Lcp2^{tm5(Lat/Lcp2)Gak}
Targeted Allele Detail

Summary

• Symbol: Lcp2^{tm5(Lat/Lcp2)Gak}
• Name: lymphocyte cytosolic protein 2; targeted mutation 5, Gary A Koretzky
• MGI ID: MGI:3842651
• Synonyms: Membrane-targeted SLP-76, MTS
• Gene: Lcp2 Location: Chr11:33996928-34042281 bp, + strand Genetic Position: Chr11, 19.43 cM
• Alliance: Lcp2^{tm5(Lat/Lcp2)Gak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147721
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An allele expressing a chimeric molecule containing the membrane and lipid raft-targeting sequences of LAT fused to the full-length Lcp2 (SLP-76) was created in the following manner. Base pairs 1-105 of murine Lat cDNA were fused to exon 1 and intron 1 of Lcp2. The ATG start site of the Lcp2 gene was mutated and the entire construct was placed next to a FRT-flanked neomycin cassette. After homologous recombination, mice carrying the correctly targeted allele were crossed with FLPe transgenic mice to remove the neomycin cassette. Immunoblot analysis of heterozygote thymocytes detected the 80 kDA chimeric protein and the 76 kDa wild-type protein. (J:147721)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lcp2 Mutation: 41 strains or lines available

References

• Original: J:147721 Bezman NA, et al., Cutting edge: Rescue of pre-TCR but not mature TCR signaling in mice expressing membrane-targeted SLP-76. J Immunol. 2009 May 1;182(9):5183-7
• All: 2 reference(s)