Htra1^tm1Ybf
Targeted Allele Detail

Summary

• Symbol: Htra1^tm1Ybf
• Name: HtrA serine peptidase 1; targeted mutation 1, Yingbin Fu
• MGI ID: MGI:5285550
• Gene: Htra1 Location: Chr7:130537933-130587388 bp, + strand Genetic Position: Chr7, 73.2 cM
• Alliance: Htra1^tm1Ybf page

Similar development of polypoidal choroidal vasculopathy in Htra1^tm1Ybf/Htra1^tm1Ybf Tg(BEST1-HTRA1)#Ybf/0 mice as in Tg(BEST1-HTRA1)#Ybf/0 mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:175227
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Part of exon 1 including the ATG initiation codon was replaced with an IRES-lacZ reporter and neomycin-resistance cassette via homologous recombination. Northern blot and RT-PCR analysis confirmed the absence of expression in brains and retinas from homozygous mice (J:175227)

Targeting strategy for the generation of the Htra1^tm1Ybf allele

Expression

In Mice Carrying this Mutation:

24 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Htra1 Mutation: 50 strains or lines available

References

• Original: J:175227 Jones A, et al., Increased expression of multifunctional serine protease, HTRA1, in retinal pigment epithelium induces polypoidal choroidal vasculopathy in mice. Proc Natl Acad Sci U S A. 2011 Aug 30;108(35):14578-83
• All: 11 reference(s)