Gata6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:312380 Sun-Wada GH, et al., Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm. Sci Rep. 2021 Sep 29;11(1):19355
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7541116
Gene symbol:	Gata6
Gene name:	GATA binding protein 6

Antibody:	anti-GATA6
Detection system:	Secondary antibody coupled to Cy5

Results

2a ©

2b ©

Specimen 2a/b: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; red - Cdh1 (assay MGI:7541114); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2a , 2b	visceral endoderm

2e ©

2f ©

Specimen 2e/f: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; red - Cdh1 (assay MGI:7541114); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2e , 2f	Positive cells did not exhibit epithelial structure but they appeared as a clump.

2i ©

2j ©

Specimen 2i/j: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Cdh1 (assay MGI:7541117); red - Ezr (assay MGI:7541118).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2j , 2i	visceral endoderm

2m ©

2n ©

Specimen 2m/n: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Cdh1 (assay MGI:7541117); red - Ezr (assay MGI:7541118).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2m , 2n	Positive cells did not exhibit epithelial structure but they appeared as a clump.

2q ©

2r ©

Specimen 2q/r: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Atp1a1 (assay MGI:7541122); red - Ctnnb1 (assay MGI:7541119).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2r , 2q	visceral endoderm

2u ©

2v ©

Specimen 2u/v: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Atp1a1 (assay MGI:7541122); red - Ctnnb1 (assay MGI:7541119).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2u , 2v	Positive cells did not exhibit epithelial structure but they appeared as a clump.

3a ©

3b1 ©

3b2 ©

Specimen 3a/b: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; red - Atp6v0c (assay MGI:7541123); green - Lamp2 (assay MGI:7541124).

Structure	Level	Pattern	Image	Note
TS7: parietal endoderm	Present		3b2 , 3a
TS7: embryonic visceral endoderm	Present		3b2 , 3b1 , 3a

3c ©

3d1 ©

3d2 ©

Specimen 3c/d: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; red - Atp6v0c (assay MGI:7541123); green - Lamp2 (assay MGI:7541124).

Structure	Level	Pattern	Image	Note
TS7: parietal endoderm	Present		3c , 3d2
TS7: embryonic visceral endoderm	Present		3d2 , 3c , 3d1

4a ©

4b ©

Specimen 4a/b: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab7 (assay MGI:7541126).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4b , 4a

4e ©

4f ©

Specimen 4e/f: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab7 (assay MGI:7541126).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4e , 4f	Positive cells did not exhibit epithelial structure but they appeared as a clump.

4i ©

4j ©

Specimen 4i/j: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Stx7 (assay MGI:7541127).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4j , 4i

4m ©

4n ©

Specimen 4m/n: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Stx7 (assay MGI:7541127).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4n , 4m	Positive cells did not exhibit epithelial structure but they appeared as a clump.

5a ©

5a1 ©

5a2 ©

5a3 ©

Specimen 5a: embryonic day 5.5 (more

)
Note: Double labeled: blue - Gata6; red - Snx1 (assay MGI:7541128).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5a1 , 5a , 5a2 , 5a3

5b ©

5b4 ©

5b5 ©

5b6 ©

Specimen 5b: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Double labeled: blue - Gata6; red - Snx1 (assay MGI:7541128).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5b4 , 5b6 , 5b5 , 5b

5c ©

5d ©

Specimen 5c/d: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab4a (assay MGI:7541131).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5c , 5d

5g ©

5h ©

Specimen 5g/h: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab4a (assay MGI:7541131).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5h , 5g

5k ©

5l ©

Specimen 5k/l: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab11a (assay MGI:7541132).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5l , 5k

5o ©

5p ©

Specimen 5o/p: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab11a (assay MGI:7541132).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5o , 5p

S3a WT left ©

S3a WT right ©

Specimen S3a WT: embryonic day 3.5 (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		S3a WT right , S3a WT left

S3a -/- left ©

Specimen S3a -/-: embryonic day 3.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		S3a -/- left , S3a -/- right	Similar as in the wild-type.

S3b WT left ©

S3b WT right ©

Specimen S3b WT: embryonic day 4.5 (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	S3b WT left , S3b WT right	Expressed in cells on the surface of the inner cell mass.

S3b -/- left ©

Specimen S3b -/-: embryonic day 4.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		S3b -/- right , S3b -/- left	Similar as in the wild-type.

S3c WT left ©

S3c WT right ©

Specimen S3c WT: embryonic day 5.0 (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3c WT left , S3c WT right	visceral endoderm

S3c -/- left ©

Specimen S3c -/-: embryonic day 5.0; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3c -/- right , S3c -/- left	Positive cells did not exhibit epithelial structure but they appeared as a clump (accumulated Sox17 too), and exhibited defects in apical-basolateral polarity.

S3d WT left ©

S3d WT right ©

Specimen S3d WT: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3d WT left , S3d WT right	visceral endoderm

S3d -/- left ©

Specimen S3d -/-: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3d -/- left , S3d -/- right	Positive cells did not exhibit epithelial structure but they appeared as a clump (accumulated Sox17 too), and exhibited defects in apical-basolateral polarity.

S5a ©

S5c ©

Specimen S5a/c: embryonic day 5.5 (more

)
Note: Double labeled: blue - Gata6; green - Lama1 (assay MGI:7541136).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		S5c , S5a

S5e ©

S5g ©

Specimen S5e/g: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Double labeled: blue - Gata6; green - Lama1 (assay MGI:7541136).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		S5g , S5e

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