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Gene Expression Data
Assay Details
Assay
Reference: J:279716 Braitsch CM, et al., LATS1/2 suppress NFkappaB and aberrant EMT initiation to permit pancreatic progenitor differentiation. PLoS Biol. 2019 Jul;17(7):e3000382
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8346997
Gene symbol: Gcg
Gene name: glucagon
Antibody: anti-Glucagon (N-17) (sc-7780)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were listed in the methods: MGI:8346363 or MGI:1343940; either antibody could have been used in this assay.
Results
Specimen 1C: postnatal day 1 (more )
Note: Controls were either wild type or heterozygous littermates. Triple labeled: red - Gcg; white - Cdh1 (assay MGI:8346384); green - Ins1 (assay MGI:8347766) and Ins2 (assay MGI:8347767).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Present 1C
Specimen 1G: postnatal day 1; Lats1tm1.1Jfm/Lats1tm1.1Jfm, Lats2tm1.1Jfm/Lats2tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Triple labeled: red - Gcg; white - Cdh1 (assay MGI:8346384); green - Ins1 (assay MGI:8347766) and Ins2 (assay MGI:8347767).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Absent 1G
Specimen 1K: embryonic day 15.5 (more )
Note: Controls were either wild type or heterozygous littermates. Double labeled: red - Gcg; white - Cdh1 (assay MGI:8346384).
Structure Cell Type Level Pattern Image Note
TS23: pancreas pancreatic A cell Present 1K
Specimen 1L: embryonic day 15.5; Lats1tm1.1Jfm/Lats1tm1.1Jfm, Lats2tm1.1Jfm/Lats2tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Double labeled: red - Gcg; white - Cdh1 (assay MGI:8346384).
Structure Cell Type Level Pattern Image Note
TS23: pancreas pancreatic A cell Absent 1L
Specimen 1M: embryonic day 12.5 (more )
Note: Controls were either wild type or heterozygous littermates. Triple labeled: red - Gcg; white - Cdh1 (assay MGI:8346384); green - Yap1 (assay MGI:8347770).
Structure Cell Type Level Pattern Image Note
TS20: pancreas pancreatic A cell Present 1M left
Specimen 1N: embryonic day 12.5; Lats1tm1.1Jfm/Lats1tm1.1Jfm, Lats2tm1.1Jfm/Lats2tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Triple labeled: red - Gcg; white - Cdh1 (assay MGI:8346384); green - Yap1 (assay MGI:8347770).
Structure Cell Type Level Pattern Image Note
TS20: pancreas pancreatic A cell Absent 1N left
Specimen 1O: embryonic day 12.5; Lats1tm1.1Jfm/Lats1tm1.1Jfm, Lats2tm1.1Jfm/Lats2tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0, Wwtr1tm1.1Eno/Wwtr1+, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Triple labeled: red - Gcg; white - Cdh1 (assay MGI:8346384); green - Yap1 (assay MGI:8347770).
Structure Cell Type Level Pattern Image Note
TS20: pancreas pancreatic A cell Absent 1O left
Specimen S1D WT E11.5: embryonic day 11.5 (more )
Note: Controls were either wild type or heterozygous littermates. Triple labeled: magenta - Gcg and Ins1 and Ins2; green - Cdh1 (assay MGI:8346384); red - Muc1 (assay MGI:8346383).
Structure Cell Type Level Pattern Image Note
TS19: pancreas primordium pancreatic A cell Present Clusters S1D WT upper In WT pancreas, clusters of insulin-positive/glucagon-positive (beta + alpha) endocrine cells, intercalated between epithelial branches, were evident at E11.5 and E12.5.
Specimen S1D Lats1/2PanKO E11.5: embryonic day 11.5; Lats1tm1.1Jfm/Lats1tm1.1Jfm, Lats2tm1.1Jfm/Lats2tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Triple labeled: magenta - Gcg and Ins1 and Ins2; green - Cdh1 (assay MGI:8346384); red - Muc1 (assay MGI:8346383).
Structure Cell Type Level Pattern Image Note
TS19: pancreas primordium epithelium pancreatic A cell Present Non-Uniform S1D Lats1/2PanKO upper Early insulin/glucagon-positive cells were dispersed within the abnormally thickened Lats1/2<PanKO> epithelium at E11.5.
Specimen S1D WT E12.5: embryonic day 12.5 (more )
Note: Controls were either wild type or heterozygous littermates. Triple labeled: magenta - Gcg and Ins1 and Ins2; green - Cdh1 (assay MGI:8346384); red - Muc1 (assay MGI:8346383).
Structure Cell Type Level Pattern Image Note
TS20: pancreas pancreatic A cell Present Clusters S1D WT lower In WT pancreas, clusters of insulin-positive/glucagon-positive (beta + alpha) endocrine cells, intercalated between epithelial branches, were evident at E11.5 and E12.5.
Specimen S1D Lats1/2PanKO E12.5: embryonic day 12.5; Lats1tm1.1Jfm/Lats1tm1.1Jfm, Lats2tm1.1Jfm/Lats2tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Triple labeled: magenta - Gcg and Ins1 and Ins2; green - Cdh1 (assay MGI:8346384); red - Muc1 (assay MGI:8346383).
Structure Cell Type Level Pattern Image Note
TS20: pancreas pancreatic A cell Absent S1D Lats1/2PanKO lower
Specimen S1F: embryonic day 10.5 (more )
Note: Controls were either wild type or heterozygous littermates. Triple labeled: red - Gcg; white - Cdh1 (assay MGI:8346384); green - Yap1 (assay MGI:8348237).
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1F left , S1F right
Specimen S1H: embryonic day 10.5; Lats1tm1.1Jfm/Lats1tm1.1Jfm, Lats2tm1.1Jfm/Lats2tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Triple labeled: red - Gcg; white - Cdh1 (assay MGI:8346384); green - Yap1 (assay MGI:8348237).
Structure Level Pattern Image Note
TS17: pancreas primordium Ambiguous S1H right , S1H left
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
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