced into sequence encoding the proofreading domain. (J:100685)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pold1^tm1.1Bdp

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pold1 Mutation: 62 strains or lines available

References

• Original: J:153684 Albertson TM, et al., DNA polymerase epsilon and delta proofreading suppress discrete mutator and cancer phenotypes in mice. Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):17101-4
• All: 3 reference(s)