Lamp2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:312380 Sun-Wada GH, et al., Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm. Sci Rep. 2021 Sep 29;11(1):19355
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7541124
Gene symbol:	Lamp2
Gene name:	lysosomal-associated membrane protein 2

Antibody:	anti-lamp2
Detection system:	Secondary antibody coupled to fluorescein

Results

3a ©

3b1 ©

3b2 ©

Specimen 3a/b: embryonic day 5.5 (more

)
Note: Triple labeled: green - Lamp2; red - Atp6v0c (assay MGI:7541123); blue - Gata6 (assay MGI:7541116).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		3a , 3b2 , 3b1	Expressed in the apical vacuoles (rich in lysosome).

3c ©

3d1 ©

3d2 ©

Specimen 3c/d: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: green - Lamp2; red - Atp6v0c (assay MGI:7541123); blue - Gata6 (assay MGI:7541116).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		3c , 3d2 , 3d1	Reduced expression compared with the wild-type.

3e ©

Specimen 3e: embryonic day 5.5 (more

)
Note: Triple labeled: green - Lamp2; red - Atp6v0c (assay MGI:7541123); blue - Snx1 (assay MGI:7541125).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		3e	Expressed in the apical vacuoles (rich in lysosome).

3h ©

Specimen 3h: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: green - Lamp2; red - Atp6v0c (assay MGI:7541123); blue - Snx1 (assay MGI:7541125).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		3h	Reduced expression compared with the wild-type.

4a ©

4b ©

4d ©

Specimen 4a/b/d: embryonic day 5.5 (more

)
Note: Triple labeled: green - Lamp2; blue - Gata6 (assay MGI:7541116); red - Rab7 (assay MGI:7541126).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4a , 4d , 4b	Expressed in the apical vacuole membranes (rich in lysosome).

4e ©

4f ©

4h ©

Specimen 4e/f/h: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: green - Lamp2; blue - Gata6 (assay MGI:7541116); red - Rab7 (assay MGI:7541126).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4h , 4e , 4f	Decreased expression and exhibited a dispersed pattern.

4i ©

4j ©

4l ©

Specimen 4i/j/l: embryonic day 5.5 (more

)
Note: Triple labeled: green - Lamp2; blue - Gata6 (assay MGI:7541116); red - Stx7 (assay MGI:7541127).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4j , 4l , 4i	Expressed in the apical vacuole membranes (rich in lysosome).

4m ©

4n ©

4p ©

Specimen 4m/n/p: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: green - Lamp2; blue - Gata6 (assay MGI:7541116); red - Stx7 (assay MGI:7541127).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		4n , 4m , 4p	Decreased expression and exhibited a dispersed pattern.

5c ©

5d ©

5f ©

Specimen 5c/d/f: embryonic day 5.5 (more

)
Note: Triple labeled: green - Lamp2; blue - Gata6 (assay MGI:7541116); red - Rab4a (assay MGI:7541131).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5d , 5f , 5c	Expressed in the apical vacuoles.

5g ©

5h ©

5j ©

Specimen 5g/h/j: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: green - Lamp2; blue - Gata6 (assay MGI:7541116); red - Rab4a (assay MGI:7541131).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5j , 5g , 5h	Decreased expression and exhibited a dispersed pattern.

5k ©

5l ©

5n ©

Specimen 5k/l/n: embryonic day 5.5 (more

)
Note: Triple labeled: green - Lamp2; blue - Gata6 (assay MGI:7541116); red - Rab11a (assay MGI:7541132).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5l , 5k , 5n	Expressed in the apical vacuole membranes.

5o ©

5p ©

5r ©

Specimen 5o/p/r: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: green - Lamp2; blue - Gata6 (assay MGI:7541116); red - Rab11a (assay MGI:7541132).

Structure	Level	Pattern	Image	Note
TS7: embryonic visceral endoderm	Present		5r , 5p , 5o	Decreased expression and exhibited a dispersed pattern.

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