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Gene Expression Data
Assay Details
Assay
Reference: J:178173 Mastracci TL, et al., Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell development and endocrine hormone expression. Dev Biol. 2011 Nov 1;359(1):1-11
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8161242
Gene symbol: Gcg
Gene name: glucagon
Antibody: anti-glucagon
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1F: embryonic day 10.5; Nkx2-2tm3Suss/Nkx2-2+ (more )
Note: Double labeled: red - Gcg; green - Pdx1 (assay MGI:8161245).
Structure Cell Type Level Pattern Image Note
TS17: pancreas primordium pancreatic A cell Present 1F Compared with the Arx and lacZ staining in adjacent section (Fig 1G), coepression with Nkx2-2 or Arx was seen. There was also a population of Arx+/Nkx2-2+ co-expressing cells that did not express Gcg.
Specimen 1H: embryonic day 10.5; Nkx2-2tm3Suss/Nkx2-2tm3Suss (more )
Note: Double labeled: red - Gcg; green - Pdx1 (assay MGI:8161245).
Structure Cell Type Level Pattern Image Note
TS17: pancreas primordium pancreatic A cell Present 1H Compared with the Arx and lacZ staining in adjacent section (Fig 1I), coepression with Nkx2-2 or Arx was seen. There was also a population of Arx+/Nkx2-2+ co-expressing cells that did not express Gcg.
Specimen 1J: embryonic day 10.5; Nkx2-2tm3Suss/Nkx2-2+ (more )
Note: Double labeled: red - Gcg; green - Nkx2-2 reporter (assay MGI:8161244).
Structure Cell Type Level Pattern Image Note
TS17: pancreas primordium pancreatic A cell Present 1J Coexpression with Nkx2-2 was seen.
Specimen 1K: embryonic day 10.5; Nkx2-2tm3Suss/Nkx2-2+ (more )
Note: Double labeled: red - Gcg; green - Arx (assay MGI:8161243).
Structure Cell Type Level Pattern Image Note
TS17: pancreas primordium pancreatic A cell Present 1K Coexpression with Arx was seen.
Specimen 1L: embryonic day 10.5; Nkx2-2tm3Suss/Nkx2-2tm3Suss (more )
Note: Double labeled: red - Gcg; green - Nkx2-2 reporter (assay MGI:8161244).
Structure Cell Type Level Pattern Image Note
TS17: pancreas primordium pancreatic A cell Present 1L Coexpressed with Nkx2-2 in the remaining positive cells in the mutant.
Specimen 1M: embryonic day 10.5; Nkx2-2tm3Suss/Nkx2-2tm3Suss (more )
Note: Double labeled: red - Gcg; green - Arx (assay MGI:8161243).
Structure Cell Type Level Pattern Image Note
TS17: pancreas primordium pancreatic A cell Present 1M Coexpressed with Arx in the remaining positive cells in the mutant.
Specimen 2A: embryonic day 9.5 (more )
Note: Double labeled: red - Gcg; green - Pdx1 (assay MGI:8161245).
Structure Cell Type Level Pattern Image Note
TS15: pancreas primordium pancreatic A cell Present 2A
Specimen 2B: embryonic day 11.5 (more )
Note: Double labeled: red - Gcg; green - Pdx1 (assay MGI:8161245).
Structure Cell Type Level Pattern Image Note
TS19: pancreas primordium pancreatic A cell Present 2B
Specimen 2C: embryonic day 13.5 (more )
Note: Double labeled: red - Gcg; green - Pdx1 (assay MGI:8161245).
Structure Cell Type Level Pattern Image Note
TS21: pancreas pancreatic A cell Present 2C
Specimen 2D: embryonic day 15.5 (more )
Note: Double labeled: red - Gcg; green - Pdx1 (assay MGI:8161245).
Structure Cell Type Level Pattern Image Note
TS23: pancreas pancreatic A cell Present 2D
Specimen 4E: postnatal day 0 (more )
Note: Double labeled: red - Gcg; green - Ghrl (assay MGI:8161241).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Present 4E
Specimen 4F: postnatal day 0; Arxtm1Gldn/?, Tg(Pdx1-cre)6Tuv/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male. Double labeled: red - Gcg; green - Ghrl (assay MGI:8161241).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Present 4F Reduced number of positive cells, compared with the wild-type.
Specimen 4G: postnatal day 0; Nkx2-2tm1Jlr/Nkx2-2tm1Jlr (more )
Note: Double labeled: red - Gcg; green - Ghrl (assay MGI:8161241).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Present 4G Reduced number of positive cells, compared with the wild-type.
Specimen 4H: postnatal day 0; Arxtm1Gldn/?, Nkx2-2tm1Jlr/Nkx2-2tm1Jlr, Tg(Pdx1-cre)6Tuv/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male. Double labeled: red - Gcg; green - Ghrl (assay MGI:8161241).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Weak 4H An additive decrease was seen in the double mutants.
Specimen 6A: postnatal day 0 (more )
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Present 6A
Specimen 6B: postnatal day 0; Arxtm1Gldn/?, Tg(Neurog3-cre)C1Able/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male.
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Weak 6B Decreased expression compared with the wild-type.
Specimen 6C: postnatal day 0; Nkx2-2tm1Jlr/Nkx2-2tm1Jlr (more )
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Weak 6C Decreased expression compared with the wild-type.
Specimen 6D: postnatal day 0; Arxtm1Gldn/?, Nkx2-2tm1Jlr/Nkx2-2tm1Jlr, Tg(Neurog3-cre)C1Able/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male.
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic A cell Weak 6D Decreased expression compared with the wild-type, with an additive decrease in expression in the double mutant.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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