Phenotypes associated with this allele

• Allele Symbol: Lat^tm1Les
• Allele Name: targeted mutation 1, Lawrence E Samelson
• Allele ID: MGI:1857812
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lat^tm1Les/Lat^tm1Les	Not Specified	MGI:2181663
ht2	Lat^tm1Les/Lat^tm1.1Wz	involves: C57BL/6	MGI:3843486
ht3	Lat^tm1Les/Lat^tm1.2Wz	involves: C57BL/6 * FVB/N	MGI:3843487
cn4	Lat^tm1Les/Lat^tm1.1Wz Tg(Cd4-cre)1Cwi/0	involves: C57BL/6 * DBA/2	MGI:3843484
cx5	Cbl^tm1.1Hua/Cbl^tm1.1Hua Lat^tm1Les/Lat^tm1Les	involves: 129P2/OlaHsd	MGI:3626257
cx6	Lat^tm1Les/Lat^tm1Les Lcp2^tm5(Lat/Lcp2)Gak/Lcp2^tm5(Lat/Lcp2)Gak	involves: 129S1/Sv * 129X1/SvJ	MGI:3842654
cx7	Lat^tm1Les/Lat^tm1Les Lat2^tm1Wz/Lat2^tm1Wz	involves: C57BL/6	MGI:3511020
cx8	Lat^tm1Les/Lat^tm1Les Tg(CD2-LAT2)1Wz/0	Not Specified	MGI:3695391

Genotype
MGI:2181663

hm1

• Allelic Composition: Lat^tm1Les/Lat^tm1Les
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

small thymus ( J:54059 )

• thymus is very small compared to wild-type

abnormal T cell morphology ( J:54059 )

• null mice do not have T cells

arrested T cell differentiation ( J:54059 , J:116964 )

• in null mice, thymocytes are arrested at the double negative to double positive transition (J:54059)

decreased T cell number ( J:54059 )

• null mice are missing all subsets of mature TCR+ lymphocytes

decreased thymocyte number ( J:54059 )

• thymocyte numbers are 10-20x lower than in wild-type or heterozygotes

decreased double-negative T cell number ( J:54059 )

• double negative alpha beta TCR+ cells are absent in mutants

hematopoietic system

small thymus ( J:54059 )

• thymus is very small compared to wild-type

abnormal T cell morphology ( J:54059 )

• null mice do not have T cells

arrested T cell differentiation ( J:54059 , J:116964 )

• in null mice, thymocytes are arrested at the double negative to double positive transition (J:54059)

decreased T cell number ( J:54059 )

• null mice are missing all subsets of mature TCR+ lymphocytes

decreased thymocyte number ( J:54059 )

• thymocyte numbers are 10-20x lower than in wild-type or heterozygotes

decreased double-negative T cell number ( J:54059 )

• double negative alpha beta TCR+ cells are absent in mutants

endocrine/exocrine glands

small thymus ( J:54059 )

• thymus is very small compared to wild-type

decreased thymocyte number ( J:54059 )

• thymocyte numbers are 10-20x lower than in wild-type or heterozygotes

Genotype
MGI:3843486

ht2

• Allelic Composition: Lat^tm1Les/Lat^tm1.1Wz
• Genetic Background: involves: C57BL/6

hematopoietic system

abnormal effector T cell morphology ( J:147946 )

• the percentage of mature T cells in the periphery is lower than in wild-type mice

• however, thymocyte development is otherwise normal

immune system

abnormal effector T cell morphology ( J:147946 )

• the percentage of mature T cells in the periphery is lower than in wild-type mice

• however, thymocyte development is otherwise normal

Genotype
MGI:3843487

ht3

• Allelic Composition: Lat^tm1Les/Lat^tm1.2Wz
• Genetic Background: involves: C57BL/6 * FVB/N

immune system

arrested T cell differentiation ( J:147946 )

• T cell development is arrested at DN1

absent T cells ( J:147946 )

• mice lack mature T cells in the periphery

hematopoietic system

arrested T cell differentiation ( J:147946 )

• T cell development is arrested at DN1

absent T cells ( J:147946 )

• mice lack mature T cells in the periphery

Genotype
MGI:3843484

cn4

• Allelic Composition: Lat^tm1Les/Lat^tm1.1Wz; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

small thymus ( J:147946 )

decreased thymocyte number ( J:147946 )

abnormal T cell differentiation ( J:147946 )

• based on marker expression, thymocyte positive selection is impaired and single positive thymocyte development is blocked unlike in wild-type mice

increased double-positive T cell number ( J:147946 )

• the percentage of double positive thymocytes is slightly increased compared to in wild-type mice

• however, the absolute numbers of double positive thymocytes are normal

increased gamma-delta T cell number ( J:147946 )

• the percentage of gamma delta T cells is increased compared to in wild-type mice

decreased regulatory T cell number ( J:147946 )

• few, if any

abnormal effector T cell morphology ( J:147946 )

• the number of mature T cells is decreased compared to in wild-type mice

• few mature T cells are GFP+

decreased CD4-positive, alpha-beta T cell number ( J:147946 )

• the number of CD4+ T cells is decreased 10-fold compared to in wild-type mice

• the CD4+ T cells that remain are GFP-

decreased CD8-positive, alpha-beta T cell number ( J:147946 )

• the number of CD8+ T cells is decreased 20-fold compared to in wild-type mice

• the CD8+ T cells that remain are GFP-

• however, the number of immature single positive cells is normal

abnormal T cell physiology ( J:147946 )

• TCR-mediated calcium fluxes are abrogated unlike in wild-type mice

hematopoietic system

small thymus ( J:147946 )

decreased thymocyte number ( J:147946 )

abnormal T cell differentiation ( J:147946 )

• based on marker expression, thymocyte positive selection is impaired and single positive thymocyte development is blocked unlike in wild-type mice

increased double-positive T cell number ( J:147946 )

• the percentage of double positive thymocytes is slightly increased compared to in wild-type mice

• however, the absolute numbers of double positive thymocytes are normal

increased gamma-delta T cell number ( J:147946 )

• the percentage of gamma delta T cells is increased compared to in wild-type mice

decreased regulatory T cell number ( J:147946 )

• few, if any

abnormal effector T cell morphology ( J:147946 )

• the number of mature T cells is decreased compared to in wild-type mice

• few mature T cells are GFP+

decreased CD4-positive, alpha-beta T cell number ( J:147946 )

• the number of CD4+ T cells is decreased 10-fold compared to in wild-type mice

• the CD4+ T cells that remain are GFP-

decreased CD8-positive, alpha-beta T cell number ( J:147946 )

• the number of CD8+ T cells is decreased 20-fold compared to in wild-type mice

• the CD8+ T cells that remain are GFP-

• however, the number of immature single positive cells is normal

abnormal T cell physiology ( J:147946 )

• TCR-mediated calcium fluxes are abrogated unlike in wild-type mice

endocrine/exocrine glands

small thymus ( J:147946 )

decreased thymocyte number ( J:147946 )

Genotype
MGI:3626257

cx5

• Allelic Composition: Cbl^tm1.1Hua/Cbl^tm1.1Hua; Lat^tm1Les/Lat^tm1Les
• Genetic Background: involves: 129P2/OlaHsd

hematopoietic system

abnormal thymus development ( J:91369 )

• Cbl inactivation in Lat deficient mice partially rescues thymic development; ~24% double positive and and 7% CD4 single positive cells are detected in the thymi of double mutants but not in Lat-null animals

thymus hypoplasia ( J:91369 )

• thymus cellularity of double knockout mice is 5% that of wild-type

increased CD4-positive, alpha-beta T cell number ( J:91369 )

• mutants have elevated levels of CD4+ T cells in the spleen and lymph nodes of 6 week old mice compared to wild-type or Cbl-null mice

immune system

abnormal thymus development ( J:91369 )

• Cbl inactivation in Lat deficient mice partially rescues thymic development; ~24% double positive and and 7% CD4 single positive cells are detected in the thymi of double mutants but not in Lat-null animals

thymus hypoplasia ( J:91369 )

• thymus cellularity of double knockout mice is 5% that of wild-type

increased CD4-positive, alpha-beta T cell number ( J:91369 )

• mutants have elevated levels of CD4+ T cells in the spleen and lymph nodes of 6 week old mice compared to wild-type or Cbl-null mice

liver inflammation ( J:91369 )

• similar to what is seen in Lcp Cbl double null mice

lung inflammation ( J:91369 )

• similar to what is seen in Lcp Cbl double null mice

liver/biliary system

liver inflammation ( J:91369 )

• similar to what is seen in Lcp Cbl double null mice

respiratory system

lung inflammation ( J:91369 )

• similar to what is seen in Lcp Cbl double null mice

endocrine/exocrine glands

abnormal thymus development ( J:91369 )

• Cbl inactivation in Lat deficient mice partially rescues thymic development; ~24% double positive and and 7% CD4 single positive cells are detected in the thymi of double mutants but not in Lat-null animals

thymus hypoplasia ( J:91369 )

• thymus cellularity of double knockout mice is 5% that of wild-type

Genotype
MGI:3842654

cx6

• Allelic Composition: Lat^tm1Les/Lat^tm1Les; Lcp2^{tm5(Lat/Lcp2)Gak}/Lcp2^{tm5(Lat/Lcp2)Gak}
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

immune system

decreased double-positive T cell number ( J:147721 )

• double-positive thymocytes are absent in these mice

arrested T cell differentiation ( J:147721 )

• thymocytes are arrested at the DN3 stage

hematopoietic system

decreased double-positive T cell number ( J:147721 )

• double-positive thymocytes are absent in these mice

arrested T cell differentiation ( J:147721 )

• thymocytes are arrested at the DN3 stage

Genotype
MGI:3511020

cx7

• Allelic Composition: Lat^tm1Les/Lat^tm1Les; Lat2^tm1Wz/Lat2^tm1Wz
• Genetic Background: involves: C57BL/6

immune system

abnormal mast cell physiology ( J:93951 )

• granules are not released following antigen stimulation similar to Lat^tm1Les homozygotes

hematopoietic system

abnormal mast cell physiology ( J:93951 )

• granules are not released following antigen stimulation similar to Lat^tm1Les homozygotes

Genotype
MGI:3695391

cx8

• Allelic Composition: Lat^tm1Les/Lat^tm1Les; Tg(CD2-LAT2)1Wz/0
• Genetic Background: Not Specified

immune system

small thymus ( J:116964 )

• at 4 weeks of age; however, thymus size increases with age

enlarged spleen ( J:116964 )

• at 4 weeks of age

spleen hyperplasia ( J:116964 )

• increase in the total number of B and T cells by 4 weeks of age

abnormal T cell differentiation ( J:116964 )

• partial block in T cell development, with most cells stopping at double negative stage 3

• however, unlike in Lat single homozygotes some T cell do make it past the double negative stage

decreased double-positive T cell number ( J:116964 )

• at 10 weeks of age the double positive cell population is practically absent from the thymus

abnormal single-positive T cell number ( J:116964 )

• a 3- to 20-fold increase in the ratio of CD4 to CD8 cells is seen in cells from the spleen and lymph nodes at 4 weeks of age

• increase in CD4 to CD8 ratio is larger in older mice

abnormal spleen periarteriolar lymphoid sheath morphology ( J:116964 )

• T cell organization is disrupted

abnormal leukocyte physiology ( J:116964 )

• high rate of splenocyte proliferation in the absence of stimulation; however, with stimulation proliferation level is similar to wild-type indicating no increase in maximal proliferative capacity

abnormal B cell physiology ( J:116964 )

• a decrease in the proportion of resting B cell and an increase in the proportion of activated B cells is seen in the spleen

increased IgG1 level ( J:116964 )

• marked increase in IgG1 but no significant differences in the levels of IgG2a, IgG2b, IgG3 or IgA compared to wild-type mice

increased IgM level ( J:116964 )

• moderately increased

abnormal interferon secretion ( J:116964 )

• production of IFNG is seen only after stimulation with phorbol myristate acetate (PMA) and ionomycin and not when splenocytes are stimulated with anti-CD3 and anti-CD28

abnormal interleukin secretion ( J:116964 )

• production of IL-2 is seen only after stimulation with PMA and ionomycin and not when splenocytes are stimulated with anti-CD3 and anti-CD28

increased interleukin-10 secretion ( J:116964 )

• increased splenocyte production of IL-10 after stimulation with PMA and ionomycin

increased interleukin-4 secretion ( J:116964 )

• splenocytes produce large amounts of IL-4

hematopoietic system

small thymus ( J:116964 )

• at 4 weeks of age; however, thymus size increases with age

enlarged spleen ( J:116964 )

• at 4 weeks of age

spleen hyperplasia ( J:116964 )

• increase in the total number of B and T cells by 4 weeks of age

abnormal T cell differentiation ( J:116964 )

• partial block in T cell development, with most cells stopping at double negative stage 3

• however, unlike in Lat single homozygotes some T cell do make it past the double negative stage

decreased double-positive T cell number ( J:116964 )

• at 10 weeks of age the double positive cell population is practically absent from the thymus

abnormal single-positive T cell number ( J:116964 )

• a 3- to 20-fold increase in the ratio of CD4 to CD8 cells is seen in cells from the spleen and lymph nodes at 4 weeks of age

• increase in CD4 to CD8 ratio is larger in older mice

abnormal spleen periarteriolar lymphoid sheath morphology ( J:116964 )

• T cell organization is disrupted

abnormal leukocyte physiology ( J:116964 )

• high rate of splenocyte proliferation in the absence of stimulation; however, with stimulation proliferation level is similar to wild-type indicating no increase in maximal proliferative capacity

abnormal B cell physiology ( J:116964 )

• a decrease in the proportion of resting B cell and an increase in the proportion of activated B cells is seen in the spleen

increased IgG1 level ( J:116964 )

• marked increase in IgG1 but no significant differences in the levels of IgG2a, IgG2b, IgG3 or IgA compared to wild-type mice

increased IgM level ( J:116964 )

• moderately increased

endocrine/exocrine glands

small thymus ( J:116964 )

• at 4 weeks of age; however, thymus size increases with age

growth/size/body

enlarged spleen ( J:116964 )

• at 4 weeks of age

spleen hyperplasia ( J:116964 )

• increase in the total number of B and T cells by 4 weeks of age