Gata6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:312380 Sun-Wada GH, et al., Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm. Sci Rep. 2021 Sep 29;11(1):19355
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7541116
Gene symbol:	Gata6
Gene name:	GATA binding protein 6

Antibody:	anti-GATA6
Detection system:	Secondary antibody coupled to Cy5

Results

2a ©

2b ©

Specimen 2a/b: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; red - Cdh1 (assay MGI:7541114); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2a , 2b	visceral endoderm

2e ©

2f ©

Specimen 2e/f: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; red - Cdh1 (assay MGI:7541114); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2e , 2f	Positive cells did not exhibit epithelial structure but they appeared as a clump.

2i ©

2j ©

Specimen 2i/j: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Cdh1 (assay MGI:7541117); red - Ezr (assay MGI:7541118).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2j , 2i	visceral endoderm

2m ©

2n ©

Specimen 2m/n: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Cdh1 (assay MGI:7541117); red - Ezr (assay MGI:7541118).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2m , 2n	Positive cells did not exhibit epithelial structure but they appeared as a clump.

2q ©

2r ©

Specimen 2q/r: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Atp1a1 (assay MGI:7541122); red - Ctnnb1 (assay MGI:7541119).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2r , 2q	visceral endoderm

2u ©

2v ©

Specimen 2u/v: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Atp1a1 (assay MGI:7541122); red - Ctnnb1 (assay MGI:7541119).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		2u , 2v	Positive cells did not exhibit epithelial structure but they appeared as a clump.

3a ©

3b1 ©

3b2 ©

Specimen 3a/b: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; red - Atp6v0c (assay MGI:7541123); green - Lamp2 (assay MGI:7541124).

Structure	Level	Pattern	Image	Note
TS7: parietal endoderm	Present		3b2 , 3a
TS7: embryonic visceral endoderm	Present		3b2 , 3b1 , 3a

3c ©

3d1 ©

3d2 ©

Specimen 3c/d: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; red - Atp6v0c (assay MGI:7541123); green - Lamp2 (assay MGI:7541124).

Structure	Level	Pattern	Image	Note
TS7: parietal endoderm	Present		3c , 3d2
TS7: embryonic visceral endoderm	Present		3d2 , 3c , 3d1

4a ©

4b ©

Specimen 4a/b: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab7 (assay MGI:7541126).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4b , 4a

4e ©

4f ©

Specimen 4e/f: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab7 (assay MGI:7541126).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4e , 4f	Positive cells did not exhibit epithelial structure but they appeared as a clump.

4i ©

4j ©

Specimen 4i/j: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Stx7 (assay MGI:7541127).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4j , 4i

4m ©

4n ©

Specimen 4m/n: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Stx7 (assay MGI:7541127).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4n , 4m	Positive cells did not exhibit epithelial structure but they appeared as a clump.

5a ©

5a1 ©

5a2 ©

5a3 ©

Specimen 5a: embryonic day 5.5 (more

)
Note: Double labeled: blue - Gata6; red - Snx1 (assay MGI:7541128).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5a1 , 5a , 5a2 , 5a3

5b ©

5b4 ©

5b5 ©

5b6 ©

Specimen 5b: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Double labeled: blue - Gata6; red - Snx1 (assay MGI:7541128).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5b4 , 5b6 , 5b5 , 5b

5c ©

5d ©

Specimen 5c/d: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab4a (assay MGI:7541131).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5c , 5d

5g ©

5h ©

Specimen 5g/h: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab4a (assay MGI:7541131).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5h , 5g

5k ©

5l ©

Specimen 5k/l: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab11a (assay MGI:7541132).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5l , 5k

5o ©

5p ©

Specimen 5o/p: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Lamp2 (assay MGI:7541124); red - Rab11a (assay MGI:7541132).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5o , 5p

S3a WT left ©

S3a WT right ©

Specimen S3a WT: embryonic day 3.5 (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		S3a WT right , S3a WT left

S3a -/- left ©

Specimen S3a -/-: embryonic day 3.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		S3a -/- left , S3a -/- right	Similar as in the wild-type.

S3b WT left ©

S3b WT right ©

Specimen S3b WT: embryonic day 4.5 (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	S3b WT left , S3b WT right	Expressed in cells on the surface of the inner cell mass.

S3b -/- left ©

Specimen S3b -/-: embryonic day 4.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		S3b -/- right , S3b -/- left	Similar as in the wild-type.

S3c WT left ©

S3c WT right ©

Specimen S3c WT: embryonic day 5.0 (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3c WT left , S3c WT right	visceral endoderm

S3c -/- left ©

Specimen S3c -/-: embryonic day 5.0; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3c -/- right , S3c -/- left	Positive cells did not exhibit epithelial structure but they appeared as a clump (accumulated Sox17 too), and exhibited defects in apical-basolateral polarity.

S3d WT left ©

S3d WT right ©

Specimen S3d WT: embryonic day 5.5 (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3d WT left , S3d WT right	visceral endoderm

S3d -/- left ©

Specimen S3d -/-: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Gata6; green - Prkcz (assay MGI:7541115); red - Sox17 (assay MGI:7541133).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3d -/- left , S3d -/- right	Positive cells did not exhibit epithelial structure but they appeared as a clump (accumulated Sox17 too), and exhibited defects in apical-basolateral polarity.

S5a ©

S5c ©

Specimen S5a/c: embryonic day 5.5 (more

)
Note: Double labeled: blue - Gata6; green - Lama1 (assay MGI:7541136).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		S5c , S5a

S5e ©

S5g ©

Specimen S5e/g: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Double labeled: blue - Gata6; green - Lama1 (assay MGI:7541136).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		S5g , S5e

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24