Ins2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:279716 Braitsch CM, et al., LATS1/2 suppress NFkappaB and aberrant EMT initiation to permit pancreatic progenitor differentiation. PLoS Biol. 2019 Jul;17(7):e3000382
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8347767
Gene symbol:	Ins2
Gene name:	insulin II

Antibody:	Anti-Insulin (4590)
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Two antibodies were listed in the methods: MGI:8346364 or MGI:1343939; either antibody could have been used in this assay.

Results

1C ©

Specimen 1C: postnatal day 1 (more

)
Note: Controls were either wild type or heterozygous littermates. Triple labeled: green - Ins2 and Ins1; white - Cdh1 (assay MGI:8346384); red - Gcg (assay MGI:8346997).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: pancreas	type B pancreatic cell	Present		1C

1G ©

Specimen 1G: postnatal day 1; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Triple labeled: green - Ins2 and Ins1; white - Cdh1 (assay MGI:8346384); red - Gcg (assay MGI:8346997).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: pancreas	type B pancreatic cell	Absent		1G

S1D WT upper ©

Specimen S1D WT E11.5: embryonic day 11.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Triple labeled: magenta - Ins2 and Gcg and Ins1; green - Cdh1 (assay MGI:8346384); red - Muc1 (assay MGI:8346383).

Structure	Cell Type	Level	Pattern	Image	Note
TS19: pancreas primordium	type B pancreatic cell	Present	Clusters	S1D WT upper	In WT pancreas, clusters of insulin-positive/glucagon-positive (beta + alpha) endocrine cells, intercalated between epithelial branches, were evident at E11.5 and E12.5.

S1D Lats1/2^PanKO upper ©

Specimen S1D Lats1/2^PanKO E11.5: embryonic day 11.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Triple labeled: magenta - Ins2 and Gcg and Ins1; green - Cdh1 (assay MGI:8346384); red - Muc1 (assay MGI:8346383).

Structure	Cell Type	Level	Pattern	Image	Note
TS19: pancreas primordium epithelium	type B pancreatic cell	Present	Non-Uniform	S1D Lats1/2^PanKO upper	Early insulin/glucagon-positive cells were dispersed within the abnormally thickened Lats1/2<PanKO> epithelium at E11.5.

S1D WT lower ©

Specimen S1D WT E12.5: embryonic day 12.5 (more

)
Note: Controls were either wild type or heterozygous littermates. Triple labeled: magenta - Ins2 and Gcg and Ins1; green - Cdh1 (assay MGI:8346384); red - Muc1 (assay MGI:8346383).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: pancreas	type B pancreatic cell	Present	Clusters	S1D WT lower	In WT pancreas, clusters of insulin-positive/glucagon-positive (beta + alpha) endocrine cells, intercalated between epithelial branches, were evident at E11.5 and E12.5.

Specimen S1D Lats1/2^PanKO E12.5: embryonic day 12.5; Lats1^tm1.1Jfm/Lats1^tm1.1Jfm, Lats2^tm1.1Jfm/Lats2^tm1.1Jfm, Tg(Pdx1-cre)89.1Dam/0 (more

)
Note: Conditional mutant. Triple labeled: magenta - Ins2 and Gcg and Ins1; green - Cdh1 (assay MGI:8346384); red - Muc1 (assay MGI:8346383).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: pancreas	type B pancreatic cell	Absent		S1D Lats1/2^PanKO lower

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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